Open Access

Study on the value of antibiotic‑resistant gene detection in Helicobacter pylori in China

  • Authors:
    • Jinfeng Dai
    • Jing Zhao
    • Liqi Mao
    • Yue Hu
    • Bin Lv
  • View Affiliations

  • Published online on: January 18, 2022     https://doi.org/10.3892/etm.2022.11153
  • Article Number: 228
  • Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to explore the value of detecting antibiotic‑resistant genes in Helicobacter pylori (H. pylori) and the association between genotype and antibiotic resistance. Two gastric mucosa samples from each H. pylori‑positive patient were collected. Each patient's H. pylori sample was cultured in vitro, and the agar plate dilution method was conducted. In addition, all patient samples were analyzed for the detection of antibiotic resistance‑related mutant genes and VacA gene genotypes. The association between VacA genotypes and antibiotic resistance was also determined and the value of mutant gene detection in predicting H. pylori resistance to antibiotics was evaluated. In total, 133 H. pylori‑positive patients were enrolled. A total of 22 strains of H. pylori failed to grow in in vitro culture and 25 strains were negative in a H. pylori gene test. Among 108 strains detected by PCR, a total of 39 VacA s1m1 strains, 69 VacA s1m2 strains and no VacA s2 strain were identified. There was no significant association between VacA genotypes and antibiotic resistance. The mutation rates of G616A in the rdxA gene, T87A, G91A, A91G and G91T in the gyrA gene and A2143G and A2142G in the 23S rRNA gene were 32.1, 32.3, 22.6, 12.9, 6.5, 81.8 and 0.0%, respectively. Among these mutant sites, the mutation coincidence rates were as follows, according to the agar plate dilution method: rdxA G616A (81.8%), gyrA G91T (66.7%), gyrA G91A (54.5%), 23 S rRNA A2143G (49.1%), gyrA T87A (45.5%), gyrA A91G (33.3%), penicillin‑binding protein 1 (PBP1) C556G (0.0%), PBP1 A562T (0.0%), PBP1 A562G (0.0%) and 16 S rRNA 926‑927 (AT‑GT) (0.0%). VacA m subtypes were not associated with H. pylori antibiotic resistance. In conclusion, the present findings suggested that the detection of related mutant genes had a clinical application value in predicting the antibiotic resistance of H. pylori, particularly resistance to clarithromycin and levofloxacin.
View Figures
View References

Related Articles

Journal Cover

March-2022
Volume 23 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Dai J, Zhao J, Mao L, Hu Y and Lv B: Study on the value of antibiotic‑resistant gene detection in <em>Helicobacter pylori</em> in China. Exp Ther Med 23: 228, 2022
APA
Dai, J., Zhao, J., Mao, L., Hu, Y., & Lv, B. (2022). Study on the value of antibiotic‑resistant gene detection in <em>Helicobacter pylori</em> in China. Experimental and Therapeutic Medicine, 23, 228. https://doi.org/10.3892/etm.2022.11153
MLA
Dai, J., Zhao, J., Mao, L., Hu, Y., Lv, B."Study on the value of antibiotic‑resistant gene detection in <em>Helicobacter pylori</em> in China". Experimental and Therapeutic Medicine 23.3 (2022): 228.
Chicago
Dai, J., Zhao, J., Mao, L., Hu, Y., Lv, B."Study on the value of antibiotic‑resistant gene detection in <em>Helicobacter pylori</em> in China". Experimental and Therapeutic Medicine 23, no. 3 (2022): 228. https://doi.org/10.3892/etm.2022.11153