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CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells

  • Authors:
    • He Bao
    • Chen Wang
    • Xiaorong Xue
    • Bin Hu
    • Qi Guo
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Pharmacy, The Second Affiliated Hospital of Air Force Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Pharmacy, Xi'an People's Hospital (Xi'an Fourth Hospital), Xi'an, Shaanxi 710004, P.R. China
    Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 268
    |
    Published online on: April 26, 2024
       https://doi.org/10.3892/etm.2024.12556
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Abstract

The anesthetic drug, ketamine (KTM) has been shown to induce therapeutic effects against major depressive disorder (MDD), however the related underlying mechanisms remain unclear. In the present study, HT22 neuronal cells were treated with glutamate to imitate oxidative stress injury in MDD, and it was hypothesized that the cannabinoid type 1 (CB1) receptor mediates KTM‑induced neuroprotection via ameliorating mitochondrial function in glutamate‑treated neuronal cells. Compared with the control, glutamate decreased cell viability and intracellular antioxidants, including glutathione (GSH), catalase and superoxide dismutase 2 levels, and inhibited mitochondrial function simultaneously. Moreover, glutamate increased lactate dehydrogenase release, cellular apoptosis level, cleaved caspase‑3 expression and intracellular oxidants, such as reactive oxygen species, oxidized GSH and mitochondrial superoxide in the cells. The presence of KTM, however, significantly decreased the glutamate‑induced oxidative stress injury, ameliorated the antioxidant/oxidant levels in the cells, enhanced mitochondrial function and upregulated CB1 receptor expression (P<0.05). Co‑administration of the CB1 receptor antagonist AM251 markedly abolished the KTM‑induced cytoprotective effects and ameliorations of antioxidant/oxidant levels and mitochondrial function, and also reversed CB1 upregulation (P<0.05). These observations indicated that KTM decreases the oxidative stress injury caused by glutamate in HT22 neuronal cells, and the neuroprotective effects may be mediated by the CB1 receptor.
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Copy and paste a formatted citation
Spandidos Publications style
Bao H, Wang C, Xue X, Hu B and Guo Q: CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells. Exp Ther Med 27: 268, 2024.
APA
Bao, H., Wang, C., Xue, X., Hu, B., & Guo, Q. (2024). CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells. Experimental and Therapeutic Medicine, 27, 268. https://doi.org/10.3892/etm.2024.12556
MLA
Bao, H., Wang, C., Xue, X., Hu, B., Guo, Q."CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells". Experimental and Therapeutic Medicine 27.6 (2024): 268.
Chicago
Bao, H., Wang, C., Xue, X., Hu, B., Guo, Q."CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells". Experimental and Therapeutic Medicine 27, no. 6 (2024): 268. https://doi.org/10.3892/etm.2024.12556
Copy and paste a formatted citation
x
Spandidos Publications style
Bao H, Wang C, Xue X, Hu B and Guo Q: CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells. Exp Ther Med 27: 268, 2024.
APA
Bao, H., Wang, C., Xue, X., Hu, B., & Guo, Q. (2024). CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells. Experimental and Therapeutic Medicine, 27, 268. https://doi.org/10.3892/etm.2024.12556
MLA
Bao, H., Wang, C., Xue, X., Hu, B., Guo, Q."CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells". Experimental and Therapeutic Medicine 27.6 (2024): 268.
Chicago
Bao, H., Wang, C., Xue, X., Hu, B., Guo, Q."CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells". Experimental and Therapeutic Medicine 27, no. 6 (2024): 268. https://doi.org/10.3892/etm.2024.12556
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