Open Access

Upregulation of osteoprotegerin inhibits tert‑butyl hydroperoxide‑induced apoptosis of human chondrocytes

  • Authors:
    • Qifeng Ren
    • Wenfei Zhang
    • Ping Li
    • Jianli Zhou
    • Zhonghao Li
    • Yang Zhou
    • Ming Li
  • View Affiliations

  • Published online on: May 26, 2022     https://doi.org/10.3892/etm.2022.11397
  • Article Number: 470
  • Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

Necrosis of the femoral head (NFH) is an orthopedic disease characterized by a severe lack of blood supply to the femoral head and a marked increase in intraosseous pressure. NFH is associated with numerous factors, such as alcohol consumption and hormone levels. The present study focused on the expression levels of osteoprotegerin (OPG) in NFH and the effect of OPG overexpression on chondrocyte apoptosis. The results demonstrated that OPG expression was markedly decreased in the femoral head of patients with NFH compared with normal femoral heads. Lentivirus‑mediated overexpression of OPG in human chondrocytes reversed the decrease in cell viability and the increase in reactive oxygen species production induced by an oxidative stress‑inducing factor, tert‑butyl hydroperoxide. Flow cytometry and TUNEL assays revealed that OPG overexpression inhibited the apoptosis of chondrocytes. In addition, it was revealed that OPG exerted its anti‑apoptotic effect mainly by promoting Bcl‑2 expression and Akt phosphorylation and inhibiting caspase‑3 cleavage and Bax expression. The present study revealed that OPG may be an important regulator of NFH.
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July-2022
Volume 24 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ren Q, Zhang W, Li P, Zhou J, Li Z, Zhou Y and Li M: Upregulation of osteoprotegerin inhibits <em>tert</em>‑butyl hydroperoxide‑induced apoptosis of human chondrocytes. Exp Ther Med 24: 470, 2022
APA
Ren, Q., Zhang, W., Li, P., Zhou, J., Li, Z., Zhou, Y., & Li, M. (2022). Upregulation of osteoprotegerin inhibits <em>tert</em>‑butyl hydroperoxide‑induced apoptosis of human chondrocytes. Experimental and Therapeutic Medicine, 24, 470. https://doi.org/10.3892/etm.2022.11397
MLA
Ren, Q., Zhang, W., Li, P., Zhou, J., Li, Z., Zhou, Y., Li, M."Upregulation of osteoprotegerin inhibits <em>tert</em>‑butyl hydroperoxide‑induced apoptosis of human chondrocytes". Experimental and Therapeutic Medicine 24.1 (2022): 470.
Chicago
Ren, Q., Zhang, W., Li, P., Zhou, J., Li, Z., Zhou, Y., Li, M."Upregulation of osteoprotegerin inhibits <em>tert</em>‑butyl hydroperoxide‑induced apoptosis of human chondrocytes". Experimental and Therapeutic Medicine 24, no. 1 (2022): 470. https://doi.org/10.3892/etm.2022.11397