The anti‑inflammation effect of strontium ranelate on rat chondrocytes with or without IL‑1β <em>in vitro</em>

Yu,Hao; Liu,Yan; Yang,Xiangwen; He,Jiajing; Zhong,Qun; Guo,Xiaojing

doi:10.3892/etm.2022.11131

The anti‑inflammation effect of strontium ranelate on rat chondrocytes with or without IL‑1β in vitro

Authors:
- Hao Yu
- Yan Liu
- Xiangwen Yang
- Jiajing He
- Qun Zhong
- Xiaojing Guo
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Affiliations: Department of Prosthodontics, Shanghai Stomatological Hospital, Huangpu, Shanghai 200001, P.R. China
Published online on: January 7, 2022 https://doi.org/10.3892/etm.2022.11131
Article Number: 208
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Temporomandibular joint osteoarthritis (TMJ‑OA) is a common disease with a high level of inflammation in the joint micro‑environment and cartilage degradation. Anti‑inflammation and cartilage regeneration are the key therapies for TMJ‑OA, but currently, there are no novel medicines or treatments that can control its pathogenic progression. Strontium ranelate (SrR) is an anti‑osteoporosis drug and is now considered a promising anti‑OA drug, but the anti‑inflammatory effect of SrR remains to be elucidated. In the present study, the anti‑inflammatory effect of SrR in a normal or high IL‑1β environment was observed. Cell viability under the treatment of SrR was tested using Cell Counting Kit‑8. Toluidine blue staining, immunofluorescence staining, hydroxyproline assay, PCR assay and western blotting were used to detect the expression of collagen (Col)II, proteoglycans (PG) and aggrecan as a reflection of extracellular matrix synthesis and MMP‑9,13 hydroxyproline was used as an inflammation indicator. IL‑1β of 10 ng/ml was added to the culture medium as inflammation environment and the tests of those biomarkers were done again. Then, the changes in β‑catenin were also studied by immunofluorescence staining, PCR assay and western blotting to explore the possible involvement of the Wnt/β‑catenin pathway. The results showed a significant inhibition of MMP‑9, MMP‑13, β‑catenin and promotion of Col‑II, PG and aggrecan in normal chondrocytes. The presence of IL‑1β markedly upregulated the expression of MMP‑9, MMP‑13 and β‑catenin while suppressing Col‑II and PG and SrR partially reversed this trend. In conclusion, SrR decreased MMPs but promoted Col‑II, aggrecan and PG synthesis in rat chondrocytes with or without the presence of IL‑1β and SrR attenuated the IL‑1β‑induced increase in β‑catenin, thus reducing the inflammatory reaction.

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