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Article

Anti‑inflammatory effects of oridonin on an in vitro model using LPS‑treated human intestinal Caco‑2 cells

  • Authors:
    • Maonan Wang
    • Bo Xu
    • Linyue Li
    • Yu Wang
    • Mingcheng Li
  • View Affiliations / Copyright

    Affiliations: Department of Rectum Surgery, Jilin Cancer Hospital, Changchun, Jilin 130000, P.R. China, Department of Rectum Surgery, Jilin Provincial People's Hospital, Changchun, Jilin 130000, P.R. China, Department of Clinical Diagnosis, School of Medical Technology, Beihua University, Jilin, Jilin 132013, P.R. China, Department of Rectum Surgery, Jilin Cancer Hospital, Changchun, Jilin 130000, P.R. China, Department of Clinical Diagnosis, School of Medical Technology, Beihua University, Jilin, Jilin 132013, P.R. China
  • Article Number: 127
    |
    Published online on: March 3, 2026
       https://doi.org/10.3892/etm.2026.13121
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Abstract

Oridonin (Ori) attenuates dextran sulfate sodium‑induced ulcerative colitis (UC) in mice, and its underlying mechanism involves the sirtuin 1 (SIRT1)/NF‑κB/p53 pathway. In the present study, lipopolysaccharide (LPS)‑stimulated Caco‑2 cells were used as an in vitro model to mimic human intestinal epithelial inflammation, to ascertain the anti‑inflammatory effects of Ori on a cell model of UC. The concentrations of TNF‑α and IL‑1β were quantified using ELISA, the expression levels of SIRT1, NF‑κB and p53 were assessed through western blot analysis, and cell viability was determined using the Cell Counting Kit‑8. The results showed that cell viability was affected by treatment with Ori at different doses. Ori significantly inhibited the optical density values in a dose‑dependent manner at the doses of 5.0‑80.0 µM whereas it did not affect viability at the doses of 0.625‑2.50 µM, compared with in the control group. These findings suggested that the inhibitory effect of Ori on intestinal epithelial cells did not result from cytotoxic action at the doses of 0.625‑2.50 µM. Furthermore, Ori suppressed the inflammatory response in intestinal epithelial cells by significantly reducing LPS‑induced secretion of the pro‑inflammatory cytokines TNF‑α and IL‑1β with a ~2‑fold reduction. Additionally, Ori significantly increased the protein expression levels of SIRT1, and decreased the protein expression levels of NF‑κB and p53. In conclusion, the present results indicated that the inhibition of NF‑κB‑mediated inflammation may be a key mechanism by which Ori exerts its therapeutic effects on UC.
View Figures

Figure 1

Effect of oridonin on the viability of
Caco-2 cells. **P<0.01. NC, normal control; ns, not
significant.

Figure 2

Ori inhibits the secretion of
pro-inflammatory mediators TNF-α and IL-1β in LPSexposed,
differentiated Caco-2 cells. (A) Densitometric analysis of TNF-α
levels. (B) Densitometric analysis of IL-1β levels.
**P<0.01. H, high-dose; L, low-dose; LPS,
lipopolysaccharide; NC, normal control; Ori, oridonin.

Figure 3

Effect of Ori on the protein
expression levels of SIRT1, NF-κB and p53 in LPSexposed,
differentiated Caco2 cells. (A) SIRT1, NF-κB and p53 were detected
by western blot analysis, with GAPDH as the internal control.
Densitometric analysis of (B) p53/GAPDH, (C) SIRT1/GAPDH, and (D)
NF-κB/GAPDH. **P<0.01. H, high-dose; L, low-dose;
LPS, lipopolysaccharide; NC, normal control; Ori, oridonin.
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Copy and paste a formatted citation
Spandidos Publications style
Wang M, Xu B, Li L, Wang Y and Li M: Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells. Exp Ther Med 31: 127, 2026.
APA
Wang, M., Xu, B., Li, L., Wang, Y., & Li, M. (2026). Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells. Experimental and Therapeutic Medicine, 31, 127. https://doi.org/10.3892/etm.2026.13121
MLA
Wang, M., Xu, B., Li, L., Wang, Y., Li, M."Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells". Experimental and Therapeutic Medicine 31.5 (2026): 127.
Chicago
Wang, M., Xu, B., Li, L., Wang, Y., Li, M."Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells". Experimental and Therapeutic Medicine 31, no. 5 (2026): 127. https://doi.org/10.3892/etm.2026.13121
Copy and paste a formatted citation
x
Spandidos Publications style
Wang M, Xu B, Li L, Wang Y and Li M: Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells. Exp Ther Med 31: 127, 2026.
APA
Wang, M., Xu, B., Li, L., Wang, Y., & Li, M. (2026). Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells. Experimental and Therapeutic Medicine, 31, 127. https://doi.org/10.3892/etm.2026.13121
MLA
Wang, M., Xu, B., Li, L., Wang, Y., Li, M."Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells". Experimental and Therapeutic Medicine 31.5 (2026): 127.
Chicago
Wang, M., Xu, B., Li, L., Wang, Y., Li, M."Anti‑inflammatory effects of oridonin on an <em>in vitro</em> model using LPS‑treated human intestinal Caco‑2 cells". Experimental and Therapeutic Medicine 31, no. 5 (2026): 127. https://doi.org/10.3892/etm.2026.13121
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