Open Access

Alleviating effect of lycorine on CFA‑induced arthritic pain via inhibition of spinal inflammation and oxidative stress

  • Authors:
    • Yin-Di Hu
    • Yuan-Fen Yue
    • Tao Chen
    • Zhao-Di Wang
    • Jie-Qing Ding
    • Min Xie
    • Dai Li
    • Hai-Li Zhu
    • Meng-Lin Cheng
  • View Affiliations

  • Published online on: April 11, 2023     https://doi.org/10.3892/etm.2023.11940
  • Article Number: 241
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic pain is the primary symptom of osteoarthritis affecting a patient's quality of life. Neuroinflammation and oxidative stress in the spinal cord contribute to arthritic pain and represent ideal targets for pain management. In the present study, a model of arthritis was established by intra‑articular injection of complete Freund's adjuvant (CFA) into the left knee joint in mice. After CFA inducement, knee width and pain hypersensitivity in the mice were increased, motor disability was impaired, spinal inflammatory reaction was induced, spinal astrocytes were activated, antioxidant responses were decreased, and glycogen synthase kinase 3β (GSK‑3β) activity was inhibited. To explore the potential therapeutic options for arthritic pain, lycorine was intraperitoneally injected for 3 days in the CFA mice. Lycorine treatment significantly reduced mechanical pain sensitivity, suppressed spontaneous pain, and recovered motor coordination in the CFA‑induced mice. Additionally, in the spinal cord, lycorine treatment decreased the inflammatory score, reduced NOD‑like receptor protein 3 inflammasome (NLRP3) activity and IL‑1β expression, suppressed astrocytic activation, downregulated NF‑κB levels, increased nuclear factor erythroid 2‑related factor 2 expression and superoxide dismutase activity. Furthermore, lycorine was shown to bind to GSK‑3β through three electrovalent bonds, to inhibit GSK‑3β activity. In summary, lycorine treatment inhibited GSK‑3β activity, suppressed NLRP3 inflammasome activation, increased the antioxidant response, reduced spinal inflammation, and relieved arthritic pain.
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June-2023
Volume 25 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hu Y, Yue Y, Chen T, Wang Z, Ding J, Xie M, Li D, Zhu H and Cheng M: Alleviating effect of lycorine on CFA‑induced arthritic pain via inhibition of spinal inflammation and oxidative stress. Exp Ther Med 25: 241, 2023
APA
Hu, Y., Yue, Y., Chen, T., Wang, Z., Ding, J., Xie, M. ... Cheng, M. (2023). Alleviating effect of lycorine on CFA‑induced arthritic pain via inhibition of spinal inflammation and oxidative stress. Experimental and Therapeutic Medicine, 25, 241. https://doi.org/10.3892/etm.2023.11940
MLA
Hu, Y., Yue, Y., Chen, T., Wang, Z., Ding, J., Xie, M., Li, D., Zhu, H., Cheng, M."Alleviating effect of lycorine on CFA‑induced arthritic pain via inhibition of spinal inflammation and oxidative stress". Experimental and Therapeutic Medicine 25.6 (2023): 241.
Chicago
Hu, Y., Yue, Y., Chen, T., Wang, Z., Ding, J., Xie, M., Li, D., Zhu, H., Cheng, M."Alleviating effect of lycorine on CFA‑induced arthritic pain via inhibition of spinal inflammation and oxidative stress". Experimental and Therapeutic Medicine 25, no. 6 (2023): 241. https://doi.org/10.3892/etm.2023.11940