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Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway

  • Authors:
    • Zhi-Xing Fan
    • Chao-Jun Yang
    • Ya-Hui Li
    • Jian Yang
    • Cong-Xin Huang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei 443000, P.R. China, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 35
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    Published online on: November 29, 2022
       https://doi.org/10.3892/etm.2022.11734
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Abstract

Ginsenoside Rh2 (GRh2) is a monomer isolated from red ginseng that has extensive pharmacological effects. However, whether GRh2 has a protective effect on ischaemia/reperfusion (I/R) in the myocardium has yet to be elucidated. The present study aimed to identify the anti‑inflammatory and antioxidant effects of GRh2 on I/R in the myocardium and its underlying mechanism. A rat model of myocardial I/R injury was constructed by ligating the left anterior descending coronary artery, which was subsequently treated with GRh2. A total of 40 male Sprague‑Dawley rats were divided into the following four groups: The sham group, the I/R group, the I/R+GRh2 (10 mg/kg) group and the I/R+GRh2 (20 mg/kg) group. Neonatal rat cardiomyocytes were also used to evaluate the protective effect of GRh2 on hypoxia/reoxygenation (H/R)‑induced myocardial injury in vitro. The GRh2 pre‑treatment reduced the I/R‑ or H/R‑induced release of myocardial enzymes and the production of IL‑1β, IL‑18 and TNF‑α. GRh2 reduced the area of myocardial infarction and the histological changes in the myocardium and improved cardiac functions. In addition, GRh2 reduced the expression levels of NOD‑like receptor family pyrin domain‑containing 3 (NLRP3), apoptosis‑associated speck‑like protein, caspase‑1, malondialdehyde and reactive oxygen species and increased the expression levels of nuclear factor E2‑related factor 2 (Nrf2), heme oxygenase‑1 (HO‑1), glutathione peroxidase and superoxide dismutase. In conclusion, the present study confirmed that GRh2 could reduce oxidative stress and inflammation in cardiomyocytes after reperfusion, and its mechanism of action may be related to its regulation of the Nrf2/HO‑1/NLRP3 signalling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Fan Z, Yang C, Li Y, Yang J and Huang C: Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway. Exp Ther Med 25: 35, 2023.
APA
Fan, Z., Yang, C., Li, Y., Yang, J., & Huang, C. (2023). Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway. Experimental and Therapeutic Medicine, 25, 35. https://doi.org/10.3892/etm.2022.11734
MLA
Fan, Z., Yang, C., Li, Y., Yang, J., Huang, C."Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway". Experimental and Therapeutic Medicine 25.1 (2023): 35.
Chicago
Fan, Z., Yang, C., Li, Y., Yang, J., Huang, C."Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway". Experimental and Therapeutic Medicine 25, no. 1 (2023): 35. https://doi.org/10.3892/etm.2022.11734
Copy and paste a formatted citation
x
Spandidos Publications style
Fan Z, Yang C, Li Y, Yang J and Huang C: Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway. Exp Ther Med 25: 35, 2023.
APA
Fan, Z., Yang, C., Li, Y., Yang, J., & Huang, C. (2023). Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway. Experimental and Therapeutic Medicine, 25, 35. https://doi.org/10.3892/etm.2022.11734
MLA
Fan, Z., Yang, C., Li, Y., Yang, J., Huang, C."Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway". Experimental and Therapeutic Medicine 25.1 (2023): 35.
Chicago
Fan, Z., Yang, C., Li, Y., Yang, J., Huang, C."Ginsenoside Rh2 attenuates myocardial ischaemia‑reperfusion injury by regulating the Nrf2/HO‑1/NLRP3 signalling pathway". Experimental and Therapeutic Medicine 25, no. 1 (2023): 35. https://doi.org/10.3892/etm.2022.11734
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