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Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade

  • Authors:
    • Guanqing Li
    • Suhuan He
    • Tao Liu
    • Na Zheng
  • View Affiliations / Copyright

    Affiliations: Emergency Department, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China, The First Outpatient Department of The Directly Affiliated Organ of Hebei Province, Shijiazhuang, Hebei 050000, P.R. China, Department of Plastic Surgery, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 277
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    Published online on: May 9, 2024
       https://doi.org/10.3892/etm.2024.12565
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Abstract

In the development of diabetic nephropathy, pathological damage such as interstitial fibrosis and cell apoptosis often occur in renal tubules. In the present study, diabetic and control group mice were randomly treated with vitamin D3 or vehicle for 6 months. In addition, human renal tubular epithelial (HK‑2) cells were cultured in high‑glucose medium and treated with vitamin D3 or the oxidative inhibitor NAC. Immunohistochemistry, western blotting, quantitative PCR), and ELISA showed that vitamin D3 decreased the expression of α‑smooth muscle actin and E‑cadherin in renal tubular epithelial cells, improving interstitial fibrosis. It also downregulated the ratio of Bax/Bcl2 protein, alleviating apoptosis in renal tubular epithelial cells. Furthermore, vitamin D3 significantly inhibited oxidative stress response in renal tubular epithelial cells and blocked the (Thioredoxin Interacting Protein) TXNIP/NLRP3 inflammatory pathway. Therefore, vitamin D3 can protect renal tubular epithelial cells from fibrosis and apoptosis by inhibiting oxidative stress response and blocking the TXNIP/NLRP3 inflammatory pathway in diabetic nephropathy.
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Copy and paste a formatted citation
Spandidos Publications style
Li G, He S, Liu T and Zheng N: Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade. Exp Ther Med 28: 277, 2024.
APA
Li, G., He, S., Liu, T., & Zheng, N. (2024). Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade. Experimental and Therapeutic Medicine, 28, 277. https://doi.org/10.3892/etm.2024.12565
MLA
Li, G., He, S., Liu, T., Zheng, N."Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade". Experimental and Therapeutic Medicine 28.1 (2024): 277.
Chicago
Li, G., He, S., Liu, T., Zheng, N."Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade". Experimental and Therapeutic Medicine 28, no. 1 (2024): 277. https://doi.org/10.3892/etm.2024.12565
Copy and paste a formatted citation
x
Spandidos Publications style
Li G, He S, Liu T and Zheng N: Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade. Exp Ther Med 28: 277, 2024.
APA
Li, G., He, S., Liu, T., & Zheng, N. (2024). Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade. Experimental and Therapeutic Medicine, 28, 277. https://doi.org/10.3892/etm.2024.12565
MLA
Li, G., He, S., Liu, T., Zheng, N."Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade". Experimental and Therapeutic Medicine 28.1 (2024): 277.
Chicago
Li, G., He, S., Liu, T., Zheng, N."Therapeutic potential of vitamin D3 in mitigating high glucose‑induced renal damage: Mechanistic insights into oxidative stress inhibition and TXNIP/NLRP3 signaling pathway blockade". Experimental and Therapeutic Medicine 28, no. 1 (2024): 277. https://doi.org/10.3892/etm.2024.12565
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