Open Access

KIF2A participates in the progression of hepatocellular carcinoma and angiogenesis by interacting with Notch1

  • Authors:
    • Qi Wu
    • Xuting Ren
    • Yun Chen
    • Ye Jin
    • Xiaobo Zhan
    • Changfeng Liu
    • Bing Zhang
  • View Affiliations

  • Published online on: September 22, 2022     https://doi.org/10.3892/etm.2022.11619
  • Article Number: 683
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Kinesin family member 2A (KIF2A) serves a vital role in the development of hepatocellular carcinoma (HCC); however, the biological effect of KIF2A on the malignant progression of HCC remains unclear. Therefore, the present study was conducted to systematically determine the biological role of KIF2A in HCC and to better understand the molecular mechanism. The differences of KIF2A expression in HHL‑5 normal human hepatocytes and the HCC cell lines Li‑7, Huh7 and MHCC97 were assessed by reverse transcription‑quantitative PCR and western blotting analysis. Moreover, viability, proliferation, migration and invasion of HCC cells were assessed by performing CCK‑8, 5‑ethynyl‑2'‑deoxyuridine staining, wound healing and Transwell assays. Additionally, the tube formation assay was performed to evaluate angiogenesis of HUVECs incubated with the conditioned media of HCC cells in vitro. The interaction between KIF2A and Notch1 was analyzed through co‑immunoprecipitation assay. KIF2A was revealed to be highly expressed in HCC cells. KIF2A knockdown suppressed HCC cell proliferation, migration and invasion, and impaired in vitro angiogenesis. Furthermore, it was revealed that KIF2A interacted with Notch1 and positively regulated Notch1 expression. The suppressive effects of KIF2A knockdown on HCC cell proliferation, migration, invasion and in vitro angiogenesis were partially reversed by Notch1 overexpression. Overall, KIF2A may act as an oncogene in HCC via activation of the Notch1 signaling pathway.

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November-2022
Volume 24 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Wu Q, Ren X, Chen Y, Jin Y, Zhan X, Liu C and Zhang B: KIF2A participates in the progression of hepatocellular carcinoma and angiogenesis by interacting with Notch1. Exp Ther Med 24: 683, 2022
APA
Wu, Q., Ren, X., Chen, Y., Jin, Y., Zhan, X., Liu, C., & Zhang, B. (2022). KIF2A participates in the progression of hepatocellular carcinoma and angiogenesis by interacting with Notch1. Experimental and Therapeutic Medicine, 24, 683. https://doi.org/10.3892/etm.2022.11619
MLA
Wu, Q., Ren, X., Chen, Y., Jin, Y., Zhan, X., Liu, C., Zhang, B."KIF2A participates in the progression of hepatocellular carcinoma and angiogenesis by interacting with Notch1". Experimental and Therapeutic Medicine 24.5 (2022): 683.
Chicago
Wu, Q., Ren, X., Chen, Y., Jin, Y., Zhan, X., Liu, C., Zhang, B."KIF2A participates in the progression of hepatocellular carcinoma and angiogenesis by interacting with Notch1". Experimental and Therapeutic Medicine 24, no. 5 (2022): 683. https://doi.org/10.3892/etm.2022.11619