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Article Open Access

Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis

  • Authors:
    • Penglei Cui
    • Yanzhuo Zhang
    • Chao Wang
    • Bin Xiao
    • Qianqian Wang
    • Liang Zhang
    • Hongchao Li
    • Chengai Wu
    • Wei Tian
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, P.R. China, Department of Molecular Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, P.R. China, Department of Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, P.R. China, Department of Rheumatology and Immunology, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, P.R. China
    Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 237
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    Published online on: March 27, 2024
       https://doi.org/10.3892/etm.2024.12525
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Abstract

Long non‑coding RNAs (lncRNAs) have been previously researched in ankylosing spondylitis (AS). Nevertheless, there are few studies of lncRNAs and mRNAs associated with the pathogenesis of AS. Differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) between AS and normal samples were assessed using the R limma package. DOSE packages and ‘clusterProfiler’ were exploited for gene enrichment analysis. The functional association of proteins and protein interactions was assessed using the STRING database. To investigate the important genes and subnetworks in the protein‑protein interaction network, the MCODE plug‑in in the Cytoscape software was utilized. The gene mRNA was examined via reverse transcription‑quantitative PCR. In total, 152 DEmRNAs and 204 DElncRNAs were observed between normal and AS samples. A total of 68 candidate genes related to DElncRNA were identified. These candidate genes were enriched in 30 cellular component terms, 22 molecular functions, 83 biological processes, 9 Kyoto Encyclopedia of Genes and Genomes, and 36 disease ontology pathways. NONHSAG037054.2 was the most related lncRNA to genes, and GABPA was the most connected gene to lncRNA in AS. The NCBI/GenBank accession number of the lncRNA NONHSAG037054.2 was not found because it is not included in NCBI. The information of lncRNA NONHSAG037054.2 can be found at the website (http://www.noncode.org/show_gene.php?id=NONHSAG037054 and https://www.genecards.org/cgi‑bin/carddisp.pl?gene=ACAP2‑IT1). In total, 13 microRNAs (miRNAs) and 46 miRNAs associated with NONHSAG037054.2 and GABPA, respectively, were found. A total of 173 RNA‑binding protein genes were associated with both NONHSAG037054.2 and GABPA. In addition, GABPA was downregulated in AS samples, suggesting it may have diagnostic value in AS. In conclusion, NONHSAG037054.2 and GABPA are associated with AS. GABPA was downregulated in AS, and it could serve as a novel diagnostic factor for AS.
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Copy and paste a formatted citation
Spandidos Publications style
Cui P, Zhang Y, Wang C, Xiao B, Wang Q, Zhang L, Li H, Wu C and Tian W: Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis. Exp Ther Med 27: 237, 2024.
APA
Cui, P., Zhang, Y., Wang, C., Xiao, B., Wang, Q., Zhang, L. ... Tian, W. (2024). Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis. Experimental and Therapeutic Medicine, 27, 237. https://doi.org/10.3892/etm.2024.12525
MLA
Cui, P., Zhang, Y., Wang, C., Xiao, B., Wang, Q., Zhang, L., Li, H., Wu, C., Tian, W."Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis". Experimental and Therapeutic Medicine 27.5 (2024): 237.
Chicago
Cui, P., Zhang, Y., Wang, C., Xiao, B., Wang, Q., Zhang, L., Li, H., Wu, C., Tian, W."Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis". Experimental and Therapeutic Medicine 27, no. 5 (2024): 237. https://doi.org/10.3892/etm.2024.12525
Copy and paste a formatted citation
x
Spandidos Publications style
Cui P, Zhang Y, Wang C, Xiao B, Wang Q, Zhang L, Li H, Wu C and Tian W: Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis. Exp Ther Med 27: 237, 2024.
APA
Cui, P., Zhang, Y., Wang, C., Xiao, B., Wang, Q., Zhang, L. ... Tian, W. (2024). Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis. Experimental and Therapeutic Medicine, 27, 237. https://doi.org/10.3892/etm.2024.12525
MLA
Cui, P., Zhang, Y., Wang, C., Xiao, B., Wang, Q., Zhang, L., Li, H., Wu, C., Tian, W."Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis". Experimental and Therapeutic Medicine 27.5 (2024): 237.
Chicago
Cui, P., Zhang, Y., Wang, C., Xiao, B., Wang, Q., Zhang, L., Li, H., Wu, C., Tian, W."Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis". Experimental and Therapeutic Medicine 27, no. 5 (2024): 237. https://doi.org/10.3892/etm.2024.12525
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