Open Access

miR‑150‑5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR

  • Authors:
    • Yuan Li
    • Wufang Qi
    • Yuquan Shi
  • View Affiliations

  • Published online on: February 15, 2022     https://doi.org/10.3892/etm.2022.11213
  • Article Number: 283
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dysregulated microRNAs (miRNAs or miRs) serve potential roles in inflammatory systemic disease, including ankylosing spondylitis (AS). The aim of the present study was to investigate the potential function of miR‑150‑5p in osteogenic differentiation of AS fibroblasts and its underlying mechanism. The expression of miR‑150‑5p and vitamin D receptor (VDR) in AS joint capsules and fibroblasts was detected by reverse transcription‑quantitative (RT‑q)PCR and western blotting. Following overexpression of miR‑150‑5p, the alteration in osteogenic gene expression was detected by RT‑qPCR, western blotting and alkaline phosphatase activity assay, as well as alizarin red staining. The association between miR‑150‑5p and VDR was confirmed by luciferase assay and rescue experiments were performed. Patients with AS exhibited decreased expression of miR‑150‑5p in joint capsules. Treatment with bone morphogenic protein 2 (BMP‑2) and transforming growth factor β1 (TGF‑β1) led to downregulation of miR‑150‑5p in AS fibroblasts. Enforced expression of miR‑150‑5p attenuated osteogenic differentiation of AS fibroblasts. These results demonstrated that miR‑150‑5p inhibited osteogenic differentiation of AS fibroblasts by targeting VDR. miR‑150‑5p overexpression decreased osteogenic transformation of fibroblasts by decreasing VDR expression in AS.
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April-2022
Volume 23 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li Y, Qi W and Shi Y: miR‑150‑5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR. Exp Ther Med 23: 283, 2022.
APA
Li, Y., Qi, W., & Shi, Y. (2022). miR‑150‑5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR. Experimental and Therapeutic Medicine, 23, 283. https://doi.org/10.3892/etm.2022.11213
MLA
Li, Y., Qi, W., Shi, Y."miR‑150‑5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR". Experimental and Therapeutic Medicine 23.4 (2022): 283.
Chicago
Li, Y., Qi, W., Shi, Y."miR‑150‑5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR". Experimental and Therapeutic Medicine 23, no. 4 (2022): 283. https://doi.org/10.3892/etm.2022.11213