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ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway

  • Authors:
    • Xinfeng Qian
    • Linjun Yang
  • View Affiliations

  • Published online on: July 28, 2022     https://doi.org/10.3892/etm.2022.11540
  • Article Number: 603
  • Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rho‑associated protein kinase 2 (ROCK2) is an important regulator of the inflammatory response and has been reported to serve a role in sepsis. The present study aimed to investigate whether ROCK2 served a role in sepsis‑associated acute kidney injury (S‑AKI). HK‑2 cells were stimulated with lipopolysaccharide (LPS) to simulate S‑AKI in vitro. Subsequently, the change in ROCK2 expression levels were determined. ROCK2 in LPS‑induced HK‑2 cells was knocked down using short hairpin RNA‑ROCK2, in the absence or presence of phorbol 12‑myristate 13‑acetate (PMA), an activator of NF‑κB. Cell viability, cytotoxicity, inflammation and apoptosis were assessed using MTT, lactate dehydrogenase (LDH) release, reverse transcription‑quantitative PCR, ELISA, TUNEL and western blotting assays. The protein expression levels of proteins involved in the NF‑κB/NLR family pyrin domain containing 3 (NLRP3) signaling pathway were also assessed using western blotting. The results demonstrated that ROCK2 was upregulated in HK‑2 cells upon LPS treatment. LPS also reduced cell viability, promoted LDH activity and increased TNF‑α, IL‑6 and IL‑1β mRNA expression levels and concentrations. Apoptosis was also induced by LPS as indicated by an increase in the proportion of TUNEL‑positive cells, decreased Bcl‑2 protein expression levels and increased cleaved caspase‑3 and cleaved poly (ADP‑ribose) polymerase protein expression levels. However, ROCK2 knockdown in LPS‑induced HK‑2 cells reversed cell viability damage and inhibited LDH activity, the generation of pro‑inflammatory cytokines and apoptosis caused by LPS. Furthermore, ROCK2 knockdown inhibited the LPS‑induced expression of phosphorylated‑NF‑κB p65, NLRP3, apoptosis‑associated speck‑like protein containing a CARD and caspase‑1 p20. PMA treatment reversed all the aforementioned effects of ROCK2 knockdown on LPS‑treated HK‑2 cells. Therefore, ROCK2 knockdown may alleviate LPS‑induced HK‑2 cell injury via the inactivation of the NF‑κB/NLRP3 signaling pathway.

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September-2022
Volume 24 Issue 3

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Spandidos Publications style
Qian X and Qian X: ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway. Exp Ther Med 24: 603, 2022
APA
Qian, X., & Qian, X. (2022). ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway. Experimental and Therapeutic Medicine, 24, 603. https://doi.org/10.3892/etm.2022.11540
MLA
Qian, X., Yang, L."ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway". Experimental and Therapeutic Medicine 24.3 (2022): 603.
Chicago
Qian, X., Yang, L."ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway". Experimental and Therapeutic Medicine 24, no. 3 (2022): 603. https://doi.org/10.3892/etm.2022.11540