miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway

Liang,Daning; Song,Guodong; Zhang,Zhenning

doi:10.3892/etm.2022.11238

miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway

Authors:
- Daning Liang
- Guodong Song
- Zhenning Zhang
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Affiliations: Department of Medical Beauty, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong 518107, P.R. China, Department of Craniomaxillofacial Surgery, Plastic Surgery Hospital of Chinese Academy of Medical Sciences, Beijing 100144, P.R. China, Department of Medical Beauty, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong 518107, P.R. China
Published online on: February 24, 2022 https://doi.org/10.3892/etm.2022.11238
Article Number: 309

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Abstract

The current study aimed to investigate the potential function and mechanism of microRNA (miR)‑216a‑3p in the osteogenic differentiation of human adipose‑derived stem cells (hADSCs). Dynamic expression changes of miR‑216a‑3p in the osteogenic differentiation of hADSCs were examined by reverse transcription‑quantitative PCR (RT‑qPCR). Regulatory effects of miR‑216a‑3p on the relative levels of osteogenesis‑associated genes were also detected by RT‑qPCR and western blotting. The relationship between miR‑216a‑3p and Wnt3a was verified through a dual‑luciferase reporter assay. Furthermore, the influence of miR‑216a‑3p on the Wnt/β‑catenin signaling pathway during the osteogenic differentiation of hADSCs was investigated by western blotting. The results revealed that during the osteogenic differentiation process of hADSCs, miR‑216a‑3p was downregulated and Wnt3a was upregulated. It was further verified that Wnt3a was the target of miR‑216a‑3p. Through inactivation of the Wnt/β‑catenin signaling pathway, miR‑216a‑3p was able to mediate osteogenic differentiation of hADSCs. In conclusion, by targeting Wnt3a, miR‑216a‑3p mediated the osteogenic differentiation of hADSCs, which negatively regulated the Wnt/β‑catenin signaling pathway.

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