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Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study

  • Authors:
    • Maria Trandafir
    • Gratiela Gradisteanu Pircalabioru
    • Octavian Savu
  • View Affiliations / Copyright

    Affiliations: Doctoral School, ‘Carol Davila’ University of Medicine and Pharmacy, 050474 Bucharest, Romania, Earth, Environmental and Life Sciences Division, Research Institute of University of Bucharest, 050095 Bucharest, Romania
    Copyright: © Trandafir et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 211
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    Published online on: March 20, 2024
       https://doi.org/10.3892/etm.2024.12500
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Abstract

Chronic kidney disease (CKD) is a widespread health concern, which affects ~9.1% of the global population and 12‑15% of individuals in upper‑middle income countries. Notably, ~2% of patients with CKD progress to end‑stage renal disease (ESRD), which leads to a substantial decline in the quality of life, an increased risk of mortality and significant financial burden. Patients with ESRD often still suffer from uremia and uremic syndromes, due to the accumulation of toxins between dialysis sessions and the inadequate removal of protein‑bound toxins during dialysis. A number of these toxins are produced by the gut microbiota through the fermentation of dietary proteins or cholines. Furthermore, the gut microbial community serves a key role in maintaining metabolic and immune equilibrium in individuals. The present study aimed to investigate the gut microbiota patterns in individuals with type 2 diabetes mellitus (T2DM) and ESRD via quantitative PCR analysis of the 16S and 18S ribosomal RNA of selected members of the gut microbiota. Individuals affected by both T2DM and ESRD displayed distinctive features within their intestinal microbiota. Specifically, there were increased levels of Gammaproteobacteria observed in these patients, and all subjects exhibited a notably increased presence of Enterobacteriaceae compared with healthy individuals. This particular microbial community has established connections with the presence of inflammatory processes in the colon. Moreover, the elevated levels of Enterobacteriaceae may serve as an indicator of an imbalance in the intestinal microbiota, a condition known as dysbiosis. In addition, the Betaproteobacteria phylum was significantly more prevalent in the stool samples of patients with both T2DM and ESRD when compared with the control group. In conclusion, the present pilot study focused on gut microbiome alterations in T2DM and ESRD. Understanding the relationship between dysbiosis and CKD may identify new areas of research and therapeutic interventions aimed at modulating the gut microbiota to improve the health and outcomes of individuals with CKD and ESRD.
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Copy and paste a formatted citation
Spandidos Publications style
Trandafir M, Pircalabioru GG and Savu O: Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study. Exp Ther Med 27: 211, 2024.
APA
Trandafir, M., Pircalabioru, G.G., & Savu, O. (2024). Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study. Experimental and Therapeutic Medicine, 27, 211. https://doi.org/10.3892/etm.2024.12500
MLA
Trandafir, M., Pircalabioru, G. G., Savu, O."Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study". Experimental and Therapeutic Medicine 27.5 (2024): 211.
Chicago
Trandafir, M., Pircalabioru, G. G., Savu, O."Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study". Experimental and Therapeutic Medicine 27, no. 5 (2024): 211. https://doi.org/10.3892/etm.2024.12500
Copy and paste a formatted citation
x
Spandidos Publications style
Trandafir M, Pircalabioru GG and Savu O: Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study. Exp Ther Med 27: 211, 2024.
APA
Trandafir, M., Pircalabioru, G.G., & Savu, O. (2024). Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study. Experimental and Therapeutic Medicine, 27, 211. https://doi.org/10.3892/etm.2024.12500
MLA
Trandafir, M., Pircalabioru, G. G., Savu, O."Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study". Experimental and Therapeutic Medicine 27.5 (2024): 211.
Chicago
Trandafir, M., Pircalabioru, G. G., Savu, O."Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study". Experimental and Therapeutic Medicine 27, no. 5 (2024): 211. https://doi.org/10.3892/etm.2024.12500
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