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Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells

  • Authors:
    • Hanui Lee
    • Gyeong Han Jeong
    • So-Yeun Woo
    • Hyo Jin Choi
    • Byung Yeoup Chung
    • Kyung-Bon Lee
    • Hyoung-Woo Bai
  • View Affiliations / Copyright

    Affiliations: Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup, Jeollabuk 56212, Republic of Korea, Medical Device Development Center, Daegu‑Gyeongbuk Medical Innovation Foundation (K‑MEDI Hub), Daegu 41061, Republic of Korea, Department of Biology Education, Chonnam National University, Gwangju 61186, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 192
    |
    Published online on: March 11, 2024
       https://doi.org/10.3892/etm.2024.12480
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Abstract

Nialamide is a non‑selective monoamine oxidase inhibitor that was widely used as an antidepressant. However, it has been prohibited for decades in the depressive medicine market due to the adverse hepatotoxic side effects. The re‑use of drugs that have been withdrawn from the market represents a promising approach for the development of novel incrementally modified drugs and, in this context, ionizing radiation can serve as a powerful tool for producing new drug candidates. The present study exposed nialamide to γ radiation at 50 kGy to obtain the novel cyclized benzylamide, nialaminosin (compound 2), along with five known compounds, 3‑amino‑N‑benzylpropanamide (compound 3), 3‑methoxy‑N‑benzylpropanamide (compound 4), 3‑hydroxy‑N‑benzylpropanamide (HBPA; compound 5), N‑benzylpropanamide (compound 6) and isonicotinamide (compound 7). Among the isolated compounds, HBPA was established to inhibit the lipopolysaccharide‑induced overproduction of pro‑inflammatory mediators, including nitric oxide (NO) and prostaglandin E2 and cytokines including TNF‑α, IL‑6 and IL‑10, without causing cytotoxicity to both RAW 264.7 and DH82 cells. Furthermore, HBPA was found to reduce the protein expression of inducible NO synthase and cyclooxygenase‑2 in macrophages and compared with nialamide, it was established to have more potent radical scavenging activity. The present study therefore suggested the application of HBPA for the improvement of anti‑inflammatory properties using ionizing radiation technology on the withdrawn drug nialamide.
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Copy and paste a formatted citation
Spandidos Publications style
Lee H, Jeong GH, Woo S, Choi HJ, Chung BY, Lee K and Bai H: Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells. Exp Ther Med 27: 192, 2024.
APA
Lee, H., Jeong, G.H., Woo, S., Choi, H.J., Chung, B.Y., Lee, K., & Bai, H. (2024). Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells. Experimental and Therapeutic Medicine, 27, 192. https://doi.org/10.3892/etm.2024.12480
MLA
Lee, H., Jeong, G. H., Woo, S., Choi, H. J., Chung, B. Y., Lee, K., Bai, H."Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells". Experimental and Therapeutic Medicine 27.5 (2024): 192.
Chicago
Lee, H., Jeong, G. H., Woo, S., Choi, H. J., Chung, B. Y., Lee, K., Bai, H."Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells". Experimental and Therapeutic Medicine 27, no. 5 (2024): 192. https://doi.org/10.3892/etm.2024.12480
Copy and paste a formatted citation
x
Spandidos Publications style
Lee H, Jeong GH, Woo S, Choi HJ, Chung BY, Lee K and Bai H: Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells. Exp Ther Med 27: 192, 2024.
APA
Lee, H., Jeong, G.H., Woo, S., Choi, H.J., Chung, B.Y., Lee, K., & Bai, H. (2024). Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells. Experimental and Therapeutic Medicine, 27, 192. https://doi.org/10.3892/etm.2024.12480
MLA
Lee, H., Jeong, G. H., Woo, S., Choi, H. J., Chung, B. Y., Lee, K., Bai, H."Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells". Experimental and Therapeutic Medicine 27.5 (2024): 192.
Chicago
Lee, H., Jeong, G. H., Woo, S., Choi, H. J., Chung, B. Y., Lee, K., Bai, H."Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cells". Experimental and Therapeutic Medicine 27, no. 5 (2024): 192. https://doi.org/10.3892/etm.2024.12480
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