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Anti‑inflammatory effects of Fritillaria thunbergii Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells

  • Authors:
    • Jae-Hyun Kim
    • Minsun Kim
    • Sooyeon Hong
    • Boguen Kwon
    • Min Wook Song
    • Kwangchan Song
    • Eun-Young Kim
    • Hyuk-Sang Jung
    • Youngjoo Sohn
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 429
    |
    Published online on: February 26, 2021
       https://doi.org/10.3892/etm.2021.9846
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Abstract

The aim of the present study was to demonstrate that Fritillaria thunbergii Miquel extract exerts anti‑inflammatory and antioxidant effects on lipopolysaccharide‑stimulated RAW 264.7 cells. To confirm the inhibitory effect of ethyl acetate fraction of FTM (EAFM) on inflammation, the expression of nitric oxide (NO) and inflammatory cytokines was assessed by performing ELISA. Expression of intracellular mRNA and protein was confirmed by reverse transcription PCR and western blotting. In addition, the anti‑inflammatory and anti‑oxidant mechanisms of NF‑κB, MAPK and heme oxygenase‑1 (HO‑1) were also investigated. EAFM significantly inhibited the expression of inflammatory factors including NO, IL‑6 and TNF‑α at non‑toxic concentrations. EAFM also inhibited the mRNA and protein expression of inducible nitric oxide synthase in a concentration‑dependent manner, but did not alter the expression of cyclooxygenase‑2. Pre‑treatment with EAFM inhibited the nuclear translocation of NF‑κB, and suppressed the phosphorylation of ERK and JNK. In addition, EAFM induced 2,2'‑azino‑bis(3‑ethylbenzothiazoline‑6‑sulfonic acid) radical scavenging activity and an increase in the expression of nuclear factor erythroid 2‑related factor 2 (Nrf2) and HO‑1. The results indicated that EAFM inhibited the expression of pro‑inflammatory cytokines by inhibiting ERK/JNK phosphorylation and NF‑κB translocation. EAFM also exerted antioxidant effects via Nrf2/HO‑1 stimulation. Collectively, the results of the present study indicated that EAFM may be a valuable alternative for the treatment of a variety of inflammatory diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Kim J, Kim M, Hong S, Kwon B, Song M, Song K, Kim E, Jung H and Sohn Y: Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells. Exp Ther Med 21: 429, 2021.
APA
Kim, J., Kim, M., Hong, S., Kwon, B., Song, M., Song, K. ... Sohn, Y. (2021). Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells. Experimental and Therapeutic Medicine, 21, 429. https://doi.org/10.3892/etm.2021.9846
MLA
Kim, J., Kim, M., Hong, S., Kwon, B., Song, M., Song, K., Kim, E., Jung, H., Sohn, Y."Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells". Experimental and Therapeutic Medicine 21.5 (2021): 429.
Chicago
Kim, J., Kim, M., Hong, S., Kwon, B., Song, M., Song, K., Kim, E., Jung, H., Sohn, Y."Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells". Experimental and Therapeutic Medicine 21, no. 5 (2021): 429. https://doi.org/10.3892/etm.2021.9846
Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Kim M, Hong S, Kwon B, Song M, Song K, Kim E, Jung H and Sohn Y: Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells. Exp Ther Med 21: 429, 2021.
APA
Kim, J., Kim, M., Hong, S., Kwon, B., Song, M., Song, K. ... Sohn, Y. (2021). Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells. Experimental and Therapeutic Medicine, 21, 429. https://doi.org/10.3892/etm.2021.9846
MLA
Kim, J., Kim, M., Hong, S., Kwon, B., Song, M., Song, K., Kim, E., Jung, H., Sohn, Y."Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells". Experimental and Therapeutic Medicine 21.5 (2021): 429.
Chicago
Kim, J., Kim, M., Hong, S., Kwon, B., Song, M., Song, K., Kim, E., Jung, H., Sohn, Y."Anti‑inflammatory effects of <em>Fritillaria thunbergii</em> Miquel extracts in LPS‑stimulated murine macrophage RAW 264.7 cells". Experimental and Therapeutic Medicine 21, no. 5 (2021): 429. https://doi.org/10.3892/etm.2021.9846
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