Open Access

Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis

  • Authors:
    • Yang Wang
    • Xiaojia Song
    • Rui Lei
    • Ning Zhang
    • Liangping Zhang
    • Wei Xiao
    • Jinghong Xu
    • Jun Lin
  • View Affiliations

  • Published online on: March 1, 2021     https://doi.org/10.3892/etm.2021.9882
  • Article Number: 452
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Transplantation of cell‑based material is a promising approach for the treatment of critical bone defects. However, it is still limited by the lack of suitable scaffold material or abundant seeding cell sources. The present study aimed to establish a novel composite of an adipose‑derived stem cell (ADSC) sheet and a synthetic porous β‑tricalcium phosphate/collagen‑I fiber (β‑TCP/COL‑I) scaffold to enhance osteogenic activity. ADSCs were isolated from 3‑week‑old female Sprague Dawley rats and the ADSC sheets were prepared in an osteoinductive medium. The study groups included the ADSC sheets/scaffold, scattered ADSCs/scaffold, ADSC sheet alone and scaffold alone. Scanning electron microscopy and energy‑dispersive spectrometry were used to observe cell‑scaffold interactions and analyze the relative calcium content on the composites' surface. Alizarin red S staining was used to examine the calcium deposition. ELISA and reverse transcription‑quantitative PCR were used to detect the expression levels of alkaline phosphatase (ALP), osteocalcin (OCN) and osteopontin (OPN). The results revealed that ADSCs were able to tightly adhere to the β‑TCP/COL‑I scaffold with no cytotoxicity. The calcifying nodules reaction was positive on ADSC sheets and gradually increased after osteogenic induction. In addition, the β‑TCP/COL‑I scaffold combined with ADSC sheets was able to significantly enhance the expression levels of ALP, OCN and OPN and increase the superficial relative calcium content compared to scattered ADSCs/scaffold or the ADSC sheet alone (P<0.05). The results indicated that ADSCs possess a strong osteogenic potential, particularly in the cell‑sheet form and when compounded with the β‑TCP/COL‑I scaffold, compared to scattered ADSCs with a β‑TCP/COL‑I scaffold or an ADSC sheet alone. This novel composite may be a promising candidate for bone engineering.
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May-2021
Volume 21 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang Y, Song X, Lei R, Zhang N, Zhang L, Xiao W, Xu J and Lin J: Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis. Exp Ther Med 21: 452, 2021
APA
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W. ... Lin, J. (2021). Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis. Experimental and Therapeutic Medicine, 21, 452. https://doi.org/10.3892/etm.2021.9882
MLA
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W., Xu, J., Lin, J."Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis". Experimental and Therapeutic Medicine 21.5 (2021): 452.
Chicago
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W., Xu, J., Lin, J."Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis". Experimental and Therapeutic Medicine 21, no. 5 (2021): 452. https://doi.org/10.3892/etm.2021.9882