Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
February-2024 Volume 27 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2024 Volume 27 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data1.pdf
    • Supplementary_Data2.xlsx
    • Supplementary_Data3.xlsx
    • Supplementary_Data4.xlsx
    • Supplementary_Data5.xlsx
    • Supplementary_Data6.xlsx
Article Open Access

HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis

  • Authors:
    • Min Wang
    • Yuling Hu
    • Feng Cai
    • Jiayong Qiu
    • Yimin Mao
    • Yingmin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 86
    |
    Published online on: January 8, 2024
       https://doi.org/10.3892/etm.2024.12375
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Invasive aspergillosis (IA) is a severe disease, the pathogenesis of which remains unclear. The present study aimed to determine the molecular mechanism of IA and to identify potential biomarkers using bioinformatics analysis. The GSE78000 dataset, which includes data from patients with IA and healthy individuals, was downloaded from Gene Expression Omnibus. Differentially expressed genes (DEGs) between the IA and control groups were identified with the ‘affy’ package in R software. The Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) databases were then used to analyse the function and pathway enrichment of DEGs. The protein‑protein interaction network was analysed with the Search Tool for the Retrieval of Interacting Genes (STRING) website. In addition, DEGs were confirmed using reverse transcription‑quantitative PCR and western blotting in samples with IA (n=6) and control samples (n=6) collected from the Department of Respiratory and Critical Care Medicine of the First Affiliated Hospital of Henan University of Science and Technology (Luoyang, China). The present study identified 735 DEGs, including 312 upregulated and 423 downregulated genes. Through GO and KEGG analyses of the DEGs, macrophage activation and hypoxia‑inducible factor 1 (HIF‑1) signalling pathways were revealed to be significantly upregulated and downregulated, respectively, in patients with IA compared with that of the healthy individuals. Subsequently, correlation analysis of macrophage activation and HIF‑1 signalling pathways was revealed using correlation as a distance metric for hierarchical clustering correlation analysis. However, there was no protein‑protein interaction between the macrophage activity regulation and HIF‑1 signalling pathways based on STRING analysis. In summary, the present study identified candidate genes and associated molecules that may be associated to IA and revealed potential biomarkers and therapeutic targets for IA.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Park HS, Jun SC, Han KH, Hong SB and Yu JH: Diversity, application, and synthetic biology of industrially important aspergillus fungi. Adv Appl Microbiol. 100:161–202. 2017.PubMed/NCBI View Article : Google Scholar

2 

Becker KL, Ifrim DC, Quintin J, Netea MG and van de Veerdonk FL: Antifungal innate immunity: Recognition and inflammatory networks. Semin Immunopathol. 37:107–116. 2015.PubMed/NCBI View Article : Google Scholar

3 

Takakura S: Zygomycosis. Nihon Rinsho. 66:2356–2361. 2008.PubMed/NCBI(In Japanese).

4 

Cadena J, Thompson GR III and Patterson TF: Invasive aspergillosis: Current strategies for diagnosis and management. Infect Dis Clin North Am. 30:125–142. 2016.PubMed/NCBI View Article : Google Scholar

5 

Cadena J, Thompson GR III and Patterson TF: Aspergillosis: Epidemiology, diagnosis, and treatment. Infect Dis Clin North Am. 35:415–434. 2021.PubMed/NCBI View Article : Google Scholar

6 

Latge JP and Chamilos G: Aspergillus fumigatus and aspergillosis in 2019. Clin Microbiol Rev. 33:e00140–18. 2019.PubMed/NCBI View Article : Google Scholar

7 

Oren I and Goldstein N: Invasive pulmonary aspergillosis. Curr Opin Pulm Med. 8:195–200. 2002.PubMed/NCBI View Article : Google Scholar

8 

Darling BA and Milder EA: Invasive aspergillosis. Pediatr Rev. 39:476–478. 2018.PubMed/NCBI View Article : Google Scholar

9 

Verma N, Singh S, Syal A, Pradhan P and Singh M and Singh M: Invasive aspergillosis is a critical determinant of mortality in cirrhosis: A systematic review with meta-analysis. Med Mycol. 59:1092–1100. 2021.PubMed/NCBI View Article : Google Scholar

10 

Arvanitis M and Mylonakis E: Diagnosis of invasive aspergillosis: Recent developments and ongoing challenges. Eur J Clin Invest. 45:646–652. 2015.PubMed/NCBI View Article : Google Scholar

11 

Dhesi Z, Herbst S and Armstrong-James D: Transcript profiling of the murine immune response to invasive aspergillosis. Methods Mol Biol. 845:435–444. 2012.PubMed/NCBI View Article : Google Scholar

12 

Wang M, Zhang M, Qiu J, Liu C, Lou Y, Wang T, Zhang Y and Mao Y: PU.1-CD23 signaling mediates pulmonary innate immunity against Aspergillus fumigatus infection by driving inflammatory response. BMC Immunol. 24(4)2023.PubMed/NCBI View Article : Google Scholar

13 

Fliesser M, Morton CO, Bonin M, Ebel F, Hünniger K, Kurzai O, Einsele H and Löffler J: Hypoxia-inducible factor 1α modulates metabolic activity and cytokine release in anti-Aspergillus fumigatus immune responses initiated by human dendritic cells. Int J Med Microbiol. 305:865–873. 2015.PubMed/NCBI View Article : Google Scholar

14 

Morris NL, Michael DN, Crotty KM, Chang SS and Yeligar SM: Alcohol-Induced glycolytic shift in alveolar macrophages is mediated by hypoxia-inducible factor-1 Alpha. Front Immunol. 13(865492)2022.PubMed/NCBI View Article : Google Scholar

15 

Dix A, Czakai K, Springer J, Fliesser M, Bonin M, Guthke R, Schmitt AL, Einsele H, Linde J and Löffler J: Genome-Wide expression profiling reveals S100B as biomarker for invasive aspergillosis. Front Microbiol. 7(320)2016.PubMed/NCBI View Article : Google Scholar

16 

Wang Y, Sun FZ, Lin W and Zhang SQ: AC-PCoA: Adjustment for confounding factors using principal coordinate analysis. PLoS Comput Biol. 18(e1010184)2022.PubMed/NCBI View Article : Google Scholar

17 

Delbeke H, Casteels I and Joossens M: DNA extraction protocol impacts ocular surface microbiome profile. Front Microbiol. 14(1128917)2023.PubMed/NCBI View Article : Google Scholar

18 

Zhan Z, Chen Y, Duan Y, Li L, Mew K, Hu P, Ren H and Peng M: Identification of key genes, pathways and potential therapeutic agents for liver fibrosis using an integrated bioinformatics analysis. PeerJ. 7(e6645)2019.PubMed/NCBI View Article : Google Scholar

19 

Haldermans P, Shkedy Z, Van Sanden S, Burzykowski T and Aerts M: Using linear mixed models for normalization of cDNA microarrays. Stat Appl Genet Mol Biol. 6(Article 19)2007.PubMed/NCBI View Article : Google Scholar

20 

Ding J and Zhang Y: Analysis of key GO terms and KEGG pathways associated with carcinogenic chemicals. Comb Chem High Throughput Screen: Dec 18, 2017 (Epub ahead of print).

21 

Chen L, Zhang YH, Lu G, Huang T and Cai YD: Analysis of cancer-related lncRNAs using gene ontology and KEGG pathways. Artif Intell Med. 76:27–36. 2017.PubMed/NCBI View Article : Google Scholar

22 

Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC and Lempicki RA: DAVID: Database for annotation, visualization, and integrated discovery. Genome Biol. 4(P3)2003.PubMed/NCBI

23 

Goutzelas Y, Kontou P, Mamuris Z, Bagos P and Sarafidou T: Meta-analysis of gene expression data in adipose tissue reveals new obesity associated genes. Gene. 818(146223)2022.PubMed/NCBI View Article : Google Scholar

24 

Wallach T, Schellenberg K, Maier B, Kalathur RK, Porras P, Wanker EE, Futschik ME and Kramer A: Dynamic circadian protein-protein interaction networks predict temporal organization of cellular functions. PLoS Genet. 9(e1003398)2013.PubMed/NCBI View Article : Google Scholar

25 

Szklarczyk D, Gable AL, Nastou KC, Lyon D, Kirsch R, Pyysalo S, Doncheva NT, Legeay M, Fang T, Bork P, et al: The STRING database in 2021: Customizable protein-protein networks, and functional characterization of user-uploaded gene/measurement sets. Nucleic Acids Res. 49(D1):D605–D612. 2021.PubMed/NCBI View Article : Google Scholar

26 

Liu J, Zhou S, Li S, Jiang Y, Wan Y, Ma X and Cheng W: Eleven genes associated with progression and prognosis of endometrial cancer (EC) identified by comprehensive bioinformatics analysis. Cancer Cell Int. 19(136)2019.PubMed/NCBI View Article : Google Scholar

27 

Chen Y, Li H, Lai L, Feng Q and Shen J: Identification of common differentially expressed genes and potential therapeutic targets in ulcerative colitis and rheumatoid arthritis. Front Genet. 11(572194)2020.PubMed/NCBI View Article : Google Scholar

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

29 

Johnson G, Ferrini A, Dolan SK, Nolan T, Agrawal S, Doyle S and Bustin SA: Biomarkers for invasive aspergillosis: The challenges continue. Biomark Med. 8:429–451. 2014.PubMed/NCBI View Article : Google Scholar

30 

Margalit A and Kavanagh K: The innate immune response to aspergillus fumigatus at the alveolar surface. FEMS Microbiol Rev. 39:670–687. 2015.PubMed/NCBI View Article : Google Scholar

31 

Seifert M, Nairz M, Schroll A, Schrettl M, Haas H and Weiss G: Effects of the aspergillus fumigatus siderophore systems on the regulation of macrophage immune effector pathways and iron homeostasis. Immunobiology. 213:767–778. 2008.PubMed/NCBI View Article : Google Scholar

32 

Heinekamp T, Schmidt H, Lapp K, Pahtz V, Shopova I, Koster-Eiserfunke N, Kruger T, Kniemeyer O and Brakhage AA: Interference of aspergillus fumigatus with the immune response. Semin Immunopathol. 37:141–152. 2015.PubMed/NCBI View Article : Google Scholar

33 

Tan HY, Wang N, Zhang C, Chan YT, Yuen MF and Feng Y: Lysyl Oxidase-Like 4 Fosters an immunosuppressive microenvironment during hepatocarcinogenesis. Hepatology. 73:2326–2341. 2021.PubMed/NCBI View Article : Google Scholar

34 

Fecher RA, Horwath MC, Friedrich D, Rupp J and Deepe GS Jr: Inverse correlation between IL-10 and HIF-1alpha in macrophages infected with histoplasma capsulatum. J Immunol. 197:565–579. 2016.PubMed/NCBI View Article : Google Scholar

35 

Goncalves SM, Duarte-Oliveira C, Campos CF, Aimanianda V, Ter Horst R, Leite L, Mercier T, Pereira P, Fernandez-Garcia M, Antunes D, et al: Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity. Nat Commun. 11(2282)2020.PubMed/NCBI View Article : Google Scholar

36 

Cheng Y, Feng Y, Xia Z, Li X and Rong J: ω-Alkynyl arachidonic acid promotes anti-inflammatory macrophage M2 polarization against acute myocardial infarction via regulating the cross-talk between PKM2, HIF-1α and iNOS. Biochim Biophys Acta Mol Cell Biol Lipids. 1862:1595–1605. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang M, Hu Y, Cai F, Qiu J, Mao Y and Zhang Y: HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis. Exp Ther Med 27: 86, 2024.
APA
Wang, M., Hu, Y., Cai, F., Qiu, J., Mao, Y., & Zhang, Y. (2024). HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis. Experimental and Therapeutic Medicine, 27, 86. https://doi.org/10.3892/etm.2024.12375
MLA
Wang, M., Hu, Y., Cai, F., Qiu, J., Mao, Y., Zhang, Y."HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis". Experimental and Therapeutic Medicine 27.2 (2024): 86.
Chicago
Wang, M., Hu, Y., Cai, F., Qiu, J., Mao, Y., Zhang, Y."HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis". Experimental and Therapeutic Medicine 27, no. 2 (2024): 86. https://doi.org/10.3892/etm.2024.12375
Copy and paste a formatted citation
x
Spandidos Publications style
Wang M, Hu Y, Cai F, Qiu J, Mao Y and Zhang Y: HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis. Exp Ther Med 27: 86, 2024.
APA
Wang, M., Hu, Y., Cai, F., Qiu, J., Mao, Y., & Zhang, Y. (2024). HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis. Experimental and Therapeutic Medicine, 27, 86. https://doi.org/10.3892/etm.2024.12375
MLA
Wang, M., Hu, Y., Cai, F., Qiu, J., Mao, Y., Zhang, Y."HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis". Experimental and Therapeutic Medicine 27.2 (2024): 86.
Chicago
Wang, M., Hu, Y., Cai, F., Qiu, J., Mao, Y., Zhang, Y."HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis". Experimental and Therapeutic Medicine 27, no. 2 (2024): 86. https://doi.org/10.3892/etm.2024.12375
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team