Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2022 Volume 24 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2022 Volume 24 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma

  • Authors:
    • Hui Zhao
    • Yongping Wang
    • Weihua Hou
    • Xuanxi Ding
    • Wenji Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 672
    |
    Published online on: September 14, 2022
       https://doi.org/10.3892/etm.2022.11608
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Osteosarcoma (OS) is a common malignant bone cancer and commonly occurs in adolescents and children. Long non‑coding RNAs (lncRNAs) play major roles in cancer cell proliferation and metastasis. The present study aimed to investigate the potential molecular mechanism of lncRNA MALAT1 in OS. The levels of lncRNA MALAT1 and microRNA‑590‑3p were detected by reverse transcription‑quantitative PCR in OS tissues and cells. Cell Counting Kit‑8 and flow cytometry assays were conducted to assess cell proliferation and apoptosis. Cell migration and invasion were examined by Transwell assay. The levels of E‑cadherin, N‑Cadherin, Vimentin and Snail were measured by western blotting. The target of MALAT1 was predicted using online software and confirmed by luciferase reporter, RNA immunoprecipitation and RNA pull‑down assays. The results indicated that MALAT1 was highly expressed in OS tissues and cell lines. MALAT1 knockdown promoted apoptosis and suppressed proliferation, migration, invasion and epithelial‑ mesenchymal transition (EMT) of OS cells. Overexpression of miR‑590‑3p increased cell apoptosis and hampered cell proliferation, migration, invasion and EMT in OS cells. In addition, MALAT1 knockdown upregulated the expression of miR‑590‑3p in OS cells. In conclusion, MALAT1 was demonstrated to suppress cell apoptosis and induce cell proliferation, migration, invasion and EMT by inhibiting miR‑590‑3p in OS, which indicated that MALAT1 has potential value in the diagnosis and treatment of OS.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Luo Z, Xiao L, Li J, Dong B and Wang C: Integrative analysis reveals driver long non-coding RNAs in osteosarcoma. Medicine (Baltimore). 98(e14302)2019.PubMed/NCBI View Article : Google Scholar

2 

Mirabello L, Troisi RJ and Savage SA: International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons. Int J Cancer. 125:229–234. 2009.PubMed/NCBI View Article : Google Scholar

3 

Nie Z and Peng H: Osteosarcoma in patients below 25 years of age: An observational study of incidence, metastasis, treatment and outcomes. Oncol Lett. 16:6502–6514. 2018.PubMed/NCBI View Article : Google Scholar

4 

Luetke A, Meyers PA, Lewis I and Juergens H: Osteosarcoma treatment-where do we stand? A state of the art review. Cancer Treat Rev. 40:523–532. 2014.PubMed/NCBI View Article : Google Scholar

5 

Mercer TR, Dinger ME and Mattick JS: Long non-coding RNAs: Insights into functions. Nat Rev Genet. 10:155–159. 2009.PubMed/NCBI View Article : Google Scholar

6 

Zhang J, Hao X, Yin M, Xu T and Guo F: Long non-coding RNA in osteogenesis: A new world to be explored. Bone Joint Res. 8:73–80. 2019.PubMed/NCBI View Article : Google Scholar

7 

Li JP, Liu LH, Li J, Chen Y, Jiang XW, Ouyang YR, Liu YQ, Zhong H, Li H and Xiao T: Microarray expression profile of long noncoding RNAs in human osteosarcoma. Biochem Biophys Res Commun. 433:200–206. 2013.PubMed/NCBI View Article : Google Scholar

8 

Wang L, Li S, Qi L and Ling L: Long noncoding RNA HCG9 promotes osteosarcoma progression through RAD51 by acting as a ceRNA of miR-34b-3p. Mediators Inflamm: Aug 18, 2021 (Epub ahead of print).

9 

Zhon Y, Feng D, Gu X, Gao A and Liu Y: The role and clinical significance of long noncoding RNA zinc finger E-box-binding homeobox two antisense RNA 1 in promoting osteosarcoma cancer cell proliferation, inhibiting apoptosis and increasing migration by regulating miR-145. Anticancer Drugs. 32:168–177. 2021.PubMed/NCBI View Article : Google Scholar

10 

Ding L, Liu T, Qu Y, Kang Z, Guo L, Zhang H, Jiang J, Qu F, Ge W and Zhang S: lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression. Mol Ther Nucleic Acids. 26:787–797. 2021.PubMed/NCBI View Article : Google Scholar

11 

Chen R, Wang G, Zheng Y, Hua Y and Cai Z: Long non-coding RNAs in osteosarcoma. Oncotarget. 8:20462–20475. 2017.PubMed/NCBI View Article : Google Scholar

12 

Li ZX, Zhu QN, Zhang HB, Hu Y, Wang G and Zhu YS: MALAT1: A potential biomarker in cancer. Cancer Manag Res. 10:6757–6768. 2018.PubMed/NCBI View Article : Google Scholar

13 

Li Z, Yu X and Shen J: Long non-coding RNAs: Emerging players in osteosarcoma. Tumour Biol. 37:2811–2816. 2016.PubMed/NCBI View Article : Google Scholar

14 

Sun Y and Qin B: Long noncoding RNA MALAT1 regulates HDAC4-mediated proliferation and apoptosis via decoying of miR-140-5p in osteosarcoma cells. Cancer Med. 7:4584–4597. 2018.PubMed/NCBI View Article : Google Scholar

15 

Duan G, Zhang C, Xu C, Xu C, Zhang L and Zhang Y: Knockdown of MALAT1 inhibits osteosarcoma progression via regulating the miR34a/cyclin D1 axis. Int J Oncol. 54:17–28. 2019.PubMed/NCBI View Article : Google Scholar

16 

Lynam-Lennon N, Maher SG and Reynolds JV: The roles of microRNA in cancer and apoptosis. Biol Rev Camb Philos Soc. 84:55–71. 2009.PubMed/NCBI View Article : Google Scholar

17 

Di Leva G, Garofalo M and Croce CM: MicroRNAs in cancer. Annu Rev Pathol. 9:287–314. 2014.PubMed/NCBI View Article : Google Scholar

18 

Chen B, Xia Z, Deng YN, Yang Y, Zhang P, Zhu H, Xu N and Liang S: Emerging microRNA biomarkers for colorectal cancer diagnosis and prognosis. Open Biol. 9(180212)2019.PubMed/NCBI View Article : Google Scholar

19 

Pang H, Zheng Y, Zhao Y, Xiu X and Wang J: miR-590-3p suppresses cancer cell migration, invasion and epithelial-mesenchymal transition in glioblastoma multiforme by targeting ZEB1 and ZEB2. Biochem Biophys Res Commun. 468:739–745. 2015.PubMed/NCBI View Article : Google Scholar

20 

Abdolvahabi Z, Nourbakhsh M, Hosseinkhani S, Hesari Z, Alipour M, Jafarzadeh M, Ghorbanhosseini SS, Seiri P, Yousefi Z, Yarahmadi S and Golpour P: MicroRNA-590-3P suppresses cell survival and triggers breast cancer cell apoptosis via targeting sirtuin-1 and deacetylation of p53. J Cell Biochem. 120:9356–9368. 2019.PubMed/NCBI View Article : Google Scholar

21 

Dou Y, Zhu K, Sun Z, Geng X and Fang Q: Screening of disorders associated with osteosarcoma by integrated network analysis. Biosci Rep: May 21, 2019 (Epub ahead of print).

22 

Wang WT, Qi Q, Zhao P, Li CY, Yin XY and Yan RB: miR-590-3p is a novel microRNA which suppresses osteosarcoma progression by targeting SOX9. Biomed Pharmacother. 107:1763–1769. 2018.PubMed/NCBI View Article : Google Scholar

23 

Long J, Bai Y, Yang X, Lin J, Yang X, Wang D, He L, Zheng Y and Zhao H: Construction and comprehensive analysis of a ceRNA network to reveal potential prognostic biomarkers for hepatocellular carcinoma. Cancer Cell Int. 19(90)2019.PubMed/NCBI View Article : Google Scholar

24 

Ren D, Zheng H, Fei S and Zhao JL: MALAT1 induces osteosarcoma progression by targeting miR-206/CDK9 axis. J Cell Physiol. 234:950–957. 2018.PubMed/NCBI View Article : Google Scholar

25 

Sun Z, Zhang T and Chen B: Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) promotes proliferation and metastasis of osteosarcoma cells by targeting c-Met and SOX4 via miR-34a/c-5p and miR-449a/b. Med Sci Monit. 25:1410–1422. 2019.PubMed/NCBI View Article : Google Scholar

26 

Zhang Y, Dai Q, Zeng F and Liu H: MALAT1 Promotes the proliferation and metastasis of osteosarcoma cells by activating the Rac1/JNK pathway via targeting MiR-509. Oncol Res: Apr 27, 2018 (Epub ahead of print).

27 

Chen Y, Huang W, Sun W, Zheng B, Wang C, Luo Z, Wang J and Yan W: LncRNA MALAT1 promotes cancer metastasis in osteosarcoma via activation of the PI3K-Akt signaling pathway. Cell Physiol Biochem. 51:1313–1326. 2018.PubMed/NCBI View Article : Google Scholar

28 

Maximov VV, Akkawi R, Khawaled S, Salah Z, Jaber L, Barhoum A, Or O, Galasso M, Kurek KC, Yavin E and Aqeilan RI: MiR-16-1-3p and miR-16-2-3p possess strong tumor suppressive and antimetastatic properties in osteosarcoma. Int J Cancer. 145:3052–3063. 2019.PubMed/NCBI View Article : Google Scholar

29 

Rohini M, Gokulnath M, Miranda PJ and Selvamurugan N: miR-590-3p inhibits proliferation and promotes apoptosis by targeting activating transcription factor 3 in human breast cancer cells. Biochimie. 154:10–18. 2018.PubMed/NCBI View Article : Google Scholar

30 

Gu L, Lu LS, Zhou DL and Liu ZC: UCA1 promotes cell proliferation and invasion of gastric cancer by targeting CREB1 sponging to miR-590-3p. Cancer Med. 7:1253–1263. 2018.PubMed/NCBI View Article : Google Scholar

31 

Chen L, Wang W, Zhu S, Jin X, Wang J, Zhu J and Zhou Y: MicroRNA-590-3p enhances the radioresistance in glioblastoma cells by targeting LRIG1. Exp Ther Med. 14:1818–1824. 2017.PubMed/NCBI View Article : Google Scholar

32 

Salem M, Shan Y, Bernaudo S and Peng C: miR-590-3p targets cyclin G2 and FOXO3 to promote ovarian cancer cell proliferation, invasion, and spheroid formation. Int J Mol Sci. 20(1810)2019.PubMed/NCBI View Article : Google Scholar

33 

Feng ZY, Xu XH, Cen DZ, Luo CY and Wu SB: miR-590-3p promotes colon cancer cell proliferation via Wnt/beta-catenin signaling pathway by inhibiting WIF1 and DKK1. Eur Rev Med Pharmacol Sci. 21:4844–4852. 2017.PubMed/NCBI

34 

Hu R, Zhu X, Chen C, Xu R, Li Y and Xu W: RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs. Cell Prolif. 51(e12508)2018.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao H, Wang Y, Hou W, Ding X and Wang W: Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma. Exp Ther Med 24: 672, 2022.
APA
Zhao, H., Wang, Y., Hou, W., Ding, X., & Wang, W. (2022). Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma. Experimental and Therapeutic Medicine, 24, 672. https://doi.org/10.3892/etm.2022.11608
MLA
Zhao, H., Wang, Y., Hou, W., Ding, X., Wang, W."Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma". Experimental and Therapeutic Medicine 24.5 (2022): 672.
Chicago
Zhao, H., Wang, Y., Hou, W., Ding, X., Wang, W."Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma". Experimental and Therapeutic Medicine 24, no. 5 (2022): 672. https://doi.org/10.3892/etm.2022.11608
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao H, Wang Y, Hou W, Ding X and Wang W: Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma. Exp Ther Med 24: 672, 2022.
APA
Zhao, H., Wang, Y., Hou, W., Ding, X., & Wang, W. (2022). Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma. Experimental and Therapeutic Medicine, 24, 672. https://doi.org/10.3892/etm.2022.11608
MLA
Zhao, H., Wang, Y., Hou, W., Ding, X., Wang, W."Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma". Experimental and Therapeutic Medicine 24.5 (2022): 672.
Chicago
Zhao, H., Wang, Y., Hou, W., Ding, X., Wang, W."Long non‑coding RNA MALAT1 promotes cell proliferation, migration and invasion by targeting miR‑590‑3p in osteosarcoma". Experimental and Therapeutic Medicine 24, no. 5 (2022): 672. https://doi.org/10.3892/etm.2022.11608
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team