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Article Open Access

A novel type of serum‑free medium for culturing human airway epithelium cells

  • Authors:
    • Haotian Dai
    • Qi Zheng
    • Shan Shan
    • Yuqiong Lei
    • Jingyuan Zhang
    • Cheng Zhong
    • Yongle Xu
    • Zeyu Liu
    • Tao Ren
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, P.R. China
    Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 239
    |
    Published online on: April 5, 2023
       https://doi.org/10.3892/etm.2023.11938
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Abstract

The present study aimed to evaluate the ability of a novel serum‑free medium (SFM) to culture human airway epithelium cells (hAECs). hAECs were cultured in the novel SFM as the experimental group in the PneumaCult‑Ex medium and Dulbecco's modified eagle medium (DMEM) and fetal bovine serum (FBS) as the control groups. Cell morphology, proliferative capacity, differentiation capacity and expression levels of basal cell markers were assessed accordingly in both culture systems. Optical microscope photos of hAECs were collected for cell morphology assessment. Cell Counting Kit‑8 assay was conducted to assess the proliferation ability, and an air‑liquid interface (ALI) assay was conducted to assess the differentiation capacity. Markers for proliferating basal and differentiated cells were relatively identified by immunohistochemical and immunofluorescent analysis. The results show that whether grown in the novel SFM or Ex medium, hAECs exhibited similar morphology at every passage, whereas cells could hardly form colonies in the DMEM + FBS group. Cells typically exhibited cobblestone shape, while a proportion of them in the novel SFM at late passage exhibited a larger shape. White vesicles appeared in the cytoplasm of some control cells at the later stage of culture. Basal cell markers (P63+KRT5+KI67+CC10‑) for proliferating ability were found in the hAECs cultured by the novel SFM and Ex medium. hAECs at passage 3 cultured in the novel SFM and Ex medium both had the capacity to differentiate into ciliated cells (acetylated tubulin+), goblet cells (MUC5AC+) and club cells (CC10+) in the ALI culture assay. In conclusion, the novel SFM was capable of culturing hAECs. The hAECs cultured by the novel SFM could proliferate and differentiate in vitro. The novel SFM does not change the morphological characteristics or biomarkers of hAECs. The novel SFM has the potential for the amplification of hAECs for scientific research and clinical application.
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Copy and paste a formatted citation
Spandidos Publications style
Dai H, Zheng Q, Shan S, Lei Y, Zhang J, Zhong C, Xu Y, Liu Z and Ren T: A novel type of serum‑free medium for culturing human airway epithelium cells. Exp Ther Med 25: 239, 2023.
APA
Dai, H., Zheng, Q., Shan, S., Lei, Y., Zhang, J., Zhong, C. ... Ren, T. (2023). A novel type of serum‑free medium for culturing human airway epithelium cells. Experimental and Therapeutic Medicine, 25, 239. https://doi.org/10.3892/etm.2023.11938
MLA
Dai, H., Zheng, Q., Shan, S., Lei, Y., Zhang, J., Zhong, C., Xu, Y., Liu, Z., Ren, T."A novel type of serum‑free medium for culturing human airway epithelium cells". Experimental and Therapeutic Medicine 25.5 (2023): 239.
Chicago
Dai, H., Zheng, Q., Shan, S., Lei, Y., Zhang, J., Zhong, C., Xu, Y., Liu, Z., Ren, T."A novel type of serum‑free medium for culturing human airway epithelium cells". Experimental and Therapeutic Medicine 25, no. 5 (2023): 239. https://doi.org/10.3892/etm.2023.11938
Copy and paste a formatted citation
x
Spandidos Publications style
Dai H, Zheng Q, Shan S, Lei Y, Zhang J, Zhong C, Xu Y, Liu Z and Ren T: A novel type of serum‑free medium for culturing human airway epithelium cells. Exp Ther Med 25: 239, 2023.
APA
Dai, H., Zheng, Q., Shan, S., Lei, Y., Zhang, J., Zhong, C. ... Ren, T. (2023). A novel type of serum‑free medium for culturing human airway epithelium cells. Experimental and Therapeutic Medicine, 25, 239. https://doi.org/10.3892/etm.2023.11938
MLA
Dai, H., Zheng, Q., Shan, S., Lei, Y., Zhang, J., Zhong, C., Xu, Y., Liu, Z., Ren, T."A novel type of serum‑free medium for culturing human airway epithelium cells". Experimental and Therapeutic Medicine 25.5 (2023): 239.
Chicago
Dai, H., Zheng, Q., Shan, S., Lei, Y., Zhang, J., Zhong, C., Xu, Y., Liu, Z., Ren, T."A novel type of serum‑free medium for culturing human airway epithelium cells". Experimental and Therapeutic Medicine 25, no. 5 (2023): 239. https://doi.org/10.3892/etm.2023.11938
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