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Article

Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart

  • Authors:
    • Annika Raupach
    • Katharina Feige
    • Christian Reiter
    • Timo Brandenburger
    • Nicole Heinen
    • André Heinen
    • Markus W. Hollmann
    • Ragnar Huhn
    • Carolin Torregroza
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, University Hospital Duesseldorf, D‑40225 Duesseldorf, Germany, Institute of Cardiovascular Physiology, Heinrich‑Heine‑University Duesseldorf, D‑40225 Duesseldorf, Germany, Department of Anesthesiology, Amsterdam University Medical Center (AUMC), Location AMC, 1100 DD Amsterdam, The Netherlands
  • Article Number: 432
    |
    Published online on: February 26, 2021
       https://doi.org/10.3892/etm.2021.9849
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Abstract

Remote ischemic preconditioning (RIPC) is hypothesized to be a promising cardioprotective strategy to protect hearts against ischemia and reperfusion (I/R) injury; however, the current understanding of the underlying signal transduction pathways involved remains unclear. It has been previously demonstrated that protein kinase B/AKT, which is a crucial protein of the reperfusion injury salvage kinases pathway, and STAT5, which is a member of the survivor activating factor enhancement pathway, serve a pivotal role in cardioprotection. However, whether and at what time‑points (TPs) RIPC leads to the activation of AKT and STAT5 in a rat model of RIPC and I/R injury remains to be determined. The present study hypothesized that RIPC may induce the phosphorylation of AKT and/or STAT5 immediately following RIPC and/or at a later TP with or without subsequent I/R. In the first set of experiments (part A), male Wistar rats were randomized into 2 groups (n=6 per group): The first group underwent RIPC via a hind limb tourniquet (4x5 min I/R episodes), while the second group received the respective sham treatment. In the second set of experiments (part B), the rats were randomized into 4 groups (n=6 per group) that either underwent RIPC or sham treatment prior to 35 min of ischemia by occlusion of the left anterior descending coronary artery followed by 120 min reperfusion or a respective sham treatment. At the end of the experiments, the heart tissue was isolated in order to analyze the phosphorylation levels of AKT and STAT5. The results revealed that RIPC did not induce the immediate or late phosphorylation of AKT or STAT5. In addition, following I/R, the activation of AKT and STAT5 was not modulated by RIPC. In conclusion, the findings of the present study suggested that RIPC‑induced cardioprotection may not be mediated by the activation of AKT or STAT5 at the investigated TPs.
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1 

Global Health Estimates 2016: Deaths by Cause, Age, Sex, by Country and by Region, 2000-2016. Geneva, World Health Organization, 2018.

2 

Yellon DM and Hausenloy DJ: Myocardial reperfusion injury. N Engl J Med. 357:1121–1135. 2007.PubMed/NCBI View Article : Google Scholar

3 

Ferdinandy P, Hausenloy DJ, Heusch G, Baxter GF and Schulz R: Interaction of risk factors, comorbidities, and comedications with ischemia/reperfusion injury and cardioprotection by preconditioning, postconditioning, and remote conditioning. Pharmacol Rev. 66:1142–1174. 2014.PubMed/NCBI View Article : Google Scholar

4 

Murry CE, Jennings RB and Reimer KA: Preconditioning with ischemia: A delay of lethal cell injury in ischemic myocardium. Circulation. 74:1124–1136. 1986.PubMed/NCBI View Article : Google Scholar

5 

Heusch G: Molecular basis of cardioprotection: signal transduction in ischemic pre-, post-, and remote conditioning. Circ Res. 116:674–699. 2015.PubMed/NCBI View Article : Google Scholar

6 

Cho YJ and Kim WH: Perioperative cardioprotection by remote ischemic conditioning. Int J Mol Sci. 20(4839)2019.PubMed/NCBI View Article : Google Scholar

7 

Rossello X and Yellon DM: The RISK pathway and beyond. Basic Res Cardiol. 113(2)2017.PubMed/NCBI View Article : Google Scholar

8 

Li J, Xuan W, Yan R, Tropak MB, Jean-St-Michel E, Liang W, Gladstone R, Backx PH, Kharbanda RK and Redington AN: Remote preconditioning provides potent cardioprotection via PI3K/Akt activation and is associated with nuclear accumulation of β-catenin. Clin Sci (Lond). 120:451–462. 2011.PubMed/NCBI View Article : Google Scholar

9 

Hadebe N, Cour M and Lecour S: The SAFE pathway for cardioprotection: Is this a promising target? Basic Res Cardiol. 113(9)2018.PubMed/NCBI View Article : Google Scholar

10 

Wu Q, Wang T, Chen S, Zhou Q, Li H, Hu N, Feng Y, Dong N, Yao S and Xia Z: Cardiac protective effects of remote ischaemic preconditioning in children undergoing tetralogy of fallot repair surgery: A randomized controlled trial. Eur Heart J. 39:1028–1037. 2018.PubMed/NCBI View Article : Google Scholar

11 

Heusch G, Musiolik J, Kottenberg E, Peters J, Jakob H and Thielmann M: STAT5 activation and cardioprotection by remote ischemic preconditioning in humans: Short communication. Circ Res. 110:111–115. 2012.PubMed/NCBI View Article : Google Scholar

12 

Chen H, Jing XY, Shen YJ, Wang TL, Ou C, Lu SF, Cai Y, Li Q, Chen X, Ding YJ, et al: Stat5-dependent cardioprotection in late remote ischaemia preconditioning. Cardiovasc Res. 114:679–689. 2018.PubMed/NCBI View Article : Google Scholar

13 

Weber NC, Riedemann I, Smit KF, Zitta K, van de Vondervoort D, Zuurbier CJ, Hollmann MW, Preckel B and Albrecht M: Plasma from human volunteers subjected to remote ischemic preconditioning protects human endothelial cells from hypoxia-induced cell damage. Basic Res Cardiol. 110(17)2015.PubMed/NCBI View Article : Google Scholar

14 

Hildebrandt HA, Kreienkamp V, Gent S, Kahlert P, Heusch G and Kleinbongard P: Kinetics and signal activation properties of circulating factor(s) from healthy volunteers undergoing remote ischemic pre-conditioning. JACC Basic Transl Sci. 1:3–13. 2016.PubMed/NCBI View Article : Google Scholar

15 

Kilkenny C, Browne WJ, Cuthill IC, Emerson M and Altman DG: Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research. PLoS Biol. 8(e1000412)2010.PubMed/NCBI View Article : Google Scholar

16 

Brandenburger T, Huhn R, Galas A, Pannen BH, Keitel V, Barthel F, Bauer I and Heinen A: Remote ischemic preconditioning preserves connexin 43 phosphorylation in the rat heart in vivo. J Transl Med. 12(228)2014.PubMed/NCBI View Article : Google Scholar

17 

Chow SC, Shao J and Wang H: Sample Size Calculations in Clinical Research. 2nd edition. Chapman & Hall/CRC Biostatistics Series, pp58. 2008.

18 

Donato M, Goyeneche MA, Garces M, Marchini T, Pérez V, Del Mauro J, Höcht C, Rodriguez M, Evelson P and Gelpi RJ: Myocardial triggers involved in activation of remote ischaemic preconditioning. Exp Physiol. 101:708–716. 2016.PubMed/NCBI View Article : Google Scholar

19 

Skyschally A, Gent S, Amanakis G, Schulte C, Kleinbongard P and Heusch G: Across-species transfer of protection by remote ischemic preconditioning with species-specific myocardial signal transduction by reperfusion injury salvage kinase and survival activating factor enhancement pathways. Circ Res. 117:279–288. 2015.PubMed/NCBI View Article : Google Scholar

20 

Ma LL, Kong FJ, Guo JJ, Zhu JB, Shi HT, Li Y, Sun RH and Ge JB: Hypercholesterolemia abrogates remote ischemic preconditioning-induced cardioprotection: Role of reperfusion injury salvage kinase signals. Shock. 47:363–369. 2017.PubMed/NCBI View Article : Google Scholar

21 

Hong J, Ge HW, Liu JQ, Sun RH and Kong FJ: Pharmacological inhibition of PTEN restores remote ischemic postconditioning cardioprotection in hypercholesterolemic mice: Potential role of PTEN/AKT/GSK3β SIGNALS. Shock. 52:522–531. 2019.PubMed/NCBI View Article : Google Scholar

22 

Gedik N, Thielmann M, Kottenberg E, Peters J, Jakob H, Heusch G and Kleinbongard P: No evidence for activated autophagy in left ventricular myocardium at early reperfusion with protection by remote ischemic preconditioning in patients undergoing coronary artery bypass grafting. PLoS One. 9(e96567)2014.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Raupach A, Feige K, Reiter C, Brandenburger T, Heinen N, Heinen A, Hollmann MW, Huhn R and Torregroza C: Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart. Exp Ther Med 21: 432, 2021.
APA
Raupach, A., Feige, K., Reiter, C., Brandenburger, T., Heinen, N., Heinen, A. ... Torregroza, C. (2021). Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart. Experimental and Therapeutic Medicine, 21, 432. https://doi.org/10.3892/etm.2021.9849
MLA
Raupach, A., Feige, K., Reiter, C., Brandenburger, T., Heinen, N., Heinen, A., Hollmann, M. W., Huhn, R., Torregroza, C."Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart". Experimental and Therapeutic Medicine 21.5 (2021): 432.
Chicago
Raupach, A., Feige, K., Reiter, C., Brandenburger, T., Heinen, N., Heinen, A., Hollmann, M. W., Huhn, R., Torregroza, C."Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart". Experimental and Therapeutic Medicine 21, no. 5 (2021): 432. https://doi.org/10.3892/etm.2021.9849
Copy and paste a formatted citation
x
Spandidos Publications style
Raupach A, Feige K, Reiter C, Brandenburger T, Heinen N, Heinen A, Hollmann MW, Huhn R and Torregroza C: Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart. Exp Ther Med 21: 432, 2021.
APA
Raupach, A., Feige, K., Reiter, C., Brandenburger, T., Heinen, N., Heinen, A. ... Torregroza, C. (2021). Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart. Experimental and Therapeutic Medicine, 21, 432. https://doi.org/10.3892/etm.2021.9849
MLA
Raupach, A., Feige, K., Reiter, C., Brandenburger, T., Heinen, N., Heinen, A., Hollmann, M. W., Huhn, R., Torregroza, C."Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart". Experimental and Therapeutic Medicine 21.5 (2021): 432.
Chicago
Raupach, A., Feige, K., Reiter, C., Brandenburger, T., Heinen, N., Heinen, A., Hollmann, M. W., Huhn, R., Torregroza, C."Remote ischemic preconditioning does not induce activation of Akt and STAT5 in the rat heart". Experimental and Therapeutic Medicine 21, no. 5 (2021): 432. https://doi.org/10.3892/etm.2021.9849
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