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Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors

  • Authors:
    • Zhongxin Sun
    • Michika Fukui
    • Shigeru Taketani
    • Ayako Kako
    • Sakurako Kunieda
    • Natsuko Kakudo
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 156
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    Published online on: February 21, 2024
       https://doi.org/10.3892/etm.2024.12444
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Abstract

Human adipose‑derived stem cells (hASCs) play important roles in regenerative medicine and tissue engineering. However, their clinical applications are limited because of their instability during cell culture. Platelet lysates (PLTs) contain large amounts of growth factors that are useful for manufacturing cellular products. Platelet‑derived growth factor (PDGF) is a major growth factor in PLTs and a potent mitogen in hASCs. To optimize growth conditions, the effects of a combination of growth factors on the promotion of hASC proliferation were investigated. Moreover, PDGF‑BB combined with vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) markedly enhanced the viability of hASCs compared with the effects of PDGF‑BB alone. Neither VEGF nor HGF had any effect alone. All growth factor receptor inhibitors inhibited cell proliferation. Wound healing assays revealed that VEGF and HGF stimulated PDGF‑dependent cell migration. The effects of these growth factors on the activation of their cognate receptors and signaling enzymes were assessed using immunoblotting. Phosphorylation of PDGF receptor (PDGFR)β, VEGF receptor (VEGFR)2 and MET proto‑oncogene and receptor tyrosine kinase was induced by PDGF‑BB treatment, and was further increased by treatment with PDGF‑BB/VEGF and PDGF‑BB/HGF. The levels of phospho‑ERK1/2 and phospho‑p38MAPK were increased by these treatments in parallel. Furthermore, the expression levels of SRY‑box transcription factor 2 and peroxisome proliferator‑activated receptor g were increased in PDGF‑BB‑treated cells, and PDGF‑BB played a dominant role in spheroid formation. The findings of the present study highlighted that PDGF/PDGFR signaling played a predominant role in the proliferation and migration of hASCs, and suggested that PDGF was responsible for the efficacy of other growth factors when hASCs were cultured with PLTs.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Z, Fukui M, Taketani S, Kako A, Kunieda S and Kakudo N: Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors. Exp Ther Med 27: 156, 2024.
APA
Sun, Z., Fukui, M., Taketani, S., Kako, A., Kunieda, S., & Kakudo, N. (2024). Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors. Experimental and Therapeutic Medicine, 27, 156. https://doi.org/10.3892/etm.2024.12444
MLA
Sun, Z., Fukui, M., Taketani, S., Kako, A., Kunieda, S., Kakudo, N."Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors". Experimental and Therapeutic Medicine 27.4 (2024): 156.
Chicago
Sun, Z., Fukui, M., Taketani, S., Kako, A., Kunieda, S., Kakudo, N."Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors". Experimental and Therapeutic Medicine 27, no. 4 (2024): 156. https://doi.org/10.3892/etm.2024.12444
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Spandidos Publications style
Sun Z, Fukui M, Taketani S, Kako A, Kunieda S and Kakudo N: Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors. Exp Ther Med 27: 156, 2024.
APA
Sun, Z., Fukui, M., Taketani, S., Kako, A., Kunieda, S., & Kakudo, N. (2024). Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors. Experimental and Therapeutic Medicine, 27, 156. https://doi.org/10.3892/etm.2024.12444
MLA
Sun, Z., Fukui, M., Taketani, S., Kako, A., Kunieda, S., Kakudo, N."Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors". Experimental and Therapeutic Medicine 27.4 (2024): 156.
Chicago
Sun, Z., Fukui, M., Taketani, S., Kako, A., Kunieda, S., Kakudo, N."Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose‑derived stem cells under culture conditions with a combination of growth factors". Experimental and Therapeutic Medicine 27, no. 4 (2024): 156. https://doi.org/10.3892/etm.2024.12444
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