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HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway

  • Authors:
    • Minyuan Nie
    • Heng Li
    • Puhe Liu
    • Ping Dang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, PLA Strategic Support Force Characteristic Medical Center, Beijing 100101, P.R. China, Department of Paediatric Dentistry, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210008, P.R. China, Department of Stomatology, The First Dental Hospital, Wuhai, Inner Mongolia Autonomous Region 016000, P.R. China, Department of Stomatology, Amcare Women's and Children's Hospital, Beijing 100016, P.R. China
    Copyright: © Nie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 224
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    Published online on: January 17, 2022
       https://doi.org/10.3892/etm.2022.11148
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Abstract

Homeobox containing 1 (HMBOX1) is a member of the homeobox transcription factor family that has been reported to serve an important role in numerous biological processes. The present study aimed to determine the role of HMBOX1 in the pathogenesis of periodontitis. Human periodontal ligament stem cells (hPDLSCs) were treated with liposaccharide (LPS) and transfected with a HMBOX1 overexpression (Ov‑HMBOX1) plasmid or small interfering (si)‑C‑X‑C motif chemokine ligand 10 (CXCL10) plasmids. The effects of Ov‑HMBOX1 on cell proliferation, inflammation and apoptosis were subsequently investigated using Cell Counting Kit‑8, ELISA for analysis of IL‑6, TNF‑α and IL‑1β levels, TUNEL and western blotting assays for analysis of Bcl‑2, Bax, cleaved caspase‑3 and caspase‑3 levels, respectively. Furthermore, the potential effects of HMBOX1 on the mRNA and protein levels of CXCL10 and the NF‑κB signaling pathway were investigated by using reverse transcription‑quantitative PCR and western blotting. Finally, the physiological processes of lipopolysaccharide (LPS)‑induced hPDLSCs overexpressing HMBOX1 were assessed following treatment with phorbol 12‑myristate 13‑acetate (PMA), a NF‑κB agonist. The results revealed that Ov‑HMBOX1 transfection promoted proliferation whilst alleviating inflammation and apoptosis in LPS‑induced hPDLSCs. Ov‑HMBOX1 reduced the expression of CXCL10 by suppressing the NF‑κB signaling pathway. PMA treatment inhibited the proliferation of LPS‑induced hPDLSCs transfected with Ov‑HMBOX1, which was reversed by transfection with si‑CXCL10. In conclusion, results of the present study provided evidence that HMBOX1 can attenuate LPS‑induced hPDLSC injury by downregulating CXCL10 expression via the NF‑κB signaling pathway, which may provide a novel insight into the development of potentially novel treatment strategies for periodontitis.
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Copy and paste a formatted citation
Spandidos Publications style
Nie M, Li H, Liu P and Dang P: HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway. Exp Ther Med 23: 224, 2022.
APA
Nie, M., Li, H., Liu, P., & Dang, P. (2022). HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 23, 224. https://doi.org/10.3892/etm.2022.11148
MLA
Nie, M., Li, H., Liu, P., Dang, P."HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23.3 (2022): 224.
Chicago
Nie, M., Li, H., Liu, P., Dang, P."HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23, no. 3 (2022): 224. https://doi.org/10.3892/etm.2022.11148
Copy and paste a formatted citation
x
Spandidos Publications style
Nie M, Li H, Liu P and Dang P: HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway. Exp Ther Med 23: 224, 2022.
APA
Nie, M., Li, H., Liu, P., & Dang, P. (2022). HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 23, 224. https://doi.org/10.3892/etm.2022.11148
MLA
Nie, M., Li, H., Liu, P., Dang, P."HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23.3 (2022): 224.
Chicago
Nie, M., Li, H., Liu, P., Dang, P."HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23, no. 3 (2022): 224. https://doi.org/10.3892/etm.2022.11148
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