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Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats

  • Authors:
    • Miao Zhang
    • Yunzhe Sun
    • Chunjie Ding
    • Shan Hong
    • Ning Li
    • Yi Guan
    • Lin Zhang
    • Xinwen Dong
    • Jia Cao
    • Wu Yao
    • Wenjie Ren
    • Sanqiao Yao
  • View Affiliations / Copyright

    Affiliations: Research Center for Precision Prevention and Control of Occupational Hazards, School of Public Health, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Occupational and Environmental Health, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China, Key Laboratory of Birth Regulation and Control Technology, National Health Commission of China, Maternal and Child Care Hospital of Shandong Province, Shandong University, Jinan, Shandong 250001, P.R. China, Institute of Toxicology, College of Preventive Medicine, Army Medical University, Chongqing 400038, P.R. China, Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 529
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    Published online on: June 20, 2022
       https://doi.org/10.3892/etm.2022.11456
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Abstract

Gas explosions are a recurrent event in coal mining that cause severe pulmonary damage due to shock waves, and there is currently no effective targeted treatment. To illustrate the mechanism of gas explosion‑induced lung injury and to explore strategies for blast lung injury (BLI) treatment, the present study used a BLI rat model and supplementation with metformin (MET), an AMP‑activated protein kinase (AMPK) activator, at a dose of 10 mg/kg body weight by intraperitoneal injection. Protein expression levels were detected by western blotting. Significantly decreased expression of phosphorylated (p)‑AMPK, peroxisome proliferator‑activated receptor‑γ coactivator‑1α (PGC1α) and metabolic activity were observed in the BLI group compared with those in the control group. However, the mitochondrial stability, metabolic activity and expression of p‑AMPK and PGC1α were elevated following MET treatment. These results suggested that MET could attenuate gas explosion‑induced BLI by improving mitochondrial homeostasis. Meanwhile, high expression of nicotinamide adenine dinucleotide phosphate oxidase (NOX2) and low expression of catalase (CAT) were observed in the BLI group. The expression levels of NOX2 and CAT were restored in the BLI + MET group relative to changes in the BLI group, and the accumulation of oxidative stress was successfully reversed following MET treatment. Overall, these findings revealed that MET could alleviate BLI by activating the AMPK/PGC1α pathway and inhibiting oxidative stress caused by NOX2 activation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang M, Sun Y, Ding C, Hong S, Li N, Guan Y, Zhang L, Dong X, Cao J, Yao W, Yao W, et al: Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats. Exp Ther Med 24: 529, 2022.
APA
Zhang, M., Sun, Y., Ding, C., Hong, S., Li, N., Guan, Y. ... Yao, S. (2022). Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats. Experimental and Therapeutic Medicine, 24, 529. https://doi.org/10.3892/etm.2022.11456
MLA
Zhang, M., Sun, Y., Ding, C., Hong, S., Li, N., Guan, Y., Zhang, L., Dong, X., Cao, J., Yao, W., Ren, W., Yao, S."Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats". Experimental and Therapeutic Medicine 24.2 (2022): 529.
Chicago
Zhang, M., Sun, Y., Ding, C., Hong, S., Li, N., Guan, Y., Zhang, L., Dong, X., Cao, J., Yao, W., Ren, W., Yao, S."Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats". Experimental and Therapeutic Medicine 24, no. 2 (2022): 529. https://doi.org/10.3892/etm.2022.11456
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang M, Sun Y, Ding C, Hong S, Li N, Guan Y, Zhang L, Dong X, Cao J, Yao W, Yao W, et al: Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats. Exp Ther Med 24: 529, 2022.
APA
Zhang, M., Sun, Y., Ding, C., Hong, S., Li, N., Guan, Y. ... Yao, S. (2022). Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats. Experimental and Therapeutic Medicine, 24, 529. https://doi.org/10.3892/etm.2022.11456
MLA
Zhang, M., Sun, Y., Ding, C., Hong, S., Li, N., Guan, Y., Zhang, L., Dong, X., Cao, J., Yao, W., Ren, W., Yao, S."Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats". Experimental and Therapeutic Medicine 24.2 (2022): 529.
Chicago
Zhang, M., Sun, Y., Ding, C., Hong, S., Li, N., Guan, Y., Zhang, L., Dong, X., Cao, J., Yao, W., Ren, W., Yao, S."Metformin mitigates gas explosion‑induced blast lung injuries through AMPK‑mediated energy metabolism and NOX2‑related oxidation pathway in rats". Experimental and Therapeutic Medicine 24, no. 2 (2022): 529. https://doi.org/10.3892/etm.2022.11456
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