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Article

Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway

  • Authors:
    • Xiaoliang Yan
    • Lin Wu
    • Min Gao
    • Pengjie Yang
    • Jinjing Yang
    • Yongzhi Deng
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Cardiology, The Affiliated Wenling Hospital of Wenzhou Medical University, Wenling, Zhejiang 317500, P.R. China, Department of Cardiovascular Surgery, The Affiliated Cardiovascular Hospital of Shanxi Medical University and Shanxi Cardiovascular Hospital (Institute), Taiyuan, Shanxi 030024, P.R. China, Department of Cardiology and Central Laboratory, Shanxi Cardiovascular Hospital, Taiyuan, Shanxi 030024, P.R. China
  • Article Number: 292
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    Published online on: February 17, 2022
       https://doi.org/10.3892/etm.2022.11222
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Abstract

The recently identified adipocytokine omentin was previously found to be expressed mainly in human omental and visceral adipose tissues. As such, reduced plasma concentrations of omentin were revealed to be associated with increased risks of cardiovascular diseases. Omentin has also been previously demonstrated to exert anti‑inflammatory effects. By contrast, resistin is a protein that has been associated with obesity and type‑2 diabetes mellitus, and the serum concentration of resistin is increased significantly in these populations. Resistin is involved in mediating inflammation development, where they can promote cardiac hypertrophy in humans through toll‑like receptor 4 (TLR4)‑related signaling. In the present study, the potential effects of omentin on resistin‑induced hypertrophy in H9c2 cardiomyoblasts were investigated. In the absence/presence of omentin, H9c2 cardiomyoblasts were treated with resistin. Omentin was found to significantly inhibit resistin‑induced increases in the surface area of H9c2 cardiomyoblasts as determined by immunofluorescence. In addition, omentin significantly inhibited resistin‑induced increases in the mRNA expression of atrial natriuretic factor, brain natriuretic peptide, β‑myosin heavy chain (which is a characteristic feature of cardiac hypertrophy) and TLR4, which was determined using reverse-transcription‑quantitative PCR. According to western blotting results, omentin significantly inhibited resistin‑induced ERK phosphorylation, which is an important mediator of cardiomyoblast hypertrophy. Furthermore, omentin significantly inhibited resistin‑induced protein expression of TLR4, myeloid differentiation factor 88 (MyD88) and NF‑κB phosphorylation, both of which are important members of inflammatory signaling. To conclude, data from the present study suggest that omentin can inhibit resistin‑induced H9c2 cardiomyoblast hypertrophy through inhibition of the TLR4/MyD88/NF‑κB signaling pathway. Therefore, omentin serve as an attractive therapeutic target against resistin‑induced cardiac hypertrophy.
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Copy and paste a formatted citation
Spandidos Publications style
Yan X, Wu L, Gao M, Yang P, Yang J and Deng Y: Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway. Exp Ther Med 23: 292, 2022.
APA
Yan, X., Wu, L., Gao, M., Yang, P., Yang, J., & Deng, Y. (2022). Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 23, 292. https://doi.org/10.3892/etm.2022.11222
MLA
Yan, X., Wu, L., Gao, M., Yang, P., Yang, J., Deng, Y."Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23.4 (2022): 292.
Chicago
Yan, X., Wu, L., Gao, M., Yang, P., Yang, J., Deng, Y."Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23, no. 4 (2022): 292. https://doi.org/10.3892/etm.2022.11222
Copy and paste a formatted citation
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Spandidos Publications style
Yan X, Wu L, Gao M, Yang P, Yang J and Deng Y: Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway. Exp Ther Med 23: 292, 2022.
APA
Yan, X., Wu, L., Gao, M., Yang, P., Yang, J., & Deng, Y. (2022). Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 23, 292. https://doi.org/10.3892/etm.2022.11222
MLA
Yan, X., Wu, L., Gao, M., Yang, P., Yang, J., Deng, Y."Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23.4 (2022): 292.
Chicago
Yan, X., Wu, L., Gao, M., Yang, P., Yang, J., Deng, Y."Omentin inhibits the resistin‑induced hypertrophy of H9c2 cardiomyoblasts by inhibiting the TLR4/MyD88/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 23, no. 4 (2022): 292. https://doi.org/10.3892/etm.2022.11222
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