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Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis

Corrigendum in: /10.3892/etm.2025.12993
  • Authors:
    • Yang Wang
    • Xiaojia Song
    • Rui Lei
    • Ning Zhang
    • Liangping Zhang
    • Wei Xiao
    • Jinghong Xu
    • Jun Lin
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China, Department of Orthodontics, Hangzhou Stomatology Hospital, Hangzhou, Zhejiang 310012, P.R. China, Dental Department, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China, Department of Plastic Surgery, The First Hospital of Jiaxing, Jiaxing, Zhejiang 314000, P.R. China, Department of Stomatology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 452
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    Published online on: March 1, 2021
       https://doi.org/10.3892/etm.2021.9882
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Abstract

Transplantation of cell‑based material is a promising approach for the treatment of critical bone defects. However, it is still limited by the lack of suitable scaffold material or abundant seeding cell sources. The present study aimed to establish a novel composite of an adipose‑derived stem cell (ADSC) sheet and a synthetic porous β‑tricalcium phosphate/collagen‑I fiber (β‑TCP/COL‑I) scaffold to enhance osteogenic activity. ADSCs were isolated from 3‑week‑old female Sprague Dawley rats and the ADSC sheets were prepared in an osteoinductive medium. The study groups included the ADSC sheets/scaffold, scattered ADSCs/scaffold, ADSC sheet alone and scaffold alone. Scanning electron microscopy and energy‑dispersive spectrometry were used to observe cell‑scaffold interactions and analyze the relative calcium content on the composites' surface. Alizarin red S staining was used to examine the calcium deposition. ELISA and reverse transcription‑quantitative PCR were used to detect the expression levels of alkaline phosphatase (ALP), osteocalcin (OCN) and osteopontin (OPN). The results revealed that ADSCs were able to tightly adhere to the β‑TCP/COL‑I scaffold with no cytotoxicity. The calcifying nodules reaction was positive on ADSC sheets and gradually increased after osteogenic induction. In addition, the β‑TCP/COL‑I scaffold combined with ADSC sheets was able to significantly enhance the expression levels of ALP, OCN and OPN and increase the superficial relative calcium content compared to scattered ADSCs/scaffold or the ADSC sheet alone (P<0.05). The results indicated that ADSCs possess a strong osteogenic potential, particularly in the cell‑sheet form and when compounded with the β‑TCP/COL‑I scaffold, compared to scattered ADSCs with a β‑TCP/COL‑I scaffold or an ADSC sheet alone. This novel composite may be a promising candidate for bone engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Song X, Lei R, Zhang N, Zhang L, Xiao W, Xu J and Lin J: Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993. Exp Ther Med 21: 452, 2021.
APA
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W. ... Lin, J. (2021). Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993. Experimental and Therapeutic Medicine, 21, 452. https://doi.org/10.3892/etm.2021.9882
MLA
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W., Xu, J., Lin, J."Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993". Experimental and Therapeutic Medicine 21.5 (2021): 452.
Chicago
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W., Xu, J., Lin, J."Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993". Experimental and Therapeutic Medicine 21, no. 5 (2021): 452. https://doi.org/10.3892/etm.2021.9882
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Song X, Lei R, Zhang N, Zhang L, Xiao W, Xu J and Lin J: Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993. Exp Ther Med 21: 452, 2021.
APA
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W. ... Lin, J. (2021). Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993. Experimental and Therapeutic Medicine, 21, 452. https://doi.org/10.3892/etm.2021.9882
MLA
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W., Xu, J., Lin, J."Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993". Experimental and Therapeutic Medicine 21.5 (2021): 452.
Chicago
Wang, Y., Song, X., Lei, R., Zhang, N., Zhang, L., Xiao, W., Xu, J., Lin, J."Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis Corrigendum in /10.3892/etm.2025.12993". Experimental and Therapeutic Medicine 21, no. 5 (2021): 452. https://doi.org/10.3892/etm.2021.9882
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