Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2022 Volume 24 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2022 Volume 24 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Combination of azithromycin and methylprednisolone alleviates Mycoplasma pneumoniae induced pneumonia by regulating miR‑499a‑5p/STAT3 axis

  • Authors:
    • Yongli Chen
    • Shanwu Dong
    • Lin Tian
    • Haishan Chen
    • Jing Chen
    • Chunzhi He
  • View Affiliations / Copyright

    Affiliations: Department of Paediatrics, Wuhan Fourth Hospital, Hankou, Wuhan, Hubei 430034, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 578
    |
    Published online on: July 19, 2022
       https://doi.org/10.3892/etm.2022.11515
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Mycoplasma pneumoniae (M. pneumoniae) is a contributing factor to community‑acquired pneumonia in children. The present study sought to explain the underlying mechanism of azithromycin (AZM) combined with methylprednisolone (MP) in the treatment of M. pneumoniae infection. Peripheral blood samples were obtained from patients with M. pneumoniae and healthy volunteers for analysis. A549 cells were infected with M. pneumoniae to construct an in vitro cell model with M. pneumoniae, followed by treatment with AZM and MP. Cell Counting Kit‑8 and TUNEL assays were conducted to detect cell viability and apoptosis. RT‑qPCR was employed to measure the expression levels of microRNA (miR)‑499a‑5p and STAT3. Western blotting was performed to measure the expression of STAT3 and apoptosis‑related proteins. Luciferase report assay was performed to verify the binding site between miR‑499a‑5p and STAT3. The production of inflammatory cytokines was determined using ELISA kits. The results exhibited the downregulated miR‑499a‑5p and dysregulated inflammatory cytokines in peripheral blood of patients and M. pneumoniae‑infected A549 cells. AZM and MP treatment alone or combined significantly inhibited inflammatory response, cell viability loss and promoted apoptosis in A549 cells infected with M. pneumoniae, which was partly reversed by inhibition of miR‑499a‑5p. Furthermore, miR‑499a‑5p could negatively regulate its direct target STAT3. In addition, STAT3 is also regulated by AZM and MP. Collectively, the present results suggested that combination treatment of AZM and MP could inhibit M. pneumoniae infection‑induced inflammation, cell viability loss and promoted apoptosis partly by regulating miR‑499a‑5p/STAT3 axis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Wang X, Chen X, Tang H, Zhu J, Zhou S, Xu Z, Liu F and Su C: Increased frequency of Th17 cells in children with Mycoplasma pneumoniae pneumonia. J Clin Lab Anal. 30:1214–1219. 2016.PubMed/NCBI View Article : Google Scholar

2 

Atkinson TP and Waites KB: Mycoplasma pneumoniae infections in childhood. Pediatr Infect Dis J. 33:92–94. 2014.PubMed/NCBI View Article : Google Scholar

3 

Cartner SC, Lindsey JR, Gibbs-Erwin J, Cassell GH and Simecka JW: Roles of innate and adaptive immunity in respiratory mycoplasmosis. Infect Immun. 66:3485–3491. 1998.PubMed/NCBI View Article : Google Scholar

4 

Waites KB and Talkington DF: Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev. 17:697–728. 2004.PubMed/NCBI View Article : Google Scholar

5 

Ferwerda A, Moll HA and de Groot R: Respiratory tract infections by Mycoplasma pneumoniae in children: A review of diagnostic and therapeutic measures. Eur J Pediatr. 160:483–491. 2001.PubMed/NCBI View Article : Google Scholar

6 

Gao S, Wang L, Zhu W and Jiang J: Mycoplasma pneumonia infection and asthma: A clinical study. Pak J Med Sci. 31:548–551. 2015.PubMed/NCBI View Article : Google Scholar

7 

Spiga F, Zhao Z and Lightman SL: Prolonged treatment with the synthetic glucocorticoid methylprednisolone affects adrenal steroidogenic function and response to inflammatory stress in the rat. Brain Behav Immun. 87:703–714. 2020.PubMed/NCBI View Article : Google Scholar

8 

Shan LS, Liu X, Kang XY, Wang F, Han XH and Shang YX: Effects of methylprednisolone or immunoglobulin when added to standard treatment with intravenous azithromycin for refractory Mycoplasma pneumoniae pneumonia in children. World J Pediatr. 13:321–327. 2017.PubMed/NCBI View Article : Google Scholar

9 

Pu M, Chen J, Tao Z, Miao L, Qi X, Wang Y and Ren J: Regulatory network of miRNA on its target: coordination between transcriptional and post-transcriptional regulation of gene expression. Cell Mol Life Sci. 76:441–451. 2019.PubMed/NCBI View Article : Google Scholar

10 

Ebrahimi A and Sadroddiny E: MicroRNAs in lung diseases: Recent findings and their pathophysiological implications. Pulm Pharmacol Ther. 34:55–63. 2015.PubMed/NCBI View Article : Google Scholar

11 

Zhao L, Wang B, Zhang W and Sun L: Effect of miR-499a-5p on damage of cardiomyocyte induced by hypoxia-reoxygenation via downregulating CD38 protein. J Cell Biochem. 121:996–1004. 2020.PubMed/NCBI View Article : Google Scholar

12 

He S, Li Z, Yu Y, Zeng Q, Cheng Y, Ji W, Xia W and Lu S: Exosomal miR-499a-5p promotes cell proliferation, migration and EMT via mTOR signaling pathway in lung adenocarcinoma. Exp Cell Res. 379:203–213. 2019.PubMed/NCBI View Article : Google Scholar

13 

Wang M, Yang C, Liu X, Zheng J, Xue Y, Ruan X, Shen S, Wang D, Li Z, Cai H and Liu Y: An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1-AS1/miR-499a-5p/ELF1/EMI1 pathway. J Cell Mol Med. 24:6120–6136. 2020.PubMed/NCBI View Article : Google Scholar

14 

Ozkul Y, Taheri S, Bayram KK, Sener EF, Mehmetbeyoglu E, Öztop DB, Aybuga F, Tufan E, Bayram A, Dolu N, et al: A heritable profile of six miRNAs in autistic patients and mouse models. Sci Rep. 10(9011)2020.PubMed/NCBI View Article : Google Scholar

15 

Singh AK, Rooge SB, Varshney A, Vasudevan M, Bhardwaj A, Venugopal SK, Trehanpati N, Kumar M, Geffers R, Kumar V and Sarin SK: Global microRNA expression profiling in the liver biopsies of hepatitis B virus-infected patients suggests specific microRNA signatures for viral persistence and hepatocellular injury. Hepatology. 67:1695–1709. 2018.PubMed/NCBI View Article : Google Scholar

16 

Zhang W, Li J, Yao H and Li T: Restoring microRNA-499-5p protects sepsis-induced lung injury mice via targeting Sox6. Nanoscale Res Lett. 16(89)2021.PubMed/NCBI View Article : Google Scholar

17 

Jarret A, McFarland AP, Horner SM, Kell A, Schwerk J, Hong M, Badil S, Joslyn RC, Baker DP, Carrington M, et al: Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling. Nat Med. 22:1475–1481. 2016.PubMed/NCBI View Article : Google Scholar

18 

Lin Y, Tan D, Kan Q, Xiao Z and Jiang Z: The protective effect of Naringenin on airway remodeling after Mycoplasma pneumoniae infection by inhibiting autophagy-mediated lung inflammation and fibrosis. Mediators Inflamm. 2018(8753894)2018.PubMed/NCBI View Article : Google Scholar

19 

Liu F, Zhang X, Zhang B, Mao W, Liu T, Sun M and Wu Y: TREM1: A positive regulator for inflammatory response via NF-κB pathway in A549 cells infected with Mycoplasma pneumoniae. Biomed Pharmacother. 107:1466–1472. 2018.PubMed/NCBI View Article : Google Scholar

20 

Zerin T, Kim YS, Hong SY and Song HY: Protective effect of methylprednisolone on paraquat-induced A549 cell cytotoxicity via induction of efflux transporter, P-glycoprotein expression. Toxicol Lett. 208:101–107. 2012.PubMed/NCBI View Article : Google Scholar

21 

Kitsiouli E, Antoniou G, Gotzou H, Karagiannopoulos M, Basagiannis D, Christoforidis S, Nakos G and Lekka ME: Effect of azithromycin on the LPS-induced production and secretion of phospholipase A2 in lung cells. Biochim Biophys Acta. 1852:1288–1297. 2015.PubMed/NCBI View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

23 

Shi S, Liu X and Li H: Downregulation of caspase3 alleviates Mycoplasma pneumoniae induced apoptosis in alveolar epithelial cells. Mol Med Rep. 16:9601–9606. 2017.PubMed/NCBI View Article : Google Scholar

24 

Segovia JA, Chang TH, Winter VT, Coalson JJ, Cagle MP, Pandranki L, Bose S, Baseman JB and Kannan TR: NLRP3 is a critical regulator of inflammation and innate immune cell response during Mycoplasma pneumoniae infection. Infect Immun. 86:e00548–17. 2018.PubMed/NCBI View Article : Google Scholar

25 

Liu LQ, Wang ZH and Yao HY: Hepatocyte growth factor can guide treatment of Mycoplasma pneumoniae pneumonia in children. Exp Ther Med. 19:3432–3438. 2020.PubMed/NCBI View Article : Google Scholar

26 

Zhang C, Dong WB, Zhao S, Li QP, Kang L, Lei XP, Guo L and Zhai XS: Construction of p66Shc gene interfering lentivirus vectors and its effects on alveolar epithelial cells apoptosis induced by hyperoxia. Drug Des Devel Ther. 10:2611–2622. 2016.PubMed/NCBI View Article : Google Scholar

27 

Sun G, Xu X, Wang Y, Shen X, Chen Z and Yang J: Mycoplasma pneumoniae infection induces reactive oxygen species and DNA damage in A549 human lung carcinoma cells. Infect Immun. 76:4405–4413. 2008.PubMed/NCBI View Article : Google Scholar

28 

Gu X, Dong M, Liu Z, Yang J and Shi Y: MiR-499a-5p inhibits proliferation, invasion, migration, and epithelial-mesenchymal transition, and enhances radiosensitivity of cervical cancer cells via targeting eIF4E. Onco Targets Ther. 13:2913–2924. 2020.PubMed/NCBI View Article : Google Scholar

29 

Guanizo AC, Fernando CD, Garama DJ and Gough DJ: STAT3: A multifaceted oncoprotein. Growth Factors. 36:1–14. 2018.PubMed/NCBI View Article : Google Scholar

30 

Deverrière G, Lemée L, Grangé S, Boyer S, Picard C, Fischer A and Marguet C: Life-threatening Pneumopathy and U urealyticum in a STAT3-deficient hyper-IgE syndrome patient. Pediatrics. 139(e20160845)2017.PubMed/NCBI View Article : Google Scholar

31 

Hao Y, Kuang Z, Jing J, Miao J, Mei LY, Lee RJ, Kim S, Choe S, Krause DC and Lau GW: Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins. Infect Immun. 82:5246–5255. 2014.PubMed/NCBI View Article : Google Scholar

32 

Choi SY, Lim JW, Shimizu T, Kuwano K, Kim JM and Kim H: Reactive oxygen species mediate Jak2/Stat3 activation and IL-8 expression in pulmonary epithelial cells stimulated with lipid-associated membrane proteins from Mycoplasma pneumoniae. Inflamm Res. 61:493–501. 2012.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Dong S, Tian L, Chen H, Chen J and He C: Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis. Exp Ther Med 24: 578, 2022.
APA
Chen, Y., Dong, S., Tian, L., Chen, H., Chen, J., & He, C. (2022). Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis. Experimental and Therapeutic Medicine, 24, 578. https://doi.org/10.3892/etm.2022.11515
MLA
Chen, Y., Dong, S., Tian, L., Chen, H., Chen, J., He, C."Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis". Experimental and Therapeutic Medicine 24.3 (2022): 578.
Chicago
Chen, Y., Dong, S., Tian, L., Chen, H., Chen, J., He, C."Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis". Experimental and Therapeutic Medicine 24, no. 3 (2022): 578. https://doi.org/10.3892/etm.2022.11515
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Dong S, Tian L, Chen H, Chen J and He C: Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis. Exp Ther Med 24: 578, 2022.
APA
Chen, Y., Dong, S., Tian, L., Chen, H., Chen, J., & He, C. (2022). Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis. Experimental and Therapeutic Medicine, 24, 578. https://doi.org/10.3892/etm.2022.11515
MLA
Chen, Y., Dong, S., Tian, L., Chen, H., Chen, J., He, C."Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis". Experimental and Therapeutic Medicine 24.3 (2022): 578.
Chicago
Chen, Y., Dong, S., Tian, L., Chen, H., Chen, J., He, C."Combination of azithromycin and methylprednisolone alleviates <em>Mycoplasma pneumoniae</em> induced pneumonia by regulating miR‑499a‑5p/STAT3 axis". Experimental and Therapeutic Medicine 24, no. 3 (2022): 578. https://doi.org/10.3892/etm.2022.11515
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team