Open Access

VEGF regulates the blood‑brain barrier through MMP‑9 in a rat model of traumatic brain injury

  • Authors:
    • Muyao Wu
    • Yating Gong
    • Lei Jiang
    • Min Zhang
    • Haiping Gu
    • Hui Shen
    • Baoqi Dang
  • View Affiliations

  • Published online on: October 24, 2022     https://doi.org/10.3892/etm.2022.11664
  • Article Number: 728
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Blood‑brain barrier (BBB) damage is closely related to morbidity and mortality in patients with traumatic brain injury (TBI). Inhibition of VEGF effectively protects BBB integrity in clinical ischemic stroke. Protecting BBB integrity, reducing brain edema and alleviating post‑TBI secondary brain injury are key to a favorable patient prognosis. MMP‑9 affects BBB integrity by destroying the tight junction of vascular endothelial cells and inhibiting the transport and enzymatic systems. The present study aimed to examine the possible interplay between VEGF and MMP‑9 in TBI. A TBI model was established in 87 male Sprague‑Dawley rats. Reverse transcription‑quantitative PCR, western blotting, wet‑dry brain edema assessment, TUNEL and Fluoro‑Jade C staining were performed to analyze the brain tissue samples of the rats. The results showed that compared with in the Sham group rats, the mRNA and protein expression levels of VEGF and MMP‑9 were increased at 24 h post‑TBI. After bevacizumab treatment, BBB permeability and nerve cell apoptosis were markedly reduced. In conclusion, the present study revealed a potential role for TBI‑associated VEGF and MMP‑9 upregulation in BBB disruption and nerve damage post‑TBI.
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December-2022
Volume 24 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu M, Gong Y, Jiang L, Zhang M, Gu H, Shen H and Dang B: VEGF regulates the blood‑brain barrier through MMP‑9 in a rat model of traumatic brain injury. Exp Ther Med 24: 728, 2022
APA
Wu, M., Gong, Y., Jiang, L., Zhang, M., Gu, H., Shen, H., & Dang, B. (2022). VEGF regulates the blood‑brain barrier through MMP‑9 in a rat model of traumatic brain injury. Experimental and Therapeutic Medicine, 24, 728. https://doi.org/10.3892/etm.2022.11664
MLA
Wu, M., Gong, Y., Jiang, L., Zhang, M., Gu, H., Shen, H., Dang, B."VEGF regulates the blood‑brain barrier through MMP‑9 in a rat model of traumatic brain injury". Experimental and Therapeutic Medicine 24.6 (2022): 728.
Chicago
Wu, M., Gong, Y., Jiang, L., Zhang, M., Gu, H., Shen, H., Dang, B."VEGF regulates the blood‑brain barrier through MMP‑9 in a rat model of traumatic brain injury". Experimental and Therapeutic Medicine 24, no. 6 (2022): 728. https://doi.org/10.3892/etm.2022.11664