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Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer

  • Authors:
    • Won Hyeok Lee
    • Song Hee Kim
    • Jae Hee An
    • Tae-Koon Kim
    • Hee Jeong Cha
    • Hyo Won Chang
    • Sang Yoon Kim
    • Seong Who Kim
    • Myung Woul Han
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 44033, Republic of Korea, Department of Pathology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 44033, Republic of Korea, Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea, Department of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 541
    |
    Published online on: June 29, 2022
       https://doi.org/10.3892/etm.2022.11478
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Abstract

CD47 is expressed in all human cancer cells, including head and neck cancer, and initiates a signaling cascade to inhibit macrophage phagocytosis. However, the mecha­nism underlying CD47 overexpression has not been elucidated in radioresistant head and neck cancer. The present study demonstrated that decreased Tristetraprolin (TTP) expression induced a sustained overexpression of CD47 using reverse transcription‑quantitative PCR and western blotting, and that CD47 overexpression prevented phagocytosis using a phagocytosis assay in a radioresistant HN31R cell line. Subsequently, using TTP transfection, RNA interference, duel‑luciferase assay and EMSA, it was revealed that TTP transfection enhanced phagocytosis through degradation of CD47 mRNA by directly binding to CD47 AREs within the CD47 3'UTR. Based on our previous study, methylation‑specific PCR and western blotting revealed that DNMT1 was overexpressed in radioresistant HN31R cell line and TTP expression was decreased epigenetically by DMNT1 associated DNA methylation. Overall, these findings provided novel insight into the role of TTP as a biomarker of CD47‑positive head and neck cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Lee WH, Kim SH, An JH, Kim T, Cha HJ, Chang HW, Kim SY, Kim SW and Han MW: Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer. Exp Ther Med 24: 541, 2022.
APA
Lee, W.H., Kim, S.H., An, J.H., Kim, T., Cha, H.J., Chang, H.W. ... Han, M.W. (2022). Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer. Experimental and Therapeutic Medicine, 24, 541. https://doi.org/10.3892/etm.2022.11478
MLA
Lee, W. H., Kim, S. H., An, J. H., Kim, T., Cha, H. J., Chang, H. W., Kim, S. Y., Kim, S. W., Han, M. W."Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer". Experimental and Therapeutic Medicine 24.3 (2022): 541.
Chicago
Lee, W. H., Kim, S. H., An, J. H., Kim, T., Cha, H. J., Chang, H. W., Kim, S. Y., Kim, S. W., Han, M. W."Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer". Experimental and Therapeutic Medicine 24, no. 3 (2022): 541. https://doi.org/10.3892/etm.2022.11478
Copy and paste a formatted citation
x
Spandidos Publications style
Lee WH, Kim SH, An JH, Kim T, Cha HJ, Chang HW, Kim SY, Kim SW and Han MW: Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer. Exp Ther Med 24: 541, 2022.
APA
Lee, W.H., Kim, S.H., An, J.H., Kim, T., Cha, H.J., Chang, H.W. ... Han, M.W. (2022). Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer. Experimental and Therapeutic Medicine, 24, 541. https://doi.org/10.3892/etm.2022.11478
MLA
Lee, W. H., Kim, S. H., An, J. H., Kim, T., Cha, H. J., Chang, H. W., Kim, S. Y., Kim, S. W., Han, M. W."Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer". Experimental and Therapeutic Medicine 24.3 (2022): 541.
Chicago
Lee, W. H., Kim, S. H., An, J. H., Kim, T., Cha, H. J., Chang, H. W., Kim, S. Y., Kim, S. W., Han, M. W."Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer". Experimental and Therapeutic Medicine 24, no. 3 (2022): 541. https://doi.org/10.3892/etm.2022.11478
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