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Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1

  • Authors:
    • Xiaohua Gu
    • Zeyu Liu
    • Shan Shan
    • Tao Ren
    • Shaoyang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Xuhui, Shanghai 200233, P.R. China
    Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 219
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    Published online on: March 21, 2024
       https://doi.org/10.3892/etm.2024.12507
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Abstract

Disruption of the epithelial‑mesenchymal transition (EMT) of activated lung cells is an important strategy to inhibit the progression of idiopathic pulmonary fibrosis (IPF). The present study investigated the role of exosomes derived from airway basal cells on EMT of lung cells and elucidate the underlying mechanism. Exosomes were characterized by nanoparticle tracking analysis, transmission electron microscopy imaging and markers detection. The role of exosome on the EMT of lung epithelial cells and lung fibroblasts induced by TGF‑β1 was detected. RNA sequencing screened dysregulated genes in exosome‑treated group, followed by the bioinformatical analysis. One of the candidates, anoctamin‑1 (ANO1), was selected for further gain‑and‑loss phenotype assays. A bleomycin‑induced pulmonary fibrosis model was used to evaluate the treatment effect of exosomes. Exosomes were round‑like and positively expressed CD63 and tumor susceptibility gene 101 protein. Treatment with exosomes inhibited the EMT of lung cells activated by TGF‑β1. 4158 dysregulated genes were identified in exosome‑treated group under the threshold of |log2 fold‑change| value >1 and they were involved in the metabolism of various molecules, as well as motility‑related biological processes. A key gene, ANO1, was verified by reverse transcription‑quantitative PCR, whose overexpression induced the EMT of lung cells. By contrast, ANO1 knockdown reversed the EMT induced by TGF‑β1. In vivo assay indicated that exosome treatment ameliorated pulmonary fibrosis and inhibited the upregulation of ANO1 induced by bleomycin. In conclusion, airway basal cell‑derived exosomes suppressed the EMT of lung cells via the downregulation of ANO1. These exosomes represent a potential therapeutic option for patients with IPF.
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Copy and paste a formatted citation
Spandidos Publications style
Gu X, Liu Z, Shan S, Ren T and Wang S: Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1. Exp Ther Med 27: 219, 2024.
APA
Gu, X., Liu, Z., Shan, S., Ren, T., & Wang, S. (2024). Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1. Experimental and Therapeutic Medicine, 27, 219. https://doi.org/10.3892/etm.2024.12507
MLA
Gu, X., Liu, Z., Shan, S., Ren, T., Wang, S."Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1". Experimental and Therapeutic Medicine 27.5 (2024): 219.
Chicago
Gu, X., Liu, Z., Shan, S., Ren, T., Wang, S."Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1". Experimental and Therapeutic Medicine 27, no. 5 (2024): 219. https://doi.org/10.3892/etm.2024.12507
Copy and paste a formatted citation
x
Spandidos Publications style
Gu X, Liu Z, Shan S, Ren T and Wang S: Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1. Exp Ther Med 27: 219, 2024.
APA
Gu, X., Liu, Z., Shan, S., Ren, T., & Wang, S. (2024). Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1. Experimental and Therapeutic Medicine, 27, 219. https://doi.org/10.3892/etm.2024.12507
MLA
Gu, X., Liu, Z., Shan, S., Ren, T., Wang, S."Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1". Experimental and Therapeutic Medicine 27.5 (2024): 219.
Chicago
Gu, X., Liu, Z., Shan, S., Ren, T., Wang, S."Airway basal cell‑derived exosomes suppress epithelial‑mesenchymal transition of lung cells by inhibiting the expression of ANO1". Experimental and Therapeutic Medicine 27, no. 5 (2024): 219. https://doi.org/10.3892/etm.2024.12507
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