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Icariin protects cerebral neural cells from ischemia‑reperfusion injury in an in vitro model by lowering ROS production and intracellular calcium concentration

  • Authors:
    • Ke Ning
    • Rong Gao
  • View Affiliations

  • Published online on: February 16, 2023     https://doi.org/10.3892/etm.2023.11849
  • Article Number: 151
  • Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemia is one of the major causes of stroke. The present study investigated the protection of cultured neural cells by icariin (ICA) against ischemia‑reperfusion (I/R) injury and possible mechanisms underlying the protection. Neural cells were isolated from neonatal rats and cultured in vitro. The cells were subjected to oxygen‑glucose deprivation and reoxygenation (OGD‑R) as an I/R mimic to generate I/R injury, and were post‑OGD‑R treated with ICA. Following the treatments, cell viability, apoptosis, reactive oxygen species (ROS), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and Ca2+ concentration were assessed using Cell Counting Kit‑8 assay, flow cytometry, CyQUANT™ LDH Cytotoxicity Assay, H2DCFDA and SOD colorimetric activity kit. After OGD‑R, considerable I/R injury was observed in the neural cells, as indicated by reduced cell viability, increased apoptosis and increased production of ROS and LDH (P<0.05). Cellular Ca2+ concentration was also increased, while SOD activity remained unchanged. Post‑OGD‑R ICA treatments increased cell viability up to 87.1% (P<0.05) and reduced apoptosis as low as 6.6% (P<0.05) in a concentration‑dependent manner. The treatments also resulted in fewer ROS (P<0.05), lower extracellular LDH content (440.5 vs. 230.3 U/l; P<0.05) and reduced Ca2+ increase (P<0.05). These data suggest that ICA protects the neural cells from I/R injury in an in vitro model through antioxidation activity and maintaining cellular Ca2+ homeostasis. This function may be explored as a potential therapeutic strategy for ischemia‑related diseases after further in vivo studies.
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April-2023
Volume 25 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ning K and Ning K: Icariin protects cerebral neural cells from ischemia‑reperfusion injury in an <em>in vitro</em> model by lowering ROS production and intracellular calcium concentration. Exp Ther Med 25: 151, 2023
APA
Ning, K., & Ning, K. (2023). Icariin protects cerebral neural cells from ischemia‑reperfusion injury in an <em>in vitro</em> model by lowering ROS production and intracellular calcium concentration. Experimental and Therapeutic Medicine, 25, 151. https://doi.org/10.3892/etm.2023.11849
MLA
Ning, K., Gao, R."Icariin protects cerebral neural cells from ischemia‑reperfusion injury in an <em>in vitro</em> model by lowering ROS production and intracellular calcium concentration". Experimental and Therapeutic Medicine 25.4 (2023): 151.
Chicago
Ning, K., Gao, R."Icariin protects cerebral neural cells from ischemia‑reperfusion injury in an <em>in vitro</em> model by lowering ROS production and intracellular calcium concentration". Experimental and Therapeutic Medicine 25, no. 4 (2023): 151. https://doi.org/10.3892/etm.2023.11849