Open Access

Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer

  • Authors:
    • Won Hyeok Lee
    • Song Hee Kim
    • Jae Hee An
    • Tae-Koon Kim
    • Hee Jeong Cha
    • Hyo Won Chang
    • Sang Yoon Kim
    • Seong Who Kim
    • Myung Woul Han
  • View Affiliations

  • Published online on: June 29, 2022     https://doi.org/10.3892/etm.2022.11478
  • Article Number: 541
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

CD47 is expressed in all human cancer cells, including head and neck cancer, and initiates a signaling cascade to inhibit macrophage phagocytosis. However, the mecha­nism underlying CD47 overexpression has not been elucidated in radioresistant head and neck cancer. The present study demonstrated that decreased Tristetraprolin (TTP) expression induced a sustained overexpression of CD47 using reverse transcription‑quantitative PCR and western blotting, and that CD47 overexpression prevented phagocytosis using a phagocytosis assay in a radioresistant HN31R cell line. Subsequently, using TTP transfection, RNA interference, duel‑luciferase assay and EMSA, it was revealed that TTP transfection enhanced phagocytosis through degradation of CD47 mRNA by directly binding to CD47 AREs within the CD47 3'UTR. Based on our previous study, methylation‑specific PCR and western blotting revealed that DNMT1 was overexpressed in radioresistant HN31R cell line and TTP expression was decreased epigenetically by DMNT1 associated DNA methylation. Overall, these findings provided novel insight into the role of TTP as a biomarker of CD47‑positive head and neck cancer patients.

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September-2022
Volume 24 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lee WH, Kim SH, An JH, Kim T, Cha HJ, Chang HW, Kim SY, Kim SW and Han MW: Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer. Exp Ther Med 24: 541, 2022
APA
Lee, W.H., Kim, S.H., An, J.H., Kim, T., Cha, H.J., Chang, H.W. ... Han, M.W. (2022). Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer. Experimental and Therapeutic Medicine, 24, 541. https://doi.org/10.3892/etm.2022.11478
MLA
Lee, W. H., Kim, S. H., An, J. H., Kim, T., Cha, H. J., Chang, H. W., Kim, S. Y., Kim, S. W., Han, M. W."Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer". Experimental and Therapeutic Medicine 24.3 (2022): 541.
Chicago
Lee, W. H., Kim, S. H., An, J. H., Kim, T., Cha, H. J., Chang, H. W., Kim, S. Y., Kim, S. W., Han, M. W."Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer". Experimental and Therapeutic Medicine 24, no. 3 (2022): 541. https://doi.org/10.3892/etm.2022.11478