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Association between the presence and genotype of Helicobacter pylori and periodontitis

  • Authors:
    • Rong Li
    • Yuxiao Luo
    • Qin Dong
    • Yuqing Yin
    • Yiwei Ma
    • Jiayu Pan
    • Yaping Pan
    • Dongmei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Periodontology, Shenzhen Stomatological Hospital, Southern Medical University, Shenzhen, Guangdong 518000, P.R. China, Department of Periodontology, School of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Disease, Shenyang, Liaoning 110002, P.R. China, Department of Periodontics and Oral Biology, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Disease, Shenyang, Liaoning 110002, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 489
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    Published online on: September 4, 2023
       https://doi.org/10.3892/etm.2023.12188
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Abstract

Whether Helicobacter pylori (H. pylori) infection is associated with periodontitis has been contested for decades. The relationship between H. pylori genotypes and periodontitis has not been clarified either. The present study provides a novel perspective to better understand the role of H. pylori in the pathogenesis of periodontitis. A total of 53 volunteers were recruited and divided into 3 groups in this cross‑sectional study, namely the periodontally healthy group (15 participants), the stage I/II periodontitis group (20 participants) and the stage III/IV periodontitis group (18 participants). DNA from the subgingival plaque of all participants was extracted and PCR was performed using specific primers for the urease C gene and cytotoxin‑associated gene A (cagA)/vacuolating cytotoxin gene A (vacA) to detect the presence and genotype of H. pylori. A χ2 test and one‑way ANOVA were performed on the data. There was no significant difference in sex, age or body mass index between the groups. The detection rate of H. pylori was 39.62% in the total population and increased with the deepening of probing depth and clinical attachment loss. There were significant differences in the detection rate of H. pylori among the three groups, with 13.33, 40.00 and 61.11% in the periodontally healthy, stage I/II periodontitis and stage III/IV periodontitis groups, respectively (χ2=8.760, P<0.001). The cagA‑/vacAs2m2 genotype was most commonly detected in the periodontally healthy group (100%). In the periodontitis group, cagA+/vacAs1m2 was the most commonly detected genotype in the stage I/II periodontitis group (37.5%) and cagA+/vacAs1m1 in the stage III/IV periodontitis group (36.3%). The results of the present study suggest that the detection rates and genotypes of H. pylori in the subgingival plaque are associated with the status of periodontitis. cagA+/vacAs1m1 and cagA+/vacAs1m2 may be considered virulence markers of periodontitis. However, given the small sample size and lack of correlation analysis of the study, further larger scale and high‑quality clinical trials are required to confirm these findings.
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Copy and paste a formatted citation
Spandidos Publications style
Li R, Luo Y, Dong Q, Yin Y, Ma Y, Pan J, Pan Y and Zhang D: Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis. Exp Ther Med 26: 489, 2023.
APA
Li, R., Luo, Y., Dong, Q., Yin, Y., Ma, Y., Pan, J. ... Zhang, D. (2023). Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis. Experimental and Therapeutic Medicine, 26, 489. https://doi.org/10.3892/etm.2023.12188
MLA
Li, R., Luo, Y., Dong, Q., Yin, Y., Ma, Y., Pan, J., Pan, Y., Zhang, D."Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis". Experimental and Therapeutic Medicine 26.4 (2023): 489.
Chicago
Li, R., Luo, Y., Dong, Q., Yin, Y., Ma, Y., Pan, J., Pan, Y., Zhang, D."Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis". Experimental and Therapeutic Medicine 26, no. 4 (2023): 489. https://doi.org/10.3892/etm.2023.12188
Copy and paste a formatted citation
x
Spandidos Publications style
Li R, Luo Y, Dong Q, Yin Y, Ma Y, Pan J, Pan Y and Zhang D: Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis. Exp Ther Med 26: 489, 2023.
APA
Li, R., Luo, Y., Dong, Q., Yin, Y., Ma, Y., Pan, J. ... Zhang, D. (2023). Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis. Experimental and Therapeutic Medicine, 26, 489. https://doi.org/10.3892/etm.2023.12188
MLA
Li, R., Luo, Y., Dong, Q., Yin, Y., Ma, Y., Pan, J., Pan, Y., Zhang, D."Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis". Experimental and Therapeutic Medicine 26.4 (2023): 489.
Chicago
Li, R., Luo, Y., Dong, Q., Yin, Y., Ma, Y., Pan, J., Pan, Y., Zhang, D."Association between the presence and genotype of <em>Helicobacter pylori</em> and periodontitis". Experimental and Therapeutic Medicine 26, no. 4 (2023): 489. https://doi.org/10.3892/etm.2023.12188
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