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Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells

  • Authors:
    • Lan Luan
    • Qiang Hu
    • Yan Wang
    • Lu Lu
    • Jiaojiao Ling
  • View Affiliations

    Affiliations: Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Published online on: February 18, 2021     https://doi.org/10.3892/etm.2021.9798
  • Article Number: 367
  • Copyright: © Luan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Retinoblastoma (RB) is the most common primary intraocular cancer type that occurs during retinal development in childhood. Previous studies have reported that long non‑coding RNAs (lncRNAs) are involved in the development of RB. Therefore, the aim of the present study was to investigate the effects and underlying regulatory mechanisms of nuclear paraspeckle assembly transcript 1 (NEAT1) in RB. The expression levels of NEAT1, microRNA (miR)‑24‑3p and leucine‑rich‑α‑2‑glycoprotein (LRG1) were detected using reverse transcription‑quantitative PCR (RT‑qPCR). Moreover, the protein expression levels of LRG1, matrix metalloproteinase 9, N‑cadherin and E‑cadherin were detected via western blotting. Furthermore, cell migration and invasion abilities were evaluated via Transwell assays. The targeting relationships between miR‑24‑3p and NEAT1 or LRG1 were predicted using online software and confirmed via dual‑luciferase reporter assay. In the present study, NEAT1 and LRG1 were upregulated, and miR‑24‑3p was downregulated in RB tissues and cells compared with the corresponding healthy tissues and cells. Moreover, miR‑24‑3p was identified as a target of NEAT and LRG1 was demonstrated to be a direct target gene of miR‑24‑3p. Knockdown of NEAT1 or LRG1 significantly suppressed RB cell migration and invasion ability, while the effects were reversed by an miR‑24‑3p inhibitor. In addition, the downregulation of LRG1 caused by miR‑24‑3p was restored following the overexpression of NEAT1 in RB cells. It was also demonstrated that NEAT1 knockdown inhibited the epithelial‑to‑mesenchymal transition (EMT) pathway by inhibiting the expression of LRG via targeting miR‑24‑3p. In conclusion, the present results suggest that silencing of NEAT1 suppresses cell migration, invasion and the EMT process by downregulating LRG1 expression via sponging miR‑24‑3p in RB, thus indicating that NEAT1 may be a potential candidate for RB treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Luan L, Hu Q, Wang Y, Lu L and Ling J: Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells. Exp Ther Med 21: 367, 2021.
APA
Luan, L., Hu, Q., Wang, Y., Lu, L., & Ling, J. (2021). Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells. Experimental and Therapeutic Medicine, 21, 367. https://doi.org/10.3892/etm.2021.9798
MLA
Luan, L., Hu, Q., Wang, Y., Lu, L., Ling, J."Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells". Experimental and Therapeutic Medicine 21.4 (2021): 367.
Chicago
Luan, L., Hu, Q., Wang, Y., Lu, L., Ling, J."Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells". Experimental and Therapeutic Medicine 21, no. 4 (2021): 367. https://doi.org/10.3892/etm.2021.9798
Copy and paste a formatted citation
x
Spandidos Publications style
Luan L, Hu Q, Wang Y, Lu L and Ling J: Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells. Exp Ther Med 21: 367, 2021.
APA
Luan, L., Hu, Q., Wang, Y., Lu, L., & Ling, J. (2021). Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells. Experimental and Therapeutic Medicine, 21, 367. https://doi.org/10.3892/etm.2021.9798
MLA
Luan, L., Hu, Q., Wang, Y., Lu, L., Ling, J."Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells". Experimental and Therapeutic Medicine 21.4 (2021): 367.
Chicago
Luan, L., Hu, Q., Wang, Y., Lu, L., Ling, J."Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR‑24‑3p/LRG1 axis in retinoblastoma cells". Experimental and Therapeutic Medicine 21, no. 4 (2021): 367. https://doi.org/10.3892/etm.2021.9798
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