Systemic injury caused by taurocholate‑induced severe acute pancreatitis in rats

Hong,Xin-Xin; Wang,Hong-Yan; Yang,Jiong-Ming; Lin,Bao-Fu; Min,Qin-Qin; Liang,Yi-Zhong; Huang,Pei-Di; Zhong,Zi-You; Guo,Shao-Ju; Huang,Bin; Xu,Yi-Fei

doi:10.3892/etm.2022.11395

Systemic injury caused by taurocholate‑induced severe acute pancreatitis in rats

Authors:
- Xin-Xin Hong
- Hong-Yan Wang
- Jiong-Ming Yang
- Bao-Fu Lin
- Qin-Qin Min
- Yi-Zhong Liang
- Pei-Di Huang
- Zi-You Zhong
- Shao-Ju Guo
- Bin Huang
- Yi-Fei Xu
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Affiliations: Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518033, P.R. China, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China
Published online on: May 26, 2022 https://doi.org/10.3892/etm.2022.11395
Article Number: 468
Copyright: © Hong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Systemic injury plays a central role in severe acute pancreatitis (SAP). Retrograde biliopancreatic duct infusion of sodium taurocholate (NaT) is commonly used to establish SAP animal models. To better characterize the systemic injury in this model, SAP was induced in Sprague‑Dawley rats by NaT administration (3.5 or 5%), followed by sacrifice at 3, 6, 9, 12, 24, 48 and 72 h. Normal saline was used as a control in Sham‑operated rats. The mortality rate, ascites volume, and serum and ascitic fluid amylase and lipase activities were assessed. Multiple organ dysfunction, including dysfunction of the pancreas, lung, ileum, liver, and kidney, was investigated using hematoxylin and eosin staining. The interleukin (IL)‑1β, IL‑6, and tumor necrosis factor‑α levels in the ascitic fluid, serum, and ileum tissues were evaluated using an enzyme‑linked immunosorbent assay (ELISA). Tight junction proteins, zonula occludens‑1 (ZO‑1) and occludin, in ileum tissues were studied using immunofluorescence. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (CRE) and urea levels were measured using an automatic biochemical analyzer. The results of the present study indicated that both 3.5 and 5% NaT could induce a stable elevation of pancreatitis indices, with histopathological injury of the pancreas, lungs and ileum (5% NaT). The ascitic fluid levels of IL‑6 and IL‑1β were increased in the 5% NaT group. ALT and AST levels increased temporarily and recovered in 72 h, without a significant increase in CRE and urea levels or apparent hepatic and renal pathological injury. In conclusion, rats with NaT‑induced SAP have characteristics of necrotizing hemorrhagic pancreatitis with multiple organ injuries, including inflammatory lung injury, ischemic intestinal injury and slight liver and kidney injuries.

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