Influence of the microenvironment dynamics on extracellular matrix evolution under hypoxic ischemic conditions in the myocardium

Ceauşu,Zenaida; Popa,Manuela; Socea,Bogdan; Gorecki,Gabriel Petre; Costache,Mariana; Ceauşu,Mihai

doi:10.3892/etm.2022.11122

Influence of the microenvironment dynamics on extracellular matrix evolution under hypoxic ischemic conditions in the myocardium

Authors:
- Zenaida Ceauşu
- Manuela Popa
- Bogdan Socea
- Gabriel Petre Gorecki
- Mariana Costache
- Mihai Ceauşu
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Affiliations: Pathology Department, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania, Department of Surgery, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania, Department of Anesthesiology, ‘Sf. Pantelimon’ Emergency Hospital, 021659 Bucharest, Romania
Published online on: January 5, 2022 https://doi.org/10.3892/etm.2022.11122
Article Number: 199

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Abstract

The extracellular matrix (ECM) consists of ﬁbrillary and non‑ﬁbrillary components in the extracellular zone, and fulfills structural and signaling roles. Cardiac insult can lead to cardiomyocyte death, which subsequently determines dynamic changes of ECM composition and regulates cellular responses, ultimately contributing to cardiac repair. The present retrospective study on a small batch selected from the database of the Pathology Department of ‘Sf. Pantelimon’ Hospital aimed to determine which molecules may have a role in the dynamics of ECM using histopathology and immunohistochemistry methods. The study batch was composed of cases with cardiac ischemic conditions who died at various ages of myocardial infarcts. Tissue samples were taken from the myocardium of the left ventricle (anterior and lateral walls), and multiple series of histological sections were produced and analyzed using immunohistochemistry for collagen type I (Col‑1), tenascin C (Tn‑C), matrix metalloproteinase 9, CD34, and CD68. Col‑1 and Tn‑C showed variable patterns of fibrillar plexiform network, associated with a high micro-vascular density of newly formed capillaries revealed by CD34, and an interstitial infiltrate with histiocytes demonstrated by CD68 presence. The ECM represents therefore a polymorphic microenvironment with its own dynamics that is in continuous change, involving a large spectrum of heterogenous molecules, which play different roles in myocardium remodeling under hypoxic ischemic conditions.

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