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Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study

  • Authors:
    • Weiqi Wu
    • Hao Luo
    • Dan Wu
    • Menglin Shi
    • Jinhai Yu
    • Hongfei Liao
  • View Affiliations / Copyright

    Affiliations: Department of Ocular Oncology and Ocular Trauma, Affiliated Eye Hospital of Nanchang University, Jiangxi Research Institute of Ophthalmology and Visual Sciences, Key Laboratory of Ophthalmology of Jiangxi Province, Nanchang, Jiangxi 330006, P.R. China, Department of Internal Medical, Nanchang HongDu Hospital of Traditional Chinese Medicine, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 227
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    Published online on: January 18, 2022
       https://doi.org/10.3892/etm.2022.11152
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Abstract

The reduction in postoperative complications is a considerable concern after orbital reparation and reconstruction. Selecting the appropriate scaffold materials to improve the survival rates of the seeded cells is a challenge in tissue engineering. The aim of the present study was to evaluate the biological activity of a vascular endothelial cell‑hydroxyapatite orbital complex, which was constructed with tissue engineering and used as an implant after enucleation of the eyeNew Zealand white rabbits were randomly divided into two groups that underwent hydroxyapatite orbital implant surgery in the right eye. The primary orbital microvascular endothelial cells were collected from the microvascular tissue and subsequently cultured. Then, hydroxyapatite ocular implants were cultured with vascular endothelial cells in the endothelial cell (EC) group, and implants were cultured without vascular endothelial cells in the blank group. Characterization of the cells was performed with immunofluorescence staining and a Transwell migration and cell tube formation assay. The levels of interleukin‑8 (IL‑8) and vascular endothelial growth factor (VEGF) in the rabbit conjunctiva were measured with an ELISA. The results showed that the levels of IL‑8 were decreased in the EC group and increased in the blank group. The levels of VEGF were increased in the EC group when compared to the blank group with statistical significance. The average depth of the fibrovascular tissue was obviously thicker in the EC group compared with that found in the blank group. These findings suggest that the vascular endothelial cell‑hydroxyapatite orbital implant complex may be an effective strategy with which to accelerate vascularization and reduce complications of infection with satisfactory biological activity.
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Copy and paste a formatted citation
Spandidos Publications style
Wu W, Luo H, Wu D, Shi M, Yu J and Liao H: Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study. Exp Ther Med 23: 227, 2022.
APA
Wu, W., Luo, H., Wu, D., Shi, M., Yu, J., & Liao, H. (2022). Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study. Experimental and Therapeutic Medicine, 23, 227. https://doi.org/10.3892/etm.2022.11152
MLA
Wu, W., Luo, H., Wu, D., Shi, M., Yu, J., Liao, H."Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study". Experimental and Therapeutic Medicine 23.3 (2022): 227.
Chicago
Wu, W., Luo, H., Wu, D., Shi, M., Yu, J., Liao, H."Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study". Experimental and Therapeutic Medicine 23, no. 3 (2022): 227. https://doi.org/10.3892/etm.2022.11152
Copy and paste a formatted citation
x
Spandidos Publications style
Wu W, Luo H, Wu D, Shi M, Yu J and Liao H: Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study. Exp Ther Med 23: 227, 2022.
APA
Wu, W., Luo, H., Wu, D., Shi, M., Yu, J., & Liao, H. (2022). Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study. Experimental and Therapeutic Medicine, 23, 227. https://doi.org/10.3892/etm.2022.11152
MLA
Wu, W., Luo, H., Wu, D., Shi, M., Yu, J., Liao, H."Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study". Experimental and Therapeutic Medicine 23.3 (2022): 227.
Chicago
Wu, W., Luo, H., Wu, D., Shi, M., Yu, J., Liao, H."Biological activity of a vascular endothelial cell‑hydroxyapatite orbital implant complex: An experimental study". Experimental and Therapeutic Medicine 23, no. 3 (2022): 227. https://doi.org/10.3892/etm.2022.11152
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