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Identification of ferroptosis‑associated genes in chronic kidney disease

  • Authors:
    • Lishi Shao
    • Qixiang Fang
    • Chaofei Ba
    • Yanqing Zhang
    • Chen Shi
    • Ya Zhang
    • Jiaping Wang
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Kunming Medical University and The Second Affiliated Hospital, Kunming, Yunnan 650500, P.R. China, Department of Urology, The First Affiliated Hospital of The Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710049, P.R. China, Department of Radiology, Kunming Children's Hospital, Kunming, Yunnan 650034, P.R. China, Department of Radiology, Kunming Medical University and The Third Affiliated Hospital, Kunming, Yunnan 650500, P.R. China
    Copyright: © Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 60
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    Published online on: December 8, 2022
       https://doi.org/10.3892/etm.2022.11759
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Abstract

Ferroptosis serves a pivotal role in developing chronic kidney disease (CKD). The present study aimed to detect and confirm the relevance of potential ferroptosis‑related genes in CKD using bioinformatics and experimentation strategies. The original GSE15072 mRNA expression dataset was retrieved from the Gene Expression Omnibus database. Subsequently, the potential differentially expressed genes associated with ferroptosis of CKD were screened using R software. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analyses, correlation analysis and protein‑protein interactions (PPI) were performed for differentially expressed ferroptosis‑associated genes (DFGs). Lastly, the expression levels of the top nine DFGs were measured in the kidney tissue of Adriamycin‑induced CKD rats and healthy controls via reverse transcription‑quantitative (RT‑q)PCR analysis. Overall, 49 DFGs among 21 patients with CKD and nine healthy controls were identified. GO and KEGG enrichment analyses demonstrated that these DFGs were primarily involved in ‘ferroptosis’ and ‘mitophagy’. PPI findings indicated that these ferroptosis‑associated genes interacted with one another. RT‑qPCR of CKD tissue from the rat model revealed that STAT3, MAPK14, heat shock protein (HSP)A5, MTOR and solute carrier family 2 member 1 (SLC2A1) mRNA levels in CKD were upregulated. Overall, 49 potential ferroptosis‑associated genes of CKD were identified via bioinformatics analyses. STAT3, MAPK14, HSPA5, MTOR and SLC2A1 may influence CKD onset by regulating ferroptosis. The present results add to the existing body of knowledge about CKD and may be useful in the treatment of CKD.
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Copy and paste a formatted citation
Spandidos Publications style
Shao L, Fang Q, Ba C, Zhang Y, Shi C, Zhang Y and Wang J: Identification of ferroptosis‑associated genes in chronic kidney disease. Exp Ther Med 25: 60, 2023.
APA
Shao, L., Fang, Q., Ba, C., Zhang, Y., Shi, C., Zhang, Y., & Wang, J. (2023). Identification of ferroptosis‑associated genes in chronic kidney disease. Experimental and Therapeutic Medicine, 25, 60. https://doi.org/10.3892/etm.2022.11759
MLA
Shao, L., Fang, Q., Ba, C., Zhang, Y., Shi, C., Zhang, Y., Wang, J."Identification of ferroptosis‑associated genes in chronic kidney disease". Experimental and Therapeutic Medicine 25.1 (2023): 60.
Chicago
Shao, L., Fang, Q., Ba, C., Zhang, Y., Shi, C., Zhang, Y., Wang, J."Identification of ferroptosis‑associated genes in chronic kidney disease". Experimental and Therapeutic Medicine 25, no. 1 (2023): 60. https://doi.org/10.3892/etm.2022.11759
Copy and paste a formatted citation
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Spandidos Publications style
Shao L, Fang Q, Ba C, Zhang Y, Shi C, Zhang Y and Wang J: Identification of ferroptosis‑associated genes in chronic kidney disease. Exp Ther Med 25: 60, 2023.
APA
Shao, L., Fang, Q., Ba, C., Zhang, Y., Shi, C., Zhang, Y., & Wang, J. (2023). Identification of ferroptosis‑associated genes in chronic kidney disease. Experimental and Therapeutic Medicine, 25, 60. https://doi.org/10.3892/etm.2022.11759
MLA
Shao, L., Fang, Q., Ba, C., Zhang, Y., Shi, C., Zhang, Y., Wang, J."Identification of ferroptosis‑associated genes in chronic kidney disease". Experimental and Therapeutic Medicine 25.1 (2023): 60.
Chicago
Shao, L., Fang, Q., Ba, C., Zhang, Y., Shi, C., Zhang, Y., Wang, J."Identification of ferroptosis‑associated genes in chronic kidney disease". Experimental and Therapeutic Medicine 25, no. 1 (2023): 60. https://doi.org/10.3892/etm.2022.11759
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