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Article Open Access

MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells

  • Authors:
    • Manyu Liu
    • Jintao Li
    • Yangjunqi Wang
    • Maliha Ghaffar
    • Yishu Yang
    • Minglian Wang
    • Changshuo Li
  • View Affiliations / Copyright

    Affiliations: Beijing Key Laboratory of Environmental and Viral Oncology, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 204
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    Published online on: January 7, 2022
       https://doi.org/10.3892/etm.2022.11127
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Abstract

The melanoma antigen gene family A (MAGEA) family of proteins comprises of cancer‑testis antigens that are highly expressed in a number of tumours but are minimally expressed in normal cells. Due to its expression characteristics, this protein family has become a popular target for anti‑cancer drugs and immunotherapy research over recent years. Although, elevated expression levels of MAGEA6 has been found in different types of tumours, there remains to be insufficient information on the function of MAGEA6 and its associated gene regulation pathways. The present study used Transwell, Cell Counting Kit‑8 and wound healing assays to analyse the effects of MAGEA6 on Eca109 cell invasion, migration and proliferation. The main functions and pathways involved in MAGEA6 were predicted by Illumina Hiseq screening for mutually regulated genes and core genes. Eca109 cell line with a high expression of MAGEA6 was a stable cell line obtained by transfection in the early stage, and this cell line was used in subsequent experiments. Transcriptome sequencing was performed on this cell line and the Eca109 cell line that normally expressed MAGEA6. It was revealed that a high expression of MAGEA6 conferred a significant stimulating effect on cell proliferation whilst also significantly increasing cell invasion and migration. Transcriptomic analysis identified 14 differentially expressed genes and 13 core regulatory genes closely associated with MAGEA6 expression regulation, such as methylsterol monooxygenase 1 (MSMO1). The present study suggest that MAGEA6 positively regulated MSMO1 expression, which may serve an oncogenic role in cells through this regulatory effect. Overall, this provided a novel route of investigation for an in‑depth study of the regulatory function of MAGEA6.
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Copy and paste a formatted citation
Spandidos Publications style
Liu M, Li J, Wang Y, Ghaffar M, Yang Y, Wang M and Li C: MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells. Exp Ther Med 23: 204, 2022.
APA
Liu, M., Li, J., Wang, Y., Ghaffar, M., Yang, Y., Wang, M., & Li, C. (2022). MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells. Experimental and Therapeutic Medicine, 23, 204. https://doi.org/10.3892/etm.2022.11127
MLA
Liu, M., Li, J., Wang, Y., Ghaffar, M., Yang, Y., Wang, M., Li, C."MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells". Experimental and Therapeutic Medicine 23.3 (2022): 204.
Chicago
Liu, M., Li, J., Wang, Y., Ghaffar, M., Yang, Y., Wang, M., Li, C."MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells". Experimental and Therapeutic Medicine 23, no. 3 (2022): 204. https://doi.org/10.3892/etm.2022.11127
Copy and paste a formatted citation
x
Spandidos Publications style
Liu M, Li J, Wang Y, Ghaffar M, Yang Y, Wang M and Li C: MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells. Exp Ther Med 23: 204, 2022.
APA
Liu, M., Li, J., Wang, Y., Ghaffar, M., Yang, Y., Wang, M., & Li, C. (2022). MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells. Experimental and Therapeutic Medicine, 23, 204. https://doi.org/10.3892/etm.2022.11127
MLA
Liu, M., Li, J., Wang, Y., Ghaffar, M., Yang, Y., Wang, M., Li, C."MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells". Experimental and Therapeutic Medicine 23.3 (2022): 204.
Chicago
Liu, M., Li, J., Wang, Y., Ghaffar, M., Yang, Y., Wang, M., Li, C."MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells". Experimental and Therapeutic Medicine 23, no. 3 (2022): 204. https://doi.org/10.3892/etm.2022.11127
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