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Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress

  • Authors:
    • Chen Yuan
    • Heng Yang
    • Wanqi Lan
    • Juesheng Yang
    • Yanhua Tang
  • View Affiliations / Copyright

    Affiliations: The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 187
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    Published online on: March 7, 2024
       https://doi.org/10.3892/etm.2024.12475
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Abstract

Nicotinamide riboside (NR) has been reported to play a protective role in myocardial ischemia‑reperfusion (I/R) injury when used in association with other drugs; however, the individual effect of NR is unknown. In the present study Evan's blue/triphenyl tetrazolium chloride staining, hematoxylin and eosin staining, echocardiography, western blotting, reverse transcription‑quantitative PCR, and the detection of myocardial injury‑associated markers and oxidative stress metabolites were used to explore the ability of NR to alleviate cardiac I/R injury and the relevant mechanisms of action. In a mouse model of I/R injury, dietary supplementation with NR reduced the area of myocardial ischemic infarction, alleviated pathological myocardial changes, decreased inflammatory cell infiltration and attenuated the levels of mitochondrial reactive oxygen species (ROS) and creatine kinase myocardial band (CK‑MB). In addition, echocardiography suggested that NR alleviated the functional damage of the myocardium caused by I/R injury. In H9c2 cells, NR pretreatment reduced the levels of lactate dehydrogenase, CK‑MB, malondialdehyde, superoxide dismutase and ROS, and reduced cell mortality after the induction of hypoxia/reoxygenation (H/R) injury. In addition, the results indicated NR activated sirt 1 via the upregulation of nicotinamide adenine dinucleotide (NAD+) and protected the cells against autophagy. The sirt 1 inhibitor EX527 significantly attenuated the ability of NR to inhibit autophagy, but had no significant effect on the ROS content of the H9c2 cells. In summary, the present study suggests that NR protects against autophagy by increasing the NAD+ content in the body via the sirt 1 pathway, although the sirt 1 pathway does not affect oxidative stress.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan C, Yang H, Lan W, Yang J and Tang Y: Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress. Exp Ther Med 27: 187, 2024.
APA
Yuan, C., Yang, H., Lan, W., Yang, J., & Tang, Y. (2024). Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress. Experimental and Therapeutic Medicine, 27, 187. https://doi.org/10.3892/etm.2024.12475
MLA
Yuan, C., Yang, H., Lan, W., Yang, J., Tang, Y."Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress". Experimental and Therapeutic Medicine 27.5 (2024): 187.
Chicago
Yuan, C., Yang, H., Lan, W., Yang, J., Tang, Y."Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress". Experimental and Therapeutic Medicine 27, no. 5 (2024): 187. https://doi.org/10.3892/etm.2024.12475
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan C, Yang H, Lan W, Yang J and Tang Y: Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress. Exp Ther Med 27: 187, 2024.
APA
Yuan, C., Yang, H., Lan, W., Yang, J., & Tang, Y. (2024). Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress. Experimental and Therapeutic Medicine, 27, 187. https://doi.org/10.3892/etm.2024.12475
MLA
Yuan, C., Yang, H., Lan, W., Yang, J., Tang, Y."Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress". Experimental and Therapeutic Medicine 27.5 (2024): 187.
Chicago
Yuan, C., Yang, H., Lan, W., Yang, J., Tang, Y."Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress". Experimental and Therapeutic Medicine 27, no. 5 (2024): 187. https://doi.org/10.3892/etm.2024.12475
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