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Article

miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway

  • Authors:
    • Daning Liang
    • Guodong Song
    • Zhenning Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Medical Beauty, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong 518107, P.R. China, Department of Craniomaxillofacial Surgery, Plastic Surgery Hospital of Chinese Academy of Medical Sciences, Beijing 100144, P.R. China, Department of Medical Beauty, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong 518107, P.R. China
  • Article Number: 309
    |
    Published online on: February 24, 2022
       https://doi.org/10.3892/etm.2022.11238
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Abstract

The current study aimed to investigate the potential function and mechanism of microRNA (miR)‑216a‑3p in the osteogenic differentiation of human adipose‑derived stem cells (hADSCs). Dynamic expression changes of miR‑216a‑3p in the osteogenic differentiation of hADSCs were examined by reverse transcription‑quantitative PCR (RT‑qPCR). Regulatory effects of miR‑216a‑3p on the relative levels of osteogenesis‑associated genes were also detected by RT‑qPCR and western blotting. The relationship between miR‑216a‑3p and Wnt3a was verified through a dual‑luciferase reporter assay. Furthermore, the influence of miR‑216a‑3p on the Wnt/β‑catenin signaling pathway during the osteogenic differentiation of hADSCs was investigated by western blotting. The results revealed that during the osteogenic differentiation process of hADSCs, miR‑216a‑3p was downregulated and Wnt3a was upregulated. It was further verified that Wnt3a was the target of miR‑216a‑3p. Through inactivation of the Wnt/β‑catenin signaling pathway, miR‑216a‑3p was able to mediate osteogenic differentiation of hADSCs. In conclusion, by targeting Wnt3a, miR‑216a‑3p mediated the osteogenic differentiation of hADSCs, which negatively regulated the Wnt/β‑catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liang D, Song G and Zhang Z: miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway. Exp Ther Med 23: 309, 2022.
APA
Liang, D., Song, G., & Zhang, Z. (2022). miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 23, 309. https://doi.org/10.3892/etm.2022.11238
MLA
Liang, D., Song, G., Zhang, Z."miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 23.4 (2022): 309.
Chicago
Liang, D., Song, G., Zhang, Z."miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 23, no. 4 (2022): 309. https://doi.org/10.3892/etm.2022.11238
Copy and paste a formatted citation
x
Spandidos Publications style
Liang D, Song G and Zhang Z: miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway. Exp Ther Med 23: 309, 2022.
APA
Liang, D., Song, G., & Zhang, Z. (2022). miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 23, 309. https://doi.org/10.3892/etm.2022.11238
MLA
Liang, D., Song, G., Zhang, Z."miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 23.4 (2022): 309.
Chicago
Liang, D., Song, G., Zhang, Z."miR‑216a‑3p inhibits osteogenic differentiation of human adipose‑derived stem cells via Wnt3a in the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 23, no. 4 (2022): 309. https://doi.org/10.3892/etm.2022.11238
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