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Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells

  • Authors:
    • Fangrui Dong
    • Pengfei Zhang
    • Bin Ma
    • Guangjie Bao
    • Hong Kang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Key Laboratory of Stomatology of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou, Gansu 730030, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 387
    |
    Published online on: June 29, 2023
       https://doi.org/10.3892/etm.2023.12086
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Abstract

Temporomandibular joint (TMJ) disc degeneration is a common disease characterized by a decrease in metabolic function. The present study aimed to investigate the pathogenesis of TMJ disc degeneration by analyzing the effects of oxygen and glucose concentrations on metabolism in a simulated TMJ disc cell growth environment. Cell samples were divided into 10 groups and cultured in different nutritional environments, including 21 and 2% O2 partial pressures and various glucose concentrations (0, 0.5, 3, 5.5 and 22.5 mmol/l). Cell proliferation, extracellular matrix content, mitochondrial function, and cell metabolism were subsequently analyzed. The results demonstrated that hypoxia and a low glucose concentration inhibited cell growth, and low glucose concentration inhibited extracellular matrix synthesis and adenosine 5'‑monophosphate‑activated protein kinase expression. Hypoxic conditions also induced a compensatory increase in the number of mitochondria, whereas mitochondrial deformation and swelling were observed in the absence of glucose. According to this study, the primary metabolic pathway of TMJ disc cells is glycolysis. It was concluded that hypoxic conditions and normal glucose concentrations are needed for the growth of TMJ disc cells. Glucose is necessary to ensure cell survival, extracellular matrix synthesis and mitochondrial function. Glucose deficiency may be related to disc degeneration, aging and disease mechanisms.
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Copy and paste a formatted citation
Spandidos Publications style
Dong F, Zhang P, Ma B, Bao G and Kang H: Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells. Exp Ther Med 26: 387, 2023.
APA
Dong, F., Zhang, P., Ma, B., Bao, G., & Kang, H. (2023). Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells. Experimental and Therapeutic Medicine, 26, 387. https://doi.org/10.3892/etm.2023.12086
MLA
Dong, F., Zhang, P., Ma, B., Bao, G., Kang, H."Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells". Experimental and Therapeutic Medicine 26.2 (2023): 387.
Chicago
Dong, F., Zhang, P., Ma, B., Bao, G., Kang, H."Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells". Experimental and Therapeutic Medicine 26, no. 2 (2023): 387. https://doi.org/10.3892/etm.2023.12086
Copy and paste a formatted citation
x
Spandidos Publications style
Dong F, Zhang P, Ma B, Bao G and Kang H: Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells. Exp Ther Med 26: 387, 2023.
APA
Dong, F., Zhang, P., Ma, B., Bao, G., & Kang, H. (2023). Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells. Experimental and Therapeutic Medicine, 26, 387. https://doi.org/10.3892/etm.2023.12086
MLA
Dong, F., Zhang, P., Ma, B., Bao, G., Kang, H."Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells". Experimental and Therapeutic Medicine 26.2 (2023): 387.
Chicago
Dong, F., Zhang, P., Ma, B., Bao, G., Kang, H."Effects of glucose concentration and oxygen partial pressure on the respiratory metabolism of sheep temporomandibular joint disc cells". Experimental and Therapeutic Medicine 26, no. 2 (2023): 387. https://doi.org/10.3892/etm.2023.12086
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