Open Access

Prohibitin inhibits high glucose‑induced apoptosis via maintaining mitochondrial function in human retinal capillary endothelial cells

  • Authors:
    • Li Zhang
    • Ying He
  • View Affiliations

  • Published online on: May 5, 2022     https://doi.org/10.3892/etm.2022.11354
  • Article Number: 427
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mitochondrial dysfunction and excessive apoptosis of vascular endothelial cells play a critical role in the development of diabetic retinopathy (DR). Prohibitin (PHB), a significant regulator, maintains mitochondrial function and protects vascular endothelial cells against apoptosis. However, the mechanism underlying the protective effect of PHB on DR remains unclear. Since mitochondria are key regulators of vascular homeostasis, the present study aimed to investigate the molecular mechanism of PHB on maintaining mitochondrial function in human retinal capillary endothelial cells (HRCECs). To evaluate the role of PHB in cell apoptosis, HRCECs, transfected with or without PHB overexpression plasmid or small interfering RNA clones targeting PHB, were cultured in the presence of 5.5 mmol/l normal glucose (NG) or 30 mmol/l high glucose (HG). Subsequently, the apoptosis rate of HRCECs was determined using flow cytometry. The results showed that PHB was upregulated in HRCECs, while PHB knockdown promoted the generation of reactive oxygen species from mitochondria via inhibition of the activation of complex I. Additionally, the apoptosis rate of HRCECs in the HG group was notably enhanced compared with that in the NG group. Interestingly, PHB overexpression attenuated the increase in HG‑mediated HRCEC apoptosis. Furthermore, treatment with HG upregulated expression of cleaved caspase‑3 and cleaved poly(ADP‑ribose) polymerase in vitro. The present study indicated that PHB could be a key modulator of mitochondrial homeostasis and could protect HRCECs against HG‑induced apoptosis. Overall, the aforementioned findings provided experimental evidence supporting the potential protective effects of PHB on DR.

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June-2022
Volume 23 Issue 6

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Spandidos Publications style
Zhang L and Zhang L: Prohibitin inhibits high glucose‑induced apoptosis via maintaining mitochondrial function in human retinal capillary endothelial cells. Exp Ther Med 23: 427, 2022
APA
Zhang, L., & Zhang, L. (2022). Prohibitin inhibits high glucose‑induced apoptosis via maintaining mitochondrial function in human retinal capillary endothelial cells. Experimental and Therapeutic Medicine, 23, 427. https://doi.org/10.3892/etm.2022.11354
MLA
Zhang, L., He, Y."Prohibitin inhibits high glucose‑induced apoptosis via maintaining mitochondrial function in human retinal capillary endothelial cells". Experimental and Therapeutic Medicine 23.6 (2022): 427.
Chicago
Zhang, L., He, Y."Prohibitin inhibits high glucose‑induced apoptosis via maintaining mitochondrial function in human retinal capillary endothelial cells". Experimental and Therapeutic Medicine 23, no. 6 (2022): 427. https://doi.org/10.3892/etm.2022.11354