Thymoquinone‑induced autophagy mitigates doxorubicin‑induced H9c2 cell apoptosis

  • Authors:
    • Dawei Liu
    • Lei Zhao
  • View Affiliations

  • Published online on: September 28, 2022     https://doi.org/10.3892/etm.2022.11630
  • Article Number: 694
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Abstract

Doxorubicin (Dox)‑induced myocardiopathy is a massive obstacle in administering chemotherapeutic drugs in cancer patients. In the present study, the effects of thymoquinone (TQ) on Dox‑induced cardiotoxicity were investigated in vitro. H9c2 cardiomyocytes were pre‑treated with TQ followed by Dox, and cell viability and cell death were subsequently measured. Flow cytometry was conducted to evaluate the apoptosis of H9c2 cells. Autophagy was assessed by immunofluorescence LC3 staining. Western blot analysis was performed to determine autophagy‑, apoptosis‑ and LKB1/AMPK‑related proteins. Dox‑induced H9c2 cell death and apoptosis were decreased after pretreatment with TQ in vitro. The number of autophagosomes and autophagic markers was further increased by TQ in the Dox‑treated H9c2 cells, which mediated LKB1/AMPK activation and the deactivation of mTOR. Suppression of autophagosomes abolished the TQ‑induced antiapoptotic effect. In conclusion, TQ reduces Dox‑induced apoptosis of cardiomyocytes by upregulating autophagy through activation of the LKB1/AMPK pathways. These novel results highlight the therapeutic potential of TQ to prevent Dox cardiotoxicity.
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November-2022
Volume 24 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu D and Zhao L: Thymoquinone‑induced autophagy mitigates doxorubicin‑induced H9c2 cell apoptosis. Exp Ther Med 24: 694, 2022.
APA
Liu, D., & Zhao, L. (2022). Thymoquinone‑induced autophagy mitigates doxorubicin‑induced H9c2 cell apoptosis. Experimental and Therapeutic Medicine, 24, 694. https://doi.org/10.3892/etm.2022.11630
MLA
Liu, D., Zhao, L."Thymoquinone‑induced autophagy mitigates doxorubicin‑induced H9c2 cell apoptosis". Experimental and Therapeutic Medicine 24.5 (2022): 694.
Chicago
Liu, D., Zhao, L."Thymoquinone‑induced autophagy mitigates doxorubicin‑induced H9c2 cell apoptosis". Experimental and Therapeutic Medicine 24, no. 5 (2022): 694. https://doi.org/10.3892/etm.2022.11630