Open Access

Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway

  • Authors:
    • Wenqian Hu
    • Hongjiao Wu
    • Ang Li
    • Xuan Zheng
    • Wenli Zhang
    • Qinqin Tian
    • Xuemei Zhang
  • View Affiliations

  • Published online on: October 26, 2022     https://doi.org/10.3892/etm.2022.11670
  • Article Number: 734
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence has shown that chondroitin sulfate proteoglycan 4 (CSPG4) serve a critical role in tumor progression. However, the roles of chondroitin sulfate proteoglycan 4 pseudogene 12 (CSPG4P12) remain to be elucidated. The present study aimed to investigate the potential effects of CSPG4P12 on the physiological behaviors of non‑small cell lung cancer (NSCLC) and its underlying biological mechanism. The expression levels of CSPG4P12 in NSCLC tissues and adjacent normal tissues were analyzed using the gene expression profiling interactive analysis 2 database and reverse transcription‑quantitative PCR. Cell Counting Kit‑8 and colony formation assays were performed to measure cell proliferation. In addition, Transwell and wound healing assays were performed to assess cell invasion and migration. Cell adhesion was measured by cell‑extracellular matrix adhesion assay. Hoechst 33342 staining assay was performed to detect nucleoli of apoptotic cells, and transmission electron microscopy (TEM) was utilized for apoptosis detection. Immunofluorescence and western blot assays were performed to measure the expression levels of apoptosis‑related proteins. The present results revealed that the expression levels of CSPG4P12 in NSCLC tissues were significantly lower compared with those in adjacent normal tissues. Overexpression of CSPG4P12 inhibited cell proliferation, invasion, migration and adhesion whilst promoting apoptosis. There were missing mitochondrial cristae and mitochondrial vacuoles in the CSPG4P12‑overexpressed cells when observed under TEM. Overexpression of CSPG4P12 also increased the expression of Bax and p53, whereas it inhibited the expression of Bcl2. In conclusion, CSPG4P12 could inhibit NSCLC development and tumorigenesis by activating the p53/Bcl2/Bax mitochondrial apoptotic pathway.
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December-2022
Volume 24 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hu W, Wu H, Li A, Zheng X, Zhang W, Tian Q and Zhang X: Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway. Exp Ther Med 24: 734, 2022
APA
Hu, W., Wu, H., Li, A., Zheng, X., Zhang, W., Tian, Q., & Zhang, X. (2022). Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway. Experimental and Therapeutic Medicine, 24, 734. https://doi.org/10.3892/etm.2022.11670
MLA
Hu, W., Wu, H., Li, A., Zheng, X., Zhang, W., Tian, Q., Zhang, X."Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway". Experimental and Therapeutic Medicine 24.6 (2022): 734.
Chicago
Hu, W., Wu, H., Li, A., Zheng, X., Zhang, W., Tian, Q., Zhang, X."Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway". Experimental and Therapeutic Medicine 24, no. 6 (2022): 734. https://doi.org/10.3892/etm.2022.11670