Applications of polymerase chain reaction‑based methods for the diagnosis of plague (Review)

  • Authors:
    • Yanan Zhang
    • Zhanli Wang
    • Wenrui Wang
    • Hui Yu
    • Min Jin
  • View Affiliations

  • Published online on: June 14, 2022     https://doi.org/10.3892/etm.2022.11438
  • Article Number: 511
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Abstract

Plague is an acute bacterial infection caused by Yersinia pestis. The three major clinical forms of plague are bubonic, pneumonic and septicemic, which have high case‑mortality rates. Therefore, rapid and reliable diagnostic tools are crucial. Currently, bacteriological means and traditional serological assays are used for detecting infection with Y. pestis. However, such methods have their limitations. Polymerase chain reaction (PCR) is one of the most useful tools for rapid diagnosis of plague. The present review introduced the main PCR techniques and their applications for detecting and confirmation of Y. pestis. The advantages and disadvantages of the different PCR methods were also summarized.
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August-2022
Volume 24 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang Y, Wang Z, Wang W, Yu H and Jin M: Applications of polymerase chain reaction‑based methods for the diagnosis of plague (Review). Exp Ther Med 24: 511, 2022
APA
Zhang, Y., Wang, Z., Wang, W., Yu, H., & Jin, M. (2022). Applications of polymerase chain reaction‑based methods for the diagnosis of plague (Review). Experimental and Therapeutic Medicine, 24, 511. https://doi.org/10.3892/etm.2022.11438
MLA
Zhang, Y., Wang, Z., Wang, W., Yu, H., Jin, M."Applications of polymerase chain reaction‑based methods for the diagnosis of plague (Review)". Experimental and Therapeutic Medicine 24.2 (2022): 511.
Chicago
Zhang, Y., Wang, Z., Wang, W., Yu, H., Jin, M."Applications of polymerase chain reaction‑based methods for the diagnosis of plague (Review)". Experimental and Therapeutic Medicine 24, no. 2 (2022): 511. https://doi.org/10.3892/etm.2022.11438