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Article

Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: In silico and in vitro experiments

  • Authors:
    • Aleksandra Nowakowska
    • Minjee Kim
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Science and Engineering, Konkuk University Convergence Science and Technology Institute, Konkuk University, Seoul 05029, Republic of Korea
  • Article Number: 106
    |
    Published online on: March 27, 2025
       https://doi.org/10.3892/etm.2025.12856
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Abstract

Influenza infections damage the airway and induce the innate immune response that contributes to hyper‑inflammation. Eucommiae Cortex (EC) enhances immune function and suppresses inflammation. To determine potential compounds and targets of EC associated with influenza, bioinformatics analyses and experimental verification were employed. The active compounds of EC were retrieved from the Traditional Chinese Medicine Systems Pharmacology database. The intersecting targets of EC and influenza were determined and examined using network pharmacology to analyze the relationship between the compounds and disease targets. The network identified three main compounds (quercetin, genistein and kaempferol) and four main targets (IL6, BCL2, IL1B and TNF). The ligand‑target binding affinity was calculated by molecular docking, a computational method used in drug design to predict the interaction between the compound and protein target. The docking results revealed that kaempferol and TNF showed the strongest binding affinity. In vitro experiments confirmed the therapeutic effect of EC in influenza virus‑infected Madin‑Darby canine kidney cells. Collectively, the present study identified the active compounds and potential targets of EC in influenza and suggested EC as a future influenza treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Nowakowska A and Kim M: Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments. Exp Ther Med 29: 106, 2025.
APA
Nowakowska, A., & Kim, M. (2025). Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments. Experimental and Therapeutic Medicine, 29, 106. https://doi.org/10.3892/etm.2025.12856
MLA
Nowakowska, A., Kim, M."Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments". Experimental and Therapeutic Medicine 29.5 (2025): 106.
Chicago
Nowakowska, A., Kim, M."Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments". Experimental and Therapeutic Medicine 29, no. 5 (2025): 106. https://doi.org/10.3892/etm.2025.12856
Copy and paste a formatted citation
x
Spandidos Publications style
Nowakowska A and Kim M: Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments. Exp Ther Med 29: 106, 2025.
APA
Nowakowska, A., & Kim, M. (2025). Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments. Experimental and Therapeutic Medicine, 29, 106. https://doi.org/10.3892/etm.2025.12856
MLA
Nowakowska, A., Kim, M."Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments". Experimental and Therapeutic Medicine 29.5 (2025): 106.
Chicago
Nowakowska, A., Kim, M."Deciphering the anti‑influenza potential of Eucommiae Cortex based on bioinformatics analysis: <em>In silico</em> and <em>in vitro</em> experiments". Experimental and Therapeutic Medicine 29, no. 5 (2025): 106. https://doi.org/10.3892/etm.2025.12856
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