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ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway

  • Authors:
    • Xinfeng Qian
    • Linjun Yang
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    Affiliations: Department of Emergency, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214000, P.R. China, Department of Emergency, Hanzhong People's Hospital, Hanzhong, Shaanxi 723000, P.R. China
    Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 603
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    Published online on: July 28, 2022
       https://doi.org/10.3892/etm.2022.11540
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Abstract

Rho‑associated protein kinase 2 (ROCK2) is an important regulator of the inflammatory response and has been reported to serve a role in sepsis. The present study aimed to investigate whether ROCK2 served a role in sepsis‑associated acute kidney injury (S‑AKI). HK‑2 cells were stimulated with lipopolysaccharide (LPS) to simulate S‑AKI in vitro. Subsequently, the change in ROCK2 expression levels were determined. ROCK2 in LPS‑induced HK‑2 cells was knocked down using short hairpin RNA‑ROCK2, in the absence or presence of phorbol 12‑myristate 13‑acetate (PMA), an activator of NF‑κB. Cell viability, cytotoxicity, inflammation and apoptosis were assessed using MTT, lactate dehydrogenase (LDH) release, reverse transcription‑quantitative PCR, ELISA, TUNEL and western blotting assays. The protein expression levels of proteins involved in the NF‑κB/NLR family pyrin domain containing 3 (NLRP3) signaling pathway were also assessed using western blotting. The results demonstrated that ROCK2 was upregulated in HK‑2 cells upon LPS treatment. LPS also reduced cell viability, promoted LDH activity and increased TNF‑α, IL‑6 and IL‑1β mRNA expression levels and concentrations. Apoptosis was also induced by LPS as indicated by an increase in the proportion of TUNEL‑positive cells, decreased Bcl‑2 protein expression levels and increased cleaved caspase‑3 and cleaved poly (ADP‑ribose) polymerase protein expression levels. However, ROCK2 knockdown in LPS‑induced HK‑2 cells reversed cell viability damage and inhibited LDH activity, the generation of pro‑inflammatory cytokines and apoptosis caused by LPS. Furthermore, ROCK2 knockdown inhibited the LPS‑induced expression of phosphorylated‑NF‑κB p65, NLRP3, apoptosis‑associated speck‑like protein containing a CARD and caspase‑1 p20. PMA treatment reversed all the aforementioned effects of ROCK2 knockdown on LPS‑treated HK‑2 cells. Therefore, ROCK2 knockdown may alleviate LPS‑induced HK‑2 cell injury via the inactivation of the NF‑κB/NLRP3 signaling pathway.
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1 

Bellomo R, Kellum JA and Ronco C: Acute kidney injury. Lancet. 380:756–766. 2012.PubMed/NCBI View Article : Google Scholar

2 

Peerapornratana S, Manrique-Caballero CL, Gómez H and Kellum JA: Acute kidney injury from sepsis: Current concepts, epidemiology, pathophysiology, prevention and treatment. Kidney Int. 96:1083–1099. 2019.PubMed/NCBI View Article : Google Scholar

3 

Järvisalo MJ, Hellman T and Uusalo P: Mortality and associated risk factors in patients with blood culture positive sepsis and acute kidney injury requiring continuous renal replacement therapy-A retrospective study. PLoS One. 16(e0249561)2021.PubMed/NCBI View Article : Google Scholar

4 

Poston JT and Koyner JL: Sepsis associated acute kidney injury. BMJ. 364(k4891)2019.PubMed/NCBI View Article : Google Scholar

5 

Lim KH and Staudt LM: Toll-like receptor signaling. Cold Spring Harb Perspect Biol. 5(a011247)2013.PubMed/NCBI View Article : Google Scholar

6 

Qi Y, Liang X, Hu X, He H, Tang L and Yao W: Tetrahydroxystilbene glucoside protects against LPS-induced endothelial dysfunction via inhibiting RhoA/ROCK signaling and F-actin remodeling. Gen Physiol Biophys. 39:407–417. 2020.PubMed/NCBI View Article : Google Scholar

7 

Gao Z, Li Q, Zhang Y, Gao X, Li H and Yuan Z: Ripasudil alleviated the inflammation of RPE cells by targeting the miR-136-5p/ROCK/NLRP3 pathway. BMC Ophthalmol. 20(134)2020.PubMed/NCBI View Article : Google Scholar

8 

Chen H, Hu X, Li R, Liu B, Zheng X, Fang Z, Chen L, Chen W, Min L and Hu S: LncRNA THRIL aggravates sepsis-induced acute lung injury by regulating miR-424/ROCK2 axis. Mol Immunol. 126:111–119. 2020.PubMed/NCBI View Article : Google Scholar

9 

Jianjun Z, Baochun Z, Limei M and Lijun L: Exploring the beneficial role of ROCK inhibitors in sepsis-induced cerebral and cognitive injury in rats. Fundam Clin Pharmacol. 35:882–891. 2021.PubMed/NCBI View Article : Google Scholar

10 

Han J, Ding R, Zhao D, Zhang Z and Ma X: Unfractionated heparin attenuates lung vascular leak in a mouse model of sepsis: Role of RhoA/Rho kinase pathway. Thromb Res. 132:e42–e47. 2013.PubMed/NCBI View Article : Google Scholar

11 

Lawrence T: The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol. 1(a001651)2009.PubMed/NCBI View Article : Google Scholar

12 

Nagai Y, Matoba K, Kawanami D, Takeda Y, Akamine T, Ishizawa S, Kanazawa Y, Yokota T, Utsunomiya K and Nishimura R: ROCK2 regulates TGF-β-induced expression of CTGF and profibrotic genes via NF-κB and cytoskeleton dynamics in mesangial cells. Am J Physiol Renal Physiol. 317:F839–F851. 2019.PubMed/NCBI View Article : Google Scholar

13 

Shi C, Zhao Y, Li Q and Li J: lncRNA SNHG14 plays a role in sepsis-induced acute kidney injury by regulating miR-93. Mediators Inflamm. 2021(5318369)2021.PubMed/NCBI View Article : Google Scholar

14 

Xin F, Wang H, Yuan F and Ding Y: Platelet-rich plasma combined with alendronate reduces pain and inflammation in induced osteoarthritis in rats by inhibiting the nuclear factor-kappa B signaling pathway. Biomed Res Int. 2020(8070295)2020.PubMed/NCBI View Article : Google Scholar

15 

Chen H, Lin W, Lin P, Zheng M, Lai Y, Chen M, Zhang Y, Chen J, Lin X, Lin L, et al: IL-10 produces a dual effect on OGD-induced neuronal apoptosis of cultured cortical neurons via the NF-κB pathway. Aging (Albany NY). 11:10796–10813. 2019.PubMed/NCBI View Article : Google Scholar

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

17 

Krishnamurthy S, Narayanan P, Prabha S, Mondal N, Mahadevan S, Biswal N and Srinivasan S: Clinical profile of acute kidney injury in a pediatric intensive care unit from Southern India: A prospective observational study. Indian J Crit Care Med. 17:207–213. 2013.PubMed/NCBI View Article : Google Scholar

18 

Meyer-Schwesinger C, Dehde S, von Ruffer C, Gatzemeier S, Klug P, Wenzel UO, Stahl RA, Thaiss F and Meyer TN: Rho kinase inhibition attenuates LPS-induced renal failure in mice in part by attenuation of NF-kappaB p65 signaling. Am J Physiol Renal Physiol. 296:F1088–F1099. 2009.PubMed/NCBI View Article : Google Scholar

19 

Nozaki Y, Kinoshita K, Hino S, Yano T, Niki K, Hirooka Y, Kishimoto K, Funauchi M and Matsumura I: Signaling Rho-kinase mediates inflammation and apoptosis in T cells and renal tubules in cisplatin nephrotoxicity. Am J Physiol Renal Physiol. 308:F899–F909. 2015.PubMed/NCBI View Article : Google Scholar

20 

Dickson K and Lehmann C: Inflammatory response to different toxins in experimental sepsis models. Int J Mol Sci. 20(4341)2019.PubMed/NCBI View Article : Google Scholar

21 

Yang J, Ruan F and Zheng Z: Ripasudil attenuates lipopolysaccharide (LPS)-mediated apoptosis and inflammation in pulmonary microvascular endothelial cells via ROCK2/eNOS signaling. Med Sci Monit. 24:3212–3219. 2018.PubMed/NCBI View Article : Google Scholar

22 

Feng S, Zou L, Wang H, He R, Liu K and Zhu H: RhoA/ROCK-2 pathway inhibition and tight junction protein upregulation by catalpol suppresses lipopolysaccaride-induced disruption of blood-brain barrier permeability. Molecules. 23(2371)2018.PubMed/NCBI View Article : Google Scholar

23 

Yan XX, Zheng AD, Zhang ZE, Pan GC and Zhou W: Protective effect of pantoprazole against sepsis-induced acute lung and kidney injury in rats. Am J Transl Res. 11:5197–5211. 2019.PubMed/NCBI

24 

Chen T, Guo Q, Wang H, Zhang H, Wang C, Zhang P, Meng S, Li Y, Ji H and Yan T: Effects of esculetin on lipopolysaccharide (LPS)-induced acute lung injury via regulation of RhoA/Rho kinase/NF-кB pathways in vivo and in vitro. Free Radic Res. 49:1459–1468. 2015.PubMed/NCBI View Article : Google Scholar

25 

Wei F, Liu S, Luo L, Gu N, Zeng Y, Chen X, Xu S and Zhang D: Anti-inflammatory mechanism of ulinastatin: Inhibiting the hyperpermeability of vascular endothelial cells induced by TNF-α via the RhoA/ROCK signal pathway. Int Immunopharmacol. 46:220–227. 2017.PubMed/NCBI View Article : Google Scholar

26 

Liu P, Xiao Z, Yan H, Lu X, Zhang X, Luo L, Long C and Zhu Y: Baicalin suppresses Th1 and Th17 responses and promotes Treg response to ameliorate sepsis-associated pancreatic injury via the RhoA-ROCK pathway. Int Immunopharmacol. 86(106685)2020.PubMed/NCBI View Article : Google Scholar

27 

Ozkok A and Edelstein CL: Pathophysiology of cisplatin-induced acute kidney injury. Biomed Res Int. 2014(967826)2014.PubMed/NCBI View Article : Google Scholar

28 

Li X, Tong J, Liu J and Wang Y: Down-regulation of ROCK2 alleviates ethanol-induced cerebral nerve injury partly by the suppression of the NF-κB signaling pathway. Bioengineered. 11:779–790. 2020.PubMed/NCBI View Article : Google Scholar

29 

Wang Q, Ou Y, Hu G, Wen C, Yue S, Chen C, Xu L, Xie J, Dai H, Xiao H, Zhang Y and Qi R: Naringenin attenuates non-alcoholic fatty liver disease by down-regulating the NLRP3/NF-κB pathway in mice. Br J Pharmacol. 177:1806–1821. 2020.PubMed/NCBI View Article : Google Scholar

30 

Lin Q, Li S, Jiang N, Shao X, Zhang M, Jin H, Zhang Z, Shen J, Zhou Y, Zhou W, et al: PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation. Redox Biol. 26(101254)2019.PubMed/NCBI View Article : Google Scholar

31 

Afonina IS, Zhong Z, Karin M and Beyaert R: Limiting inflammation-the negative regulation of NF-κB and the NLRP3 inflammasome. Nat Immunol. 18:861–869. 2017.PubMed/NCBI View Article : Google Scholar

32 

Meng L, Li L, Lu S, Li K, Su Z, Wang Y, Fan X, Li X and Zhao G: The protective effect of dexmedetomidine on LPS-induced acute lung injury through the HMGB1-mediated TLR4/NF-κB and PI3K/Akt/mTOR pathways. Mol Immunol. 94:7–17. 2018.PubMed/NCBI View Article : Google Scholar

33 

Lin Q, Li S, Jiang N, Jin H, Shao X, Zhu X, Wu J, Zhang M, Zhang Z, Shen J, et al: Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy. Autophagy. 17:2975–2990. 2021.PubMed/NCBI View Article : Google Scholar

34 

Su Y, Wang Y, Liu M and Chen H: Hydrogen sulfide attenuates renal I/R-induced activation of the inflammatory response and apoptosis via regulating Nrf2-mediated NLRP3 signaling pathway inhibition. Mol Med Rep. 24(518)2021.PubMed/NCBI View Article : Google Scholar

35 

Ruan H, Li W, Wang J, Chen G, Xia B, Wang Z and Zhang M: Propofol alleviates ventilator-induced lung injury through regulating the Nrf2/NLRP3 signaling pathway. Exp Mol Pathol. 114(104427)2020.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Qian X and Yang L: ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway. Exp Ther Med 24: 603, 2022.
APA
Qian, X., & Yang, L. (2022). ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway. Experimental and Therapeutic Medicine, 24, 603. https://doi.org/10.3892/etm.2022.11540
MLA
Qian, X., Yang, L."ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway". Experimental and Therapeutic Medicine 24.3 (2022): 603.
Chicago
Qian, X., Yang, L."ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway". Experimental and Therapeutic Medicine 24, no. 3 (2022): 603. https://doi.org/10.3892/etm.2022.11540
Copy and paste a formatted citation
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Spandidos Publications style
Qian X and Yang L: ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway. Exp Ther Med 24: 603, 2022.
APA
Qian, X., & Yang, L. (2022). ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway. Experimental and Therapeutic Medicine, 24, 603. https://doi.org/10.3892/etm.2022.11540
MLA
Qian, X., Yang, L."ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway". Experimental and Therapeutic Medicine 24.3 (2022): 603.
Chicago
Qian, X., Yang, L."ROCK2 knockdown alleviates LPS‑induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF‑κB/NLRP3 signaling pathway". Experimental and Therapeutic Medicine 24, no. 3 (2022): 603. https://doi.org/10.3892/etm.2022.11540
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