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microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12

  • Authors:
    • Bo Gao
    • Guomin Sun
    • Yan Wang
    • Yaqin Geng
    • Lei Zhou
    • Xi Chen
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatology and Immunology, The Second Changzhou People's Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu 213004, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 459
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    Published online on: March 3, 2021
       https://doi.org/10.3892/etm.2021.9890
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Abstract

Rheumatoid arthritis (RA) is a common systemic, inflammatory and autoimmune disorder. MicroRNAs (miRs) are strongly associated with the initiation and progression of RA. However, the functions and mechanisms underlying miR‑23 in RA are not completely understood. Therefore, the present study aimed to investigate the molecular mechanisms underlying miR‑23 in RA. A bioinformatics tool (StarBase) and a wide range of experimental assays, including reverse transcription‑quantitative PCR, western blotting, luciferase reporter assays and ELISAs, were performed to investigate the biological role of miR‑23 in RA. The results indicated that miR‑23 was downregulated and chemokine C‑X‑C motif ligand 12 (CXCL12) was upregulated in RA samples compared with healthy samples. Furthermore, miR‑23 overexpression suppressed inflammation via reducing TNF‑α, IL‑1β and IL‑8 expression levels compared with the NC mimic group. Regarding the underlying mechanism, compared with NC mimic, miR‑23 mimic decreased CXCL12 mRNA expression by binding to its 3'‑untranslated region. Additionally, CXCL12 overexpression reversed miR‑23 mimic‑mediated effects on inflammation. NF‑κB signaling is associated with inflammation. Therefore, the present study indicated that CXCL12 promoted inflammation by activating NF‑κB signaling. In conclusion, miR‑23 inhibited inflammation to alleviate RA by regulating CXCL12 via the NF‑κB signaling pathway, which may serve as a potential target for the diagnosis and treatment of RA.
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Copy and paste a formatted citation
Spandidos Publications style
Gao B, Sun G, Wang Y, Geng Y, Zhou L and Chen X: microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12. Exp Ther Med 21: 459, 2021.
APA
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., & Chen, X. (2021). microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12. Experimental and Therapeutic Medicine, 21, 459. https://doi.org/10.3892/etm.2021.9890
MLA
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., Chen, X."microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12". Experimental and Therapeutic Medicine 21.5 (2021): 459.
Chicago
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., Chen, X."microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12". Experimental and Therapeutic Medicine 21, no. 5 (2021): 459. https://doi.org/10.3892/etm.2021.9890
Copy and paste a formatted citation
x
Spandidos Publications style
Gao B, Sun G, Wang Y, Geng Y, Zhou L and Chen X: microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12. Exp Ther Med 21: 459, 2021.
APA
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., & Chen, X. (2021). microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12. Experimental and Therapeutic Medicine, 21, 459. https://doi.org/10.3892/etm.2021.9890
MLA
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., Chen, X."microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12". Experimental and Therapeutic Medicine 21.5 (2021): 459.
Chicago
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., Chen, X."microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12". Experimental and Therapeutic Medicine 21, no. 5 (2021): 459. https://doi.org/10.3892/etm.2021.9890
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