Open Access

Clinical utility of MR spectroscopy for gynecological pelvic abscesses using next‑generation sequencing technology for the detection of causative bacteria

  • Authors:
    • Yuya Nogami
    • Kouji Banno
    • Yusuke Kobayashi
    • Eiichiro Tominaga
    • Shigeo Okuda
    • Daisuke Aoki
  • View Affiliations

  • Published online on: January 12, 2023     https://doi.org/10.3892/etm.2023.11796
  • Article Number: 96
  • Copyright: © Nogami et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Due to the invasiveness of sample collection, treatment for an abscess in the pelvis, such as a gynecological abscess, is often started without a culture test. A test that could predict the appropriate antibiotic and clinical course without invasiveness prior to treatment initiation would be useful. Magnetic resonance spectroscopy (MRS) can be used to detect metabolites in an abscess and has the potential for evaluation of gynecological abscesses. The present study investigated the use of MRS for the evaluation of gynecological abscesses, using next‑generation sequencing (NGS) for detection of true pathogenic bacteria. A total of 16 patients with a gynecological abscess who were treated at Keio University Hospital (Tokyo, Japan) from July 2015 to September 2016 and underwent MRS were recruited to the present study. If available, samples from drainage or surgery were used for detection of true pathogenic bacteria based on analyses of bacterial flora using NGS of 16S ribosomal DNA. MRS signals, NGS results and clinical course were then compared. All patients gave written informed consent after receiving an oral explanation of the study and the study was approved by the institutional research ethics committee. Of the 16 patients, six had MRS signals with a specific peak at 1.33 ppm, which suggested the presence of lipid or lactic acid. However, there was no significant association between metabolism, MRS signals, pathogenesis and clinical course. Only in cases of infectious lymphocele were there cases with a lactic acid peak that seemed to improve without drainage. In conclusion, the present study was not able to show marked usefulness of MRS for the identification of pathogenic bacteria and prediction of the clinical course; however, MRS may be useful for predicting the need for drainage in patients with infectious lymphocele. This study was registered as a clinical trial in the UMIN Clinical Trials Registry (registration no. UMIN000016705) on March 11, 2015.

Related Articles

Journal Cover

February-2023
Volume 25 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Nogami Y, Banno K, Kobayashi Y, Tominaga E, Okuda S and Aoki D: Clinical utility of MR spectroscopy for gynecological pelvic abscesses using next‑generation sequencing technology for the detection of causative bacteria. Exp Ther Med 25: 96, 2023
APA
Nogami, Y., Banno, K., Kobayashi, Y., Tominaga, E., Okuda, S., & Aoki, D. (2023). Clinical utility of MR spectroscopy for gynecological pelvic abscesses using next‑generation sequencing technology for the detection of causative bacteria. Experimental and Therapeutic Medicine, 25, 96. https://doi.org/10.3892/etm.2023.11796
MLA
Nogami, Y., Banno, K., Kobayashi, Y., Tominaga, E., Okuda, S., Aoki, D."Clinical utility of MR spectroscopy for gynecological pelvic abscesses using next‑generation sequencing technology for the detection of causative bacteria". Experimental and Therapeutic Medicine 25.2 (2023): 96.
Chicago
Nogami, Y., Banno, K., Kobayashi, Y., Tominaga, E., Okuda, S., Aoki, D."Clinical utility of MR spectroscopy for gynecological pelvic abscesses using next‑generation sequencing technology for the detection of causative bacteria". Experimental and Therapeutic Medicine 25, no. 2 (2023): 96. https://doi.org/10.3892/etm.2023.11796