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Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report

  • Authors:
    • Rong Wang
    • Rong Feng
    • Chaoran Xia
    • Fangying Ruan
    • Peng Luo
    • Jun Guo
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    Affiliations: Department of Critical Medicine, Union Jiangbei Hospital, Huazhong University of Science and Technology, Wuhan, Hubei 430100, P.R. China, Shanghai Topgen Biomedical Technology Co., Ltd., Shanghai 201318, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 573
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    Published online on: July 15, 2022
       https://doi.org/10.3892/etm.2022.11510
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Abstract

Metagenomic next‑generation sequencing (mNGS) is an effective method that can be used for the identification of early pathogens in patients with suspected severe pneumonia. However, the potential of mNGS for evaluating the prognosis of acute respiratory distress syndrome (ARDS) in patients with severe pneumonia remains unclear. In the present report, hospital‑acquired gram‑negative bacteria infections were detected in a case using metagenomic next‑generation sequencing (mNGS) in a sample of bronchoalveolar fluid. This was obtained from a 58‑year‑old male patient with traumatic wet lung after a neurosurgery. According to the results, of which the profiles of the resistance genes were detected by mNGS, drugs designed to control infection were adjusted, namely to polymyxin B (500,000 U/12 h), azithromycin (0.5 g/24 h) and ganciclovir (0.25 g/12 h). Following adjusting treatment for 8 days, the symptoms of lung infection and hypoxemia were markedly improved, resulting in the patient being transferred out of the intensive care unit 15 days after treatment. To conclude, observations from the present report suggest that mNGS is a useful method for the early identification of pathogens in patients with pneumonia caused by ARDS. However, further studies are required to identify the complementary role of mNGS in supporting conventional microbiological methods in routine clinical practice.
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Copy and paste a formatted citation
Spandidos Publications style
Wang R, Feng R, Xia C, Ruan F, Luo P and Guo J: Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report. Exp Ther Med 24: 573, 2022.
APA
Wang, R., Feng, R., Xia, C., Ruan, F., Luo, P., & Guo, J. (2022). Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report. Experimental and Therapeutic Medicine, 24, 573. https://doi.org/10.3892/etm.2022.11510
MLA
Wang, R., Feng, R., Xia, C., Ruan, F., Luo, P., Guo, J."Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report". Experimental and Therapeutic Medicine 24.3 (2022): 573.
Chicago
Wang, R., Feng, R., Xia, C., Ruan, F., Luo, P., Guo, J."Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report". Experimental and Therapeutic Medicine 24, no. 3 (2022): 573. https://doi.org/10.3892/etm.2022.11510
Copy and paste a formatted citation
x
Spandidos Publications style
Wang R, Feng R, Xia C, Ruan F, Luo P and Guo J: Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report. Exp Ther Med 24: 573, 2022.
APA
Wang, R., Feng, R., Xia, C., Ruan, F., Luo, P., & Guo, J. (2022). Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report. Experimental and Therapeutic Medicine, 24, 573. https://doi.org/10.3892/etm.2022.11510
MLA
Wang, R., Feng, R., Xia, C., Ruan, F., Luo, P., Guo, J."Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report". Experimental and Therapeutic Medicine 24.3 (2022): 573.
Chicago
Wang, R., Feng, R., Xia, C., Ruan, F., Luo, P., Guo, J."Early detection of gram‑negative bacteria using metagenomic next‑generation sequencing in acute respiratory distress syndrome: A case report". Experimental and Therapeutic Medicine 24, no. 3 (2022): 573. https://doi.org/10.3892/etm.2022.11510
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