Open Access

microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12

  • Authors:
    • Bo Gao
    • Guomin Sun
    • Yan Wang
    • Yaqin Geng
    • Lei Zhou
    • Xi Chen
  • View Affiliations

  • Published online on: March 3, 2021     https://doi.org/10.3892/etm.2021.9890
  • Article Number: 459
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rheumatoid arthritis (RA) is a common systemic, inflammatory and autoimmune disorder. MicroRNAs (miRs) are strongly associated with the initiation and progression of RA. However, the functions and mechanisms underlying miR‑23 in RA are not completely understood. Therefore, the present study aimed to investigate the molecular mechanisms underlying miR‑23 in RA. A bioinformatics tool (StarBase) and a wide range of experimental assays, including reverse transcription‑quantitative PCR, western blotting, luciferase reporter assays and ELISAs, were performed to investigate the biological role of miR‑23 in RA. The results indicated that miR‑23 was downregulated and chemokine C‑X‑C motif ligand 12 (CXCL12) was upregulated in RA samples compared with healthy samples. Furthermore, miR‑23 overexpression suppressed inflammation via reducing TNF‑α, IL‑1β and IL‑8 expression levels compared with the NC mimic group. Regarding the underlying mechanism, compared with NC mimic, miR‑23 mimic decreased CXCL12 mRNA expression by binding to its 3'‑untranslated region. Additionally, CXCL12 overexpression reversed miR‑23 mimic‑mediated effects on inflammation. NF‑κB signaling is associated with inflammation. Therefore, the present study indicated that CXCL12 promoted inflammation by activating NF‑κB signaling. In conclusion, miR‑23 inhibited inflammation to alleviate RA by regulating CXCL12 via the NF‑κB signaling pathway, which may serve as a potential target for the diagnosis and treatment of RA.
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May-2021
Volume 21 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Gao B, Sun G, Wang Y, Geng Y, Zhou L and Chen X: microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12. Exp Ther Med 21: 459, 2021
APA
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., & Chen, X. (2021). microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12. Experimental and Therapeutic Medicine, 21, 459. https://doi.org/10.3892/etm.2021.9890
MLA
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., Chen, X."microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12". Experimental and Therapeutic Medicine 21.5 (2021): 459.
Chicago
Gao, B., Sun, G., Wang, Y., Geng, Y., Zhou, L., Chen, X."microRNA‑23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12". Experimental and Therapeutic Medicine 21, no. 5 (2021): 459. https://doi.org/10.3892/etm.2021.9890