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Article

Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells in vitro

  • Authors:
    • Sai He
    • Yanni Hou
    • Leina Hou
    • Nan Chen
    • Xiaomin Yang
    • Huxia Wang
    • Pihua Han
    • Yongguo Fan
    • Jing Zhao
    • Jingyuan Zhang
    • Jie Geng
  • View Affiliations / Copyright

    Affiliations: Department of Breast Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi 710061, P.R. China, Department of Anesthesiology, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi 710061, P.R. China, Department of Cardiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Article Number: 245
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    Published online on: April 11, 2023
       https://doi.org/10.3892/etm.2023.11944
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Abstract

Human epidermal growth factor receptor 2‑positive (HER2+) breast cancer, which accounts for 15‑20% of all breast cancer, is associated with tumor recurrence and poor prognosis. RAS association domain family protein 1 subtype A (RASSF1A) is a tumor suppressor that is silenced in a variety of human cancers. The present study aimed to investigate the role of RASSF1A in HER2+ breast cancer and the therapeutic potential of RASSF1A‑based targeted gene therapy for this malignancy. RASSF1A expression in human HER2+ breast cancer tissues and cell lines was evaluated by reverse transcription PCR and western blot analysis. The associations between tumorous RASSF1A level and tumor grade, TNM stage, tumor size, lymph node metastasis and five‑year survival were examined. HER2+ and HER2‑negative (HER2‑) breast cancer cells were transfected with a lentiviral vector (LV‑5HH‑RASSF1A) that could express RASSF1A under the control of five copies of the hypoxia‑responsive element (5HRE) and one copy of the HER2 promoter (HER2p). Cell proliferation was evaluated by the MTT and colony formation assays. It was found that tumorous RASSF1A level was negatively associated with tumor grade (P=0.014), TNM stage (P=0.0056), tumor size (P=0.014) and lymph node metastasis (P=0.029) and positively associated with five‑year survival (P=0.038) in HER2+ breast cancer patients. Lentiviral transfection of HER2+ breast cancer cells resulted in increased RASSF1A expression and decreased cell proliferation, especially under hypoxic conditions. However, lentiviral transfection of HER2‑breast cancer cells did not affect RASSF1A expression. In conclusion, these findings verified the clinical significance of RASSF1A as a tumor suppressor in HER2+ breast cancer and supported LV‑5HH‑RASSF1A as a potential targeted gene therapy for this malignancy.
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1 

Waks AG and Winer EP: Breast cancer treatment: A review. JAMA. 321:288–300. 2019.PubMed/NCBI View Article : Google Scholar

2 

Iancu G, Serban D, Badiu CD, Tanasescu C, Tudosie MS, Tudor C, Costea DO, Zgura A, Iancu R and Vasile D: Tyrosine kinase inhibitors in breast cancer (review). Exp Ther Med. 23(114)2022.PubMed/NCBI View Article : Google Scholar

3 

Ding S, Sun X, Lu S, Wang Z, Chen X and Shen K: Association of molecular subtype concordance and survival outcome in synchronous and metachronous bilateral breast cancer. Breast. 57:71–79. 2021.PubMed/NCBI View Article : Google Scholar

4 

Howlader N, Altekruse SF, Li CI, Chen VW, Clarke CA, Ries LA and Cronin KA: US incidence of breast cancer subtypes defined by joint hormone receptor and HER2 status. J Natl Cancer Inst. 106(dju055)2014.PubMed/NCBI View Article : Google Scholar

5 

Slamon DJ, Clark GM, Wong SG, Levin WJ, Ullrich A and McGuire WL: Human breast cancer: Correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science. 235:177–182. 1987.PubMed/NCBI View Article : Google Scholar

6 

Howlader N, Cronin KA, Kurian AW and Andridge R: Differences in breast cancer survival by molecular subtypes in the United States. Cancer Epidemiol Biomarkers Prev. 27:619–626. 2018.PubMed/NCBI View Article : Google Scholar

7 

Pallerla S, Abdul AURM, Comeau J and Jois S: Cancer vaccines, treatment of the future: With emphasis on HER2-positive breast cancer. Int J Mol Sci. 22(779)2021.PubMed/NCBI View Article : Google Scholar

8 

Takada M and Toi M: Neoadjuvant treatment for HER2-positive breast cancer. Chin Clin Oncol. 9(32)2020.PubMed/NCBI View Article : Google Scholar

9 

Choong GM, Cullen GD and O'Sullivan CC: Evolving standards of care and new challenges in the management of HER2-positive breast cancer. CA Cancer J Clin. 70:355–374. 2020.PubMed/NCBI View Article : Google Scholar

10 

Hesson LB, Cooper WN and Latif F: The role of RASSF1A methylation in cancer. Dis Markers. 23:73–87. 2007.PubMed/NCBI View Article : Google Scholar

11 

Li M, Wang C, Yu B, Zhang X, Shi F and Liu X: Diagnostic value of RASSF1A methylation for breast cancer: A meta-analysis. Biosci Rep. 39(BSR20190923)2019.PubMed/NCBI View Article : Google Scholar

12 

Thaler S, Schmidt M, Schad A and Sleeman JP: RASSF1A inhibits estrogen receptor alpha expression and estrogen-independent signalling: Implications for breast cancer development. Oncogene. 31:4912–4922. 2012.PubMed/NCBI View Article : Google Scholar

13 

Roßwag S, Sleeman JP and Thaler S: RASSF1A-mediated suppression of estrogen receptor alpha (ERα)-driven breast cancer cell growth depends on the Hippo-kinases LATS1 and 2. Cells. 10(2868)2021.PubMed/NCBI View Article : Google Scholar

14 

Sun W, Shi Q, Zhang H, Yang K, Ke Y, Wang Y and Qiao L: Advances in the techniques and methodologies of cancer gene therapy. Discov Med. 27:45–55. 2019.PubMed/NCBI

15 

Hong R and Xu B: Breast cancer: An up-to-date review and future perspectives. Cancer Commun (Lond). 42:913–936. 2022.PubMed/NCBI View Article : Google Scholar

16 

Sun W, Liu XY, Ma LL and Lu ZL: Tumor targeting gene vector for visual tracking of Bcl-2 siRNA transfection and anti-tumor therapy. ACS Appl Mater Interfaces. 12:10193–10201. 2020.PubMed/NCBI View Article : Google Scholar

17 

Montaño-Samaniego M, Bravo-Estupiñan DM, Méndez-Guerrero O, Alarcón-Hernández E and Ibáñez-Hernández M: Strategies for targeting gene therapy in cancer cells with tumor-specific promoters. Front Oncol. 10(605380)2020.PubMed/NCBI View Article : Google Scholar

18 

Altwaijry N, Somani S and Dufès C: Targeted nonviral gene therapy in prostate cancer. Int J Nanomedicine. 13:5753–5767. 2018.PubMed/NCBI View Article : Google Scholar

19 

Chen C, Yue D, Lei L, Wang H, Lu J, Zhou Y, Liu S, Ding T, Guo M and Xu L: Promoter-operating targeted expression of gene therapy in cancer: Current stage and prospect. Mol Ther Nucleic Acids. 11:508–514. 2018.PubMed/NCBI View Article : Google Scholar

20 

Hurst HC: Update on HER-2 as a target for cancer therapy: The ERBB2 promoter and its exploitation for cancer treatment. Breast Cancer Res. 3:395–398. 2001.PubMed/NCBI View Article : Google Scholar

21 

Cui X, Chen H, Zhang Q, Xu M, Yuan G and Zhou J: Exploration of the structure and recognition of a G-quadruplex in the her2 proto-oncogene promoter and its transcriptional regulation. Sci Rep. 9(3966)2019.PubMed/NCBI View Article : Google Scholar

22 

Lundgren K, Holm C and Landberg G: Hypoxia and breast cancer: Prognostic and therapeutic implications. Cell Mol Life Sci. 64:3233–3247. 2007.PubMed/NCBI View Article : Google Scholar

23 

Wu D, Potluri N, Lu J, Kim Y and Rastinejad F: Structural integration in hypoxia-inducible factors. Nature. 524:303–308. 2015.PubMed/NCBI View Article : Google Scholar

24 

Lakhani SR, Ellis IO, Schnitt SJ, Tan PH and van de Vijver MJ (eds): WHO classification of tumors of the breast. 4th edtion. Lyon, IARC Press, 2012.

25 

You D, Wang D, Liu P, Chu Y, Zhang X, Ding X, Li X, Mao T, Jing X, Tian Z and Pan Y: MicroRNA-498 inhibits the proliferation, migration and invasion of gastric cancer through targeting BMI-1 and suppressing AKT pathway. Human Cell. 33:366–376. 2020.PubMed/NCBI View Article : Google Scholar

26 

Luo M, Hou L, Li J, Shao S, Huang S, Meng D, Liu L, Feng L, Xia P, Qin T and Zhao X: VEGF/NRP-1axis promotes progression of breast cancer via enhancement of epithelial-mesenchymal transition and activation of NF-κB and β-catenin. Cancer Lett. 373:1–11. 2016.PubMed/NCBI View Article : Google Scholar

27 

Chen N, He S, Geng J, Song ZJ, Han PH, Qin J, Zhao Z, Song YC, Wang HX and Dang CX: Overexpression of Contactin 1 promotes growth, migration and invasion in Hs578T breast cancer cells. BMC Cell Biol. 19(5)2018.PubMed/NCBI View Article : Google Scholar

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

29 

Zhou PH, Zheng JB, Wei GB, Wang XL, Wang W, Chen NZ, Yu JH, Yao JF, Wang H, Lu SY and Sun XJ: Lentivirus-mediated RASSF1A expression suppresses aggressive phenotypes of gastric cancer cells in vitro and in vivo. Gene Ther. 22:793–801. 2015.PubMed/NCBI View Article : Google Scholar

30 

He S, Sun XJ, Zheng JB, Qi J, Chen NZ, Wang W, Wei GB, Liu D, Yu JH, Lu SY and Wang H: Recombinant lentivirus with enhanced expression of caudal-related homeobox protein 2 inhibits human colorectal cancer cell proliferation in vitro. Mol Med Rep. 12:1838–1844. 2015.PubMed/NCBI View Article : Google Scholar

31 

Kreutzfeldt J, Rozeboom B, Dey N and De P: The trastuzumab era: Current and upcoming targeted HER2+ breast cancer therapies. Am J Cancer Res. 10:1045–1067. 2020.PubMed/NCBI

32 

Murthy RK, Loi S, Okines A, Paplomata E, Hamilton E, Hurvitz SA, Lin NU, Borges V, Abramson V, Anders C, et al: Tucatinib, trastuzumab, and capecitabine for HER2-positive metastatic breast cancer. N Engl J Med. 382:597–609. 2020.PubMed/NCBI View Article : Google Scholar

33 

Tesch ME and Gelmon KA: Targeting HER2 in breast cancer: Latest developments on treatment sequencing and the introduction of biosimilars. Drugs. 80:1811–1830. 2020.PubMed/NCBI View Article : Google Scholar

34 

Arabi F, Mansouri V and Ahmadbeigi N: Gene therapy clinical trials, where do we go? An overview. Biomed Pharmacother. 153(113324)2022.PubMed/NCBI View Article : Google Scholar

35 

Dastjerd NT, Valibeik A, Rahimi Monfared S, Goodarzi G, Moradi Sarabi M, Hajabdollahi F, Maniati M, Amri J and Samavarchi Tehrani S: Gene therapy: A promising approach for breast cancer treatment. Cell Biochem Funct. 40:28–48. 2022.PubMed/NCBI View Article : Google Scholar

36 

Xu Y, Hou J, Liu Z, Yu H, Sun W, Xiong J, Liao Z, Zhou F, Xie C and Zhou Y: Gene therapy with tumor-specific promoter mediated suicide gene plus IL-12 gene enhanced tumor inhibition and prolonged host survival in a murine model of Lewis lung carcinoma. J Transl Med. 9(39)2011.PubMed/NCBI View Article : Google Scholar

37 

Fang L, Shanqu L, Ping G, Ting H, Xi W, Ke D, Min L, Junxia W and Huizhong Z: Gene therapy with RNAi targeting UHRF1 driven by tumor-specific promoter inhibits tumor growth and enhances the sensitivity of chemotherapeutic drug in breast cancer in vitro and in vivo. Cancer Chemother Pharmacol. 69:1079–1087. 2012.PubMed/NCBI View Article : Google Scholar

38 

Alekseenko IV, Pleshkan VV, Sass AV, Filyukova OB, Snezhkov EV and Sverdlov ED: A universal tumor-specific promoter for cancer gene therapy. Dokl Biochem Biophys. 480:158–161. 2018.PubMed/NCBI View Article : Google Scholar

39 

Liu Q, Kulak MV, Borcherding N, Maina PK, Zhang W, Weigel RJ and Qi HH: A novel HER2 gene body enhancer contributes to HER2 expression. Oncogene. 37:687–694. 2018.PubMed/NCBI View Article : Google Scholar

40 

Choe SS and Kim JB: Hypoxia-inducible factors: New strategies for treatment of obesity-induced metabolic diseases. Postgrad Med J. 96:451–452. 2020.PubMed/NCBI View Article : Google Scholar

41 

Shibata T, Giaccia AJ and Brown JM: Development of a hypoxia-responsive vector for tumor-specific gene therapy. Gene Ther. 7:493–498. 2000.PubMed/NCBI View Article : Google Scholar

42 

Zheng J, He S, Qi J, Wang X, Yu J, Wu Y, Gao Q, Wang K and Sun X: Targeted CDX2 expression inhibits aggressive phenotypes of colon cancer cells in vitro and in vivo. Int J Oncol. 51:478–488. 2017.PubMed/NCBI View Article : Google Scholar

43 

Zhu S, Ying Y, Ye J, Chen M, Wu Q, Dou H, Ni W, Xu H and Xu J: AAV2-mediated and hypoxia response element-directed expression of bFGF in neural stem cells showed therapeutic effects on spinal cord injury in rats. Cell Death Dis. 12(274)2021.PubMed/NCBI View Article : Google Scholar

44 

Zhu S, Ying Y, He Y, Zhong X, Ye J, Huang Z, Chen M, Wu Q, Zhang Y, Xiang Z, et al: Hypoxia response element-directed expression of bFGF in dental pulp stem cells improve the hypoxic environment by targeting pericytes in SCI rats. Bioact Mater. 6:2452–2466. 2021.PubMed/NCBI View Article : Google Scholar

45 

Yan M, Schwaederle M, Arguello D, Millis SZ, Gatalica Z and Kurzrock R: HER2 expression status in diverse cancers: Review of results from 37,992 patients. Cancer Metastasis Rev. 34:157–164. 2015.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
He S, Hou Y, Hou L, Chen N, Yang X, Wang H, Han P, Fan Y, Zhao J, Zhang J, Zhang J, et al: Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>. Exp Ther Med 25: 245, 2023.
APA
He, S., Hou, Y., Hou, L., Chen, N., Yang, X., Wang, H. ... Geng, J. (2023). Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>. Experimental and Therapeutic Medicine, 25, 245. https://doi.org/10.3892/etm.2023.11944
MLA
He, S., Hou, Y., Hou, L., Chen, N., Yang, X., Wang, H., Han, P., Fan, Y., Zhao, J., Zhang, J., Geng, J."Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>". Experimental and Therapeutic Medicine 25.6 (2023): 245.
Chicago
He, S., Hou, Y., Hou, L., Chen, N., Yang, X., Wang, H., Han, P., Fan, Y., Zhao, J., Zhang, J., Geng, J."Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>". Experimental and Therapeutic Medicine 25, no. 6 (2023): 245. https://doi.org/10.3892/etm.2023.11944
Copy and paste a formatted citation
x
Spandidos Publications style
He S, Hou Y, Hou L, Chen N, Yang X, Wang H, Han P, Fan Y, Zhao J, Zhang J, Zhang J, et al: Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>. Exp Ther Med 25: 245, 2023.
APA
He, S., Hou, Y., Hou, L., Chen, N., Yang, X., Wang, H. ... Geng, J. (2023). Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>. Experimental and Therapeutic Medicine, 25, 245. https://doi.org/10.3892/etm.2023.11944
MLA
He, S., Hou, Y., Hou, L., Chen, N., Yang, X., Wang, H., Han, P., Fan, Y., Zhao, J., Zhang, J., Geng, J."Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>". Experimental and Therapeutic Medicine 25.6 (2023): 245.
Chicago
He, S., Hou, Y., Hou, L., Chen, N., Yang, X., Wang, H., Han, P., Fan, Y., Zhao, J., Zhang, J., Geng, J."Targeted RASSF1A expression inhibits proliferation of HER2‑positive breast cancer cells <em>in vitro</em>". Experimental and Therapeutic Medicine 25, no. 6 (2023): 245. https://doi.org/10.3892/etm.2023.11944
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