Open Access

Quercetin suppresses apoptosis of chondrocytes induced by IL‑1β via inactivation of p38 MAPK signaling pathway

  • Authors:
    • Xiang-Peng Wang
    • Wen-Peng Xie
    • Yi-Fei Bi
    • Bao-An Wang
    • Hong-Bo Song
    • Shi-Lu Wang
    • Rong-Xiu Bi
  • View Affiliations

  • Published online on: March 8, 2021     https://doi.org/10.3892/etm.2021.9899
  • Article Number: 468
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The objective of the present study was to investigate the effect of quercetin and evaluate its protective effect on articular cartilage in patients with osteoarthritis (OA), by intervening the p38 pathway. The target factors of quercetin protecting articular cartilage in patients with OA were predicted scientifically and analyzed to predict the possible pathways by using network pharmacology. A pathway predicted to be closely associated with osteoarthritis was chosen for experimental verification in in vitro cells. The optimal intervention drug concentrations were selected by the of Cell Cycle Kit‑8 assay, osteoarthritis and inflammatory factors relevant to osteoarthritis, interleukin‑1β and tumor necrosis factor‑α, were tested by of enzyme‑linked immunosorbent assay, and the expression of relevant proteins and mRNA of the p38 signaling pathway was tested by reverse transcription‑quantitative PCR and western blotting, following quercetin intervention. It was found that quercetin, at the concentration of 100 umol/l, can decrease inflammatory factors relevant to OA, inhibit the expression of p38, matrix metalloprotease 13 and ADAMTS in the pathway, and promote the expression of collagen Ⅱ. Therefore, it is postulated that quercetin can lower the expression of inflammatory factors in cartilage for the prevention and treatment of OA, and the expression level of relevant factors can be changed positively by blocking the p38 MAPK signaling pathway. Thus, quercetin can promote the repair of degenerative chondrocytes and protect articular chondrocytes.
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May-2021
Volume 21 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang X, Xie W, Bi Y, Wang B, Song H, Wang S and Bi R: Quercetin suppresses apoptosis of chondrocytes induced by IL‑1β via inactivation of p38 MAPK signaling pathway. Exp Ther Med 21: 468, 2021
APA
Wang, X., Xie, W., Bi, Y., Wang, B., Song, H., Wang, S., & Bi, R. (2021). Quercetin suppresses apoptosis of chondrocytes induced by IL‑1β via inactivation of p38 MAPK signaling pathway. Experimental and Therapeutic Medicine, 21, 468. https://doi.org/10.3892/etm.2021.9899
MLA
Wang, X., Xie, W., Bi, Y., Wang, B., Song, H., Wang, S., Bi, R."Quercetin suppresses apoptosis of chondrocytes induced by IL‑1β via inactivation of p38 MAPK signaling pathway". Experimental and Therapeutic Medicine 21.5 (2021): 468.
Chicago
Wang, X., Xie, W., Bi, Y., Wang, B., Song, H., Wang, S., Bi, R."Quercetin suppresses apoptosis of chondrocytes induced by IL‑1β via inactivation of p38 MAPK signaling pathway". Experimental and Therapeutic Medicine 21, no. 5 (2021): 468. https://doi.org/10.3892/etm.2021.9899